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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2006; 52(6): 52-56


Effect of highdispersed silicaon the cultured rat hepatocytes and their death pathways

N. Rozmaritsa, N. Makogon, I. Gerashchenko, I. Alexeyeva

    Bogomoletz Institute of Physiology, Kyiv;Institute of surface chemistry, Kyiv


Abstract

Highdispersed silica (HDS) is an active substance of medicine “Siliks”, which demonstrates high protein adsorption prop- erty and is used for hatching out toxic agents of protein origin. To discover new potential applications of this substance, it is necessary to study the direct effect of HDS on cell viability. We studied the effects of HDS in concentration 0,0001%, 0,001% and 0,01% on cultured rat hepatocytes at 4 and 24 hours. To estimate the number of alive vs. apoptotic and ne- crotic cells, fluorescent nucleic dyes Hoechst 33342 and propidium iodide were employed. Cells that underwent au- tophagic processes were estimated using a specific fluorescent autophagosome marker monodansylcadaverine. We show here that HDS has dose-dependent effect on cell death; the number of cells with apoptotic features increased after 4 hours and in greater extend after 24 hours of treatment with HDS. At the lowest concentration HSD did not significantly affect cell vi- ability, and we observed decrease in postapoptotic necrosis in cell culture. The highest concentration of HSD dramatically increased cell death through both necrosis and apoptosis. At the same time, autophagic activity was suppressed by 0,01% HSD.

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