Effect of highdispersed silicaon the cultured rat hepatocytes and their death pathways
N. Rozmaritsa, N. Makogon, I. Gerashchenko, I. Alexeyeva
Bogomoletz Institute of Physiology, Kyiv;Institute of surface chemistry, Kyiv
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Abstract
Highdispersed silica (HDS) is an active substance of medicine
“Siliks”, which demonstrates high protein adsorption prop-
erty and is used for hatching out toxic agents of protein origin.
To discover new potential applications of this substance, it is
necessary to study the direct effect of HDS on cell viability.
We studied the effects of HDS in concentration 0,0001%,
0,001% and 0,01% on cultured rat hepatocytes at 4 and 24
hours. To estimate the number of alive vs. apoptotic and ne-
crotic cells, fluorescent nucleic dyes Hoechst 33342 and
propidium iodide were employed. Cells that underwent au-
tophagic processes were estimated using a specific fluorescent
autophagosome marker monodansylcadaverine. We show here
that HDS has dose-dependent effect on cell death; the number
of cells with apoptotic features increased after 4 hours and in
greater extend after 24 hours of treatment with HDS. At the
lowest concentration HSD did not significantly affect cell vi-
ability, and we observed decrease in postapoptotic necrosis in
cell culture. The highest concentration of HSD dramatically
increased cell death through both necrosis and apoptosis. At
the same time, autophagic activity was suppressed by 0,01%
HSD.
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