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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

(English title: Physiological Journal)

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2026; 72(4): 53-58

SIRTUIN 1 ACTIVATION IS A PROMISING STRATEGY FOR REDUCING DNA DAMAGE IN MOUSE OOCYTES FOLLICULAR CELLS

V.O. Sribna1, O.T. Blashkiv1, Yu.V. Perepelitsa2, T.Yu. Voznesenska1

  1. Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
  2. Bogomolets National Medical University, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz72.04.053


Abstract

Research aimed at understanding DNA repair mechanisms in both damaged and normal cells remains highly relevant. The aim of this study was to investigate how activation of sirtuin 1 affects the distribution of DNA single-strand breaks in mouse oocyte follicular cells. The alkaline comet assay was used, in which, during electrophoresis of lysed cells embedded in agarose gel, loops and fragments of damaged DNA migrate toward the anode, producing a characteristic comet-like ap- pearance. The length of the comet tail positively correlates with the extent of DNA damage (nuclei were classified into damage classes 0-4). Under conditions of oxidative stress, the proportions of class 3 and class 4 nuclei increased by 2.6-fold and 7.8-fold, respectively, whereas the proportions of class 0/1 and class 2 nuclei decreased by 18.6-fold and 2.3-fold, respec- tively, compared with control cells. These findings indicate a redistribution of DNA single-strand breaks in follicular cells under oxidative stress. Resveratrol, a sirtuin 1 activator, was found to promote DNA repair, as evidenced by an increase in the proportion of cells with class 0/1 nuclei and a decrease in the proportion of cells with class 3 and class 4 nuclei. The obtained results provide evidence that sirtuin 1 activation represents a promising strategy for reducing DNA damage in mouse oocyte follicular cells.

Keywords: oocyte follicular cells; DNA single-strand breaks; oxidative stress; sirtuin 1

Full text: (PDF, in Ukrainian)

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