Generalized purulent peritonitis (GPP) remains one of the most pressing problems in modern abdominal surgery. Our study aimed to investigate the dynamics of biochemical blood parameters in rats during treatment with Ag/Cu nanocomposites and low-frequency ultrasound for experimental GPP. The study was conducted on Wistar rats with simulated GPP, which were divided into three experimental groups of 20 rats each. GPP was simulated using a mixture of multidrug-resistant bacteria: S. aureus, E. coli, and P . aeruginosa. In the 1st group, abdominal cavity sanitation was performed using a solution of Ag/Cu nanocomposites; in the 2nd group, it was carried out using low-frequency ultrasound and physiological saline; and in the 3rd group, Ag/Cu nanocomposites combined with low-frequency ultrasound were used. Starting from the 7th day of the experiment, in the group with the combined use of Ag/Cu nanocomposites and low-frequency ultrasound, compared to the 1st group, the biochemical blood parameters of rats significantly improved: total protein, creatinine, alanine aminotransferase, fibrinogen, C-reactive protein, α 1 -globulins, α 2 -globulins, and albumin-globulin ratio. On the 14th day, further normalization of biochemical parameters was observed in the 3rd group receiving the combined therapy. Among these, the levels of total protein, creatinine, total bilirubin, alanine aminotransferase, C-reactive protein, α 2 -globulins, and the albumin-globulin ratio differed significantly from those of the 1st and 2nd groups. Thus, the combined use of Ag/Cu nanocomposites and low-frequency ultrasound accelerates the normalization of biochemical blood parameters by effectively sanitizing the abdominal cavity. This approach reduces the systemic inflammatory response, eliminates endogenous intoxication, and restores the functional state of parenchymal organs, which is pathogenetically justified for the treatment of GPP.