THE INFLUENCE OF MULTIPROBIOTIC ON THE METABOLISM OF SEROTONIN IN THE BLOOD SERUM AND DUODENUM OF RATS WITH GLUTAMATE-INDUCED OBESITY
M.M. Kondro1, T.I. Galenova2, O.M. Savchuk2
- State Non-Commercial Enterprise “Danylo Halytsky Lviv National Medical University”, Ukraine
- Educational and Scientific Center “Institute of Biology and Medicine” of Taras Shevchenko National University of Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz72.02.049
Abstract
Data on serotonin content and its metabolism in serum and
duodenum in rats with glutamate-induced obesity (GIO)
are limited. The work aimed to investigate the prophylactic
effect of periodic course administration of the multiprobiotic
“Symbiter acidophilus” concentrated on the content of
serotonin and indicators of its metabolism in the blood
serum and duodenum in rats with GIO. It was found that the
concentration of serotonin and tryptophan in the blood serum
of rats with GIO decreased against the background of an
increase in monoamine oxidase activity. Under the influence of
periodic administration of a multiprobiotic to rats after neonatal
administration of monosodium glutamate, the concentration
of serotonin in the blood serum increased, although it did not
reach the control level. And the concentration of tryptophan
in the blood serum in this group of rats was restored to the
level of the intact control, which contributed to an increase
in the concentration of serotonin. One of the reasons for the
increase in the concentration of serotonin in the blood serum
in rats against the background of periodic administration of a
multiprobiotic after neonatal administration of monosodium
glutamate was a decrease in the activity of monoamine
oxidase. In rats with GIO, the content of serotonin in the
duodenal homogenate increased with a simultaneous decrease
in the content of tryptophan and an increase in the activity of
monoamine oxidase in comparison with the control group
of rats. In rats, after neonatal administration of monosodium
glutamate against the background of periodic administration
of a multiprobiotic in the duodenal homogenate, the serotonin
content decreased, the tryptophan content increased, and the
monoamine oxidase activity decreased compared with the
group of rats after neonatal administration of monosodium
glutamate. In the duodenal homogenate of rats with GIO,
tryptophan hydroxylase and indoleamine-2,3-dioxygenase
activities decreased, and tryptophan decarboxylase activity
did not undergo statistically significant changes. In rats, after
neonatal administration of monosodium glutamate against
the background of periodic administration of a multiprobiotic
in the duodenal homogenate, tryptophan hydroxylase and
indoleamine-2,3-dioxygenase activities increased, and
tryptophan decarboxylase activity did not change.
Keywords:
obesity, monosodium glutamate, serotonin, blood serum, duodenum, multiprobiotic
References
1. Urbanovich AM, Lanyush FV. The role of grelin and serotonin in the control of food behaviour in patients with obesity and diabetes melitus II type. Mìžnarod Endokrinol Žurn. 2020;16(2):145-51. doi: 10.22141/2224- 0721.16.2.2020.201300.
2. Haleem DJ, Mahmood K. Brain serotonin in high-fat diet-induced weight gain, anxiety and spatial memory in rats. Nutrit Neurosci. 2019 May;24(3): 226-35. doi: org/10.1080/ 1028415X.2019.1619983.
3. Van Galen KA, Ter Horst KW, Serlie MJ. Serotonin, food intake, and obesity. Obes Rev. 2021 Jul;22(7):e13210. doi: 10.1111/obr.13210.
4. Moon JH, Oh C-M, Kim H. Serotonin in the regulation of systemic energy metabolism. J Diabet Investig. 2022; 13:1639-45. doi: 10.1111/jdi.13879.
5. Bakovic P, Ktsic M, Kolaric D, Stefulj J, Cicin-Sain L. Metabolic and molecular response to high-fat diet differs between rats with constitutionally high and low serotonin tone. Int J Mol Sci. 2023 Jan; 24(3):2169. doi: 10.3390/ ijms24032169.
6. Kayode OT, Bello JA, Oguntola JA, Kayode AAA, Olukoya DK. The interplay between monosodium glutamate (MSG) consumption and metabolic disorders. Heliyon. 2023SeP; 9(9):e19675. doi:10.1016/j.heliyon.2023. e19675.
7. Bera TK, Kar SK, Yadav PK, Mukherjee P, Yadav S, Joshi B. Effects of monosodium glutamate on human health: A systematic review. World J Pharm Sci. 2017:139-44.
8. Armstrong WR, Gafita A, Zhu S, Thin P, Nguyen K, Alano RM, et al. The impact of monosodium glutamate on 68Ga-PSMA-11 biodistribution in men with prostate cancer: a prospective randomized, controlled, imaging study. J Nucl Med. 2021;62(4):1244-51. doi: 10.2967/ jnumed.120.257931.
9. Martina AM, Yabutb JM, Chooa JM, PageeAJ, Suna EW, JessuPa CF, et al. The gut microbiome regulates host glucose homeostasis via peripheral serotonin. PNAS. 2019 SeP;16(40):19802-4. www.Pnas.org/cgi/doi/10.1073/ Pnas.1909311116,
10. Geng J, Ni Q, Sun W, Li L, Feng X. The links between gut microbiota and obesity and obesity related diseases. Biomed Pharmacother. 2022 Mar:147:112678. doi:
10.1016/j.bioPha.2022.112678.
11. Takai A, Kikuchi K, Ichimura M, Tsuneyama K, Moritoki Y, Matsumoto K, et al. Fructo-oligosaccharides ameliorate steatohepatitis, visceral adiposity, and associated chronic inflammation via increased production of shortchain fatty acids in a mouse model of non-alcoholic steatohepatitis. BMC Gastroenterol. 2020 Feb; 20:46. doi: 10.1186/s12876-020-01194-2.
12. Sanabria ER, Pereira MF, Dolnikoff MS, Andrade IS, Ferreira AT, Cavalheiro EA, Fernandes MJ. Deficit in hippocampal long-term potentiation in monosodium glutamate-treated rats. Brain Res Bull. 2002; 59: 47-51.
13. Nakanishi Y, Tsuneyama K, Fujimoto M, Salunga TL, Nomoto K, An J-L, et al. Monosodium glutamate (MSG): a villain and promoter of liver inflammation and dysplasia. J Autoimmun. 2008; 30(1-2): 42-50.
14. Kobyliak N, Falalyeyeva T, Virchenko O, Mykchalchyshyn G, Bodnar P, Spivak M, Yankovski D, Beregova T, OstaPchenko L. Comparative experimental investigation on the efficacy of mono- and multiprobiotic strains in non-alcoholic fatty liver disease prevention. BMC Gastroenterol. 2016; 16:34.
15. Bernardis LL, Patterson BD. Correlation between “Li index” and carcass fat content in weanling and adult female rats with hypothalamic lessions. J Endocrinol. 1968; 40: 527-8.
16. Gаіtоndе MK. А fluоrіmеtrіc mеthоd fоr thе dеtеrmіnаtіоn оf trypоphаn іn аnіmаl tіssuеs. Bіоchеm J. 1974; 139: 625-31.
17. Maksimenko EG, Savchenko VN. The level of tryptophan and serotonin in the convulsive readiness conditions of cerebrum. Visn Kharkov Natl V.N. Karazin Univ. Ser Medicine. 2000; 1(494): 40-3.
18. Wеіssbаch H, Wааlkеs TP, Udеnfrіеnd S. А sіmptіfіеd mеthоd fоr mеаsurіng sеrоtоnіn іn tіssuе; sіmultаnеоus аssаy оf bоth sеrоtоnіn аnd hіstаmіnе. J Bіоl Chеm. 1957; 230(2):865-71.
19. Ostapchenko LI, Mykhailyk IV. Biological membranes: methods for studying structure and functions: Textbook. Kyiv: Publishing and Printing Center «Kyiv University». 2006.
20. Kuhn DM, О’Cаllаghаn JP, Juskеvіch J, Lovenberg W. Аctіvаtіоn оf brаіn tryptоphаn hydrоxylаsе by АTP-Mg2+: Dеpеndеncе оn cаlmоdulіn. Bіоchеmіctry. 1980;77:4688-91.
21. Ahlersova E, Pastorova B, Rassayova M, Ahlers I, Smajda B. Reduced pineal melatonin biosynthesis in fractionally irradiated rats. Physiol Res. 1998;47:133-6.
22. Sаngwаn R, Mіshrа S, Kumаr S. Dіrеct fluоrоmеtry оf phаsе-еxtrаctеd tryptаmіnе-bаsеd fаst quаntіtаtіvе аssаy оf L-tryptоphаn dеcаrbоxylаsе frоm cаthаrаnthus rоsеus lеаf. Аnаlyt Bіоchеm. 1998;255(1):39-46.
23. Kudо Y, Boyd CAR, Sargent IL. Mоdulаtіоn оf іndоlеаmіnе-2,3-dіоxygеnаsе by іntеrfеrоn-γ іn humаn plаcеntаl chоrіоnіc vіllі. Mоl Humаn Rеprоduct. 2000;6(4):369-74.
24. Kondro MM, Galenova TI, Savchuk OM, Beregova TV. Serotonin metabolism in the brain of rats with glutamateinduced obesity on the background of periodic adminis tration of a multiprobiotic. Fiziol Zh. 2025;71(6):59-66. doi: CrossRef [Ukrainian].
25. Crane JD, Palanivel R, Mottillo EP, Bujak AL, Wang H, Ford RJ, et al. Inhibiting peripheral serotonin synthesis reduces obesity and metabolic dysfunction by promoting brown adipose tissue thermogenesis. Nat Med. 2015;21(2):166-72.
26. Saeed FMY, Jasim RF. Indoleamine-2,3-Dioxygenase level and oxidative stress parameters in the serum of patients with chronic renal failure. Ukr Biochem J. 2023;95(4):17-
23. doi: CrossRef
27. Tom-Castro XM, Rodriguez-Arrastia M, Cardona D, Rueda-Ruzafa L, Molina-Torres G, Roman P. Probiotics as a therapeutic strategy in obesity and overweight: a systematic review. Benef Microb. 2021 Feb;12(1):5-15. doi: 10.3920/BM2020.0111.
|
