Lipopolysaccharide triggers an inflammatory response, oxidative stress, alterations in protein and receptor expression, and disruptions to metabolic pathways, all of which affect physiological processes in the uterus and impair the reproductive ability of animals. Endotoxin significantly increases oxytocin-induced uterine contractile activity, which can disrupt the course of pregnancy and cause premature birth. Our work aimed to determine the mRNA expression of genes encoding oxytocin receptors, antioxidant and H 2 S-synthesizing enzymes, as well as the Kir6.x subunit of ATP-sensitive potassium channels (K ATP channels) in uterine tissues of female rats after intraperitoneal administration of lipopolysaccharide and glutathione. mRNA expression was determined by reverse transcription and real-time PCR. Lipopolysaccharide was administered intraperitoneally at a dose of 3 mg/kg one day before removing the animals from the experiment. Glutathione was injected intraperitoneally at a dose of 52 mg/kg twice: one hour before lipopolysaccharide administration and one day later. It was shown that lipopolysaccharide led to a significant increase in the expression levels of mRNA of genes encoding oxytocin receptors (by 4.6 times), the antioxidant enzyme catalase (by 3.3 times), the H 2 S-synthesizing enzyme cystathionine-γ-lyase (by 5.8 times), and the Kir6.1 subunit of K ATP channels (by 2.4 times) in rat uterine tissue, and at the same time reduced the expression levels of the antioxidant enzyme superoxide dismutase (by 38%). Glutathione normalized the expression of oxytocin receptors, superoxide dismutase, and catalase to control values, partially prevented the increase in the expression of cystathionine-γ-lyase, had practically no effect on the expression of Kir6.1, and, conversely, suppressed the expression of Kir6.2 (by 3.6 times). Thus, the early stages of Вплив глутатіону на регуляторні та захисні сигнальні шляхи при ендотоксемії у матці щурів 59 lipopolysaccharide-induced endotoxemia are characterized, on the one hand, by the development of inflammation, oxidative stress, and increased expression of oxytocin receptors, and on the other hand, by the activation of protective processes in the body as a mechanism of adaptation to the inflammation. Glutathione prevented lipopolysaccharide-induced changes, suggesting its possible use in endotoxemia to prevent reproductive disorders in animals.