Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2009; 55(1): 36-42


T.V. Martynova, I.N. Alexeyeva

    O.O. Bogomoletz Institute of Physiology National Academy Sciences of Ukraine, Kyiv, Ukraine


The aim of present work was to compare the functional activity of peritoneal macrophages (Mf) at T-cellular and antibody in­duced hepatitis in mice of CBA line. T-cellular hepatitis was caused by concanavalin A (ConA), antibody-induced hepatitis was caused by administration of xenogenic anti-liver antibo­dies: gamma-globulin fractions of antihepatocytotoxic serum (g-AHCS). It was found that single injection of ConA or g-AHCS caused damage of liver with cytolytic syndrome through 20 hours. Functional activity of Mf in these conditions was sig­nificantly different. Application of ConA resulted in the decrease in phagocytosis of latex particles and oxygen-dependent me­tabolism; application of g-AHCS - to increase of these processes. Weakening of Mf activity may be one of the reasons for the decrease of dead cell eliminations that results in the maintenance of inflammatory reaction. At the same time significant amplification of phagocytic Mf activity may be one of the path­ways of free radical endogenic sources increase that causes cell alteration and plays its role as mediators at inflammation.


  1. Alekseeva IN, Bryzgana TM, Pavlovich SI, Ilchevich NV Liver and immunological reactivity K .: Nauk. opinion, 1991. 168 p.
  2. Alekseeva IM, Brizgina TM, Aleksyuk LI etc. The role of nitric oxide in the development of humoral immune response in mice. Fiziol Zh. 2005. 51, N 4. p. 13-19.
  3. Bryukhin GV Grachev F.Yu. Intensity of Fc-dependent phagocytosis of peritoneal macrophages and peripheral blood monocytes in the offspring of female rats with chronic liver disease . Ibid. 1991. 37, N 6. P.91-95.
  4. Drozhennikov VA, Lyashenko VA, Surgai VV Dynamics of activity of lysosomal hydrolases of mouse lymphocytes under the action of concanavalin A . Immunology. 1984. N 4. P.19-22.
  5. Zenkov NK, Menshikova EB, Shkurupiy VA Mechanisms of macrophage activation . Uspekhi sovrem. biology. 2007. 127, N 3. P.243-256.
  6. Zinovieva VN, Ostrovsky OV Free radical oxidation of DNA and its biomarker oxidized guanosine (8-oxod G) . Questions of honey. chemistry. 2002. 48, N 5. p. 419-431.
  7. Ivashkin VT, Bueverov AO Autoimmune liver diseases in the practice of a clinician. M .: M. Vesti, 2001. 98p.
  8. Immunological methods . Ed. G. Frimel. M .: Medicine, 1987, 472 p.
  9. Kulinsky VI Biochemical aspects of inflammation . Biochemistry. 2007. 72, N 6. P.733-746. CrossRef PubMed
  10. Makogon NV, Pavlovich SI, Bryzgina TM etc. Proliferation and death of mononuclear cells of the liver of mice under the conditions of its immune damage caused by the introduction of concanavalin A or antihepatic antibodies. Fiziol Zh. 2008. 54, N 6. P.49-57.
  11. Martinova TV Changes in the functional activity of T-lymphocytes and their subpopulations (helpers and suppressors) in the immune response to sheep erythrocytes under conditions of liver damage by carbon tetrachloride. Author's ref. dis ... cand. biological sciences Kyiv, 1996. 23 p.
  12. Martinova TV, Aleksyuk LI Functional activity of peritoneal macrophages in the liver of mice with concanavalin A . Physiol. magazine. 2007. 53, N 5. p. 47-52.
  13. Mayansky AN, Viksman ME, Kotelnikov PN, Molchanova IV Characteristics of the functional activity of human blood neutrophils by the reaction of reduction of nitrosine tetrazolium . Journal. microbiology. 1977. N 6. p. 108-111.
  14. Novikov DK, Novikova VI Cellular methods of immunodiagnostics. Minsk: Belarus, 1979. 223 p.
  15. Pavlovich SI. Histostructural changes and regenerative processes in the liver under conditions of experimental autoimmune hepatitis . Achievements clip. and experiment. medicine. 2007. N 2. P.118-120.
  16. Podoprigorova VG, Tsygankova GM, Farashchuk NF Peculiarities of cytolytic syndrome in patients with chronic diffuse liver disease depending on the severity of the pathological process . Klin. lab. diagnostics. 2006. N 1. P.15-17.
  17. Proskuryakov S.Ya., Gabay VL, Konoplyanikov AG etc. Immunology of apoptosis and necrosis . Biochemistry. 2005. 70, N 12. p. 1593-1605. CrossRef PubMed
  18. Shubich MG, Mednikova VG NST-test in children with normal and purulent-bacterial infections . Lab. case. 1978. N 9. p. 515-518.
  19. Dean R.T., Fu S., Stocker R., Davies M.J. Biochemis­try and pathology of radical-mediated protein oxidation. Biochem J. 1997. 324 ( Pt 1). P. 1-18. CrossRef PubMed PubMedCentral
  20. Jirillo E., Caccavo D., Magrone T. et al. The role of the liver in the response to LPS: experimental and clinical findings .J. Endotoxin Res. 2002, 8(5). P. 319-327. CrossRef PubMed
  21. Kontorinis N., Agarwal K., Elhajj N. et al. Pegylated interferon-induced immune-mediated hepatitis post-liver transplantation . Liver Transpl. 2006. 12(5). P. 827-830. CrossRef PubMed
  22. Lich R., Dieker J.W., Jacobs C.W. et al. Decreased phagocytosis of apoptosis cell in diseased SLE mice . J. Autoimmun. 2004. 22(2). P. 139-145. CrossRef PubMed
  23. Mivagi T., Takehara T., Tatsumi T. et al. Concanavalin a injection activates intrahepatic innate immune cells to provoke an antitumor effect in murine liver .Hepatology. 2004, 40(5). P. 1190-1196. CrossRef PubMed
  24. Mizuhara H., O'Neill E., Seki N. et al. Tcell activation-associated hepatic injury: mediation by tumor necrosis factors and protection by interleukin 6 .J. Exp.Med. 1994. 179(5). P. 1592-1537. CrossRef PubMed PubMedCentral
  25. Okamoto S., Yokohama S., Yoneda M. et al. Macroph­age inflammatory protein-1 alpha plays a crucial role in concanavalin A-induced liver injury through induction of proinflammatory cytokines in mice .Hepatol. Res. 2005. 32(1). P. 38-45. CrossRef PubMed
  26. Okamoto T., Nakano Y, Asakura W. et al. Expression of cytokine mRNA in extrahepatic organs in a mouse concanavaline A hepatitis model .Jpn. J. Pharmacol. 1998. 77(3). P. 219-225. CrossRef PubMed
  27. Prandota J. Possible pathomechanism of autoimmunehepatitis . Amer. J. Therap. 2003. 10(1). P.51-57. CrossRef PubMed
  28. Salmon J.E., Kimberly R.P., Gibofsky A., Fotino M. Altered phagocytosis by monocytes from HLA-DR2 and DR3-positive healthy adults is Fc gamma receptor spe­cific .J. Immunol. 1986. 136(10). P. 3625-3630.
  29. Sodhi A., Kesherwani V. Signaling molecules involved in production and regulation of IL01 beta by murine peri­toneal macrophages in vitro on treatment with con-canavalin A . Int. Immunopharmacol. 2007. 7(11). P. 1403-1413. CrossRef PubMed
  30. Tanaka H, Tujioka H, Ueda H. et al. Autoimmune hepa­titis triggered by acute hepatitis A . World J. Gastroenterol. 2005. 11(38). P. 6069-6071. CrossRef PubMed PubMedCentral
  31. Tieges G., Hentschel J., Wendel A. A T cell-dependent experimental liver injury in mice inducible by concanava-lin A .J. Clin. Invest. 1992. 90(1). P. 196-203. CrossRef PubMed PubMedCentral
  32. Tieges G. Experimental hepatitis and role of cytokines . . Acta Gastroenterol. Belg. 1997. 60(2). P. 176-179.
  33. Trautwein C., Rakermann T., Malek N.P. et al. Con- canavalin A-induced liver injury triggers hepatocyte prolif­eration .J. Clin. Invest. 1998. 101(9). P. 1960-1969. CrossRef PubMed PubMedCentral
  34. Vento S., Garofano T., Diperri G. et al. Identification of hepatitis A virus as a trigger for autoimmune chronic hepatitis type 1 in susceptible individuals . Lance . 1991. 337. P. 1183-1187. CrossRef
  35. Wolf D., Hallmann R., Sass G. et al. TNF-alpha-induced expression of adhesion molecules in the liver is under the control of TNFR1-relevance for concanavaline A-induced hepatitis .J.Immunol. 2001. 166(2). P.1300-1307. CrossRef PubMed
  36. Zachou K, Rigopoulou E, Dalekos G.N. Autoantibodies and autoantigens in autoimmune hepatitis:important tools in clinical practice and to study pathogenesis of the dis­ease .J. Autoimmune Dis. 2004. 1(1):2. P. 1-17. CrossRef PubMed PubMedCentral
  37. Zhan H.G., Mountz J.D., Fleck M. et al. Specific dele­tion of autoreactive T cells by adenovirus transfected, Fas ligand producing antigen presenting cells . Immunol.Res. 2002. 26(1-3). P. 235-246. CrossRef

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.