A hyperpolarization-activated current in rat menthol-sensitive sensory neurons
A. P. Kondratskyi, K. O. Kondratska, G. V. Sotkis, V.G. Naidenov, Ia. M. Shuba.
International Center of Molecular Physiology, NationalAcademy of Sciences of Ukraine, Kyiv;O.O. Bogomoletz Institute of Physiology, NationalAcademy of Sciences of Ukraine, Kyiv
Abstract
In the present study we have investigated the correlation be-
tween hyperpolarization-activated current (Ih) and menthol-ac-
tivated current (ITRPM8) in rat dorsal root ganglion (DRG) neurons.
We showed that Ih is present in 89% of menthol-sensitive neurons
which makes its presence reliable, though not absolute, criterion
for pre-selection of such neurons. Endogenous Ih recorded from
different neurons exhibited variable density and activation
kinetics. Based on the analysis of Ih activation kinetics we hy-
pothesize that the population of hyperpolarization-activated
channels in menthol-sensitive DRG neurons is mainly represented
by HCN1, HCN2 and HCN3 channels. The expression of
HCN4 isoform in these cells is very low.
References
- Accili E., Proenza C., Baruscotti M., DiFrancesco D. From funny current to HCN channels: 20 years of exci- tation // News Physiol. Sci. – 2002. – 17. – P.32–37.
- Babes A., Zorzon D., Reid G. A novel type of cold- sensitive neuron in rat dorsal root ganglia with rapid adaptation to cooling stimuli // Eur. J. Neurosci. – 2006. –24, № 3. – P. 691–698.
- Chaplan S., Guo H., Lee D et al. Neuronal hyperpolar- ization-activated pacemaker channels drive neuropathic pain // J. Neurosci. – 2003. – 23, № 4. –P.1169–1178.
- Chen S., Wang J., Siegelbaum S. Properties of Hyper- polarization-activated pacemaker current defined by coassembly of HCN1 and HCN2 subunits and basal modulation by cyclic nucleotide // J. Gen. Physiol. – 2001. – 117, № 5. – P.491–504.
- de la Pena E., Malkia A., Cabedo H., Belmonte C., Viana F. The contribution of TRPM8 channels to cold sensing in mammalian neurones // J. Physiol. – 2005. – 567, № 2. – P. 415–426.
- Dhaka A., Viswanath V., Patapoutian A. Trp ion channels and temperature sensation // Annu. Rev. Neurosci. – 2006. – 29. – P. 135–161.
- DiFrancesco D. Pacemaker mechanisms in cardiac tis- sue // Annu. Rev. Physiol. – 1993. – 55. – P. 455–472.
- Fonfria E., Murdock P.R., Cusdin F.S. et al. Tissue dis- tribution profiles of the human TRPM cation channel family // J. Rec. Signal. Transduct. Res. – 2006. – 26, № 3. – P. 159–178.
- 9. Kraft R., Harteneck C. The mammalian melastatin-related transient receptor potential cation channels: an overview / / Pflug. Arch. – 2005. – 451, № 1. – P. 204–211.
- 10. Ludwig A., Zong X., Jeglitsch M. et al. A family of hy- perpolarization-activated mammalian cation channels // Nature. – 1998. – 11. – 393, № 6685. – P. 587–591.
- Maingret F., Lauritzen I., Patel A.J. et al. TREK-1 is a heat-activated background K(+) channel // EMBO J. – 2000. – 1. – 19, № 11. – P. 2483–2491.
- McKemy D.D., Neuhausser W.M., Julius D. Identifi- cation of a cold receptor reveals a general role for TRP channels in thermosensation // Nature. – 2002. – 416, № 6876. – P. 52–58.
- Moosmang S., Stieber J., Zong X. et al. Cellular ex- pression and functional characterization of four hyper- polarization-activated pacemaker channels in cardiac and neuronal tissues // Eur. J. Biochem. – 2001. – 268, № 6. – P.1646–1652.
- Pape H.C. Queer current and pacemaker: the hyperpolarization-activated cation current in neurons // Annu. Rev. Physiol. – 1996. – 58. – P. 299–327.
- Pedersen S.F., Owsianik G., Nilius B. TRP channels: an overview // Cell Calcium. – 2005. – 38, № 3–4. – P. 233–252.
- Petruska J.C., Napaporn J., Johnson R.D. et al. Subclassified acutely dissociated cells of rat DRG: histochemistry and patterns of capsaicin-, proton-, and ATP-activated currents // J. Neurophysiol. – 2000. – 84, № 5. – P. 2365–2379.
- Pierau F.K., Torrey P., Carpenter D. Effect of ouabain and potassium-free solution on mammalian thermosensitive afferents in vitro // Pflug. Arch. – 1975. – 29. – 359, № 4. – P. 349–356.
- Raid G., Babes A., Pluteanu F. A cold- and mentholactivated current in rat dorsal root ganglion neurones: properties and role in cold transduction // J. Physiol. – 2002. – 545, № 2. – P.595–614.
- 19. Schдfer K., Braun H.A. Modulation of periodic cold receptor activity by ouabain // Pflugers Arch. – 1990. – 417, № 1. – P. 91–99.
- 20. Viana F., de la Peсa E., Belmonte C. Specificity of cold thermotransduction is determined by differential ionic channel expression // Nat. Neurosci. – 2002. – 5, № 3. – P. 254–260.
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