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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2008; 54(4): 16-22


A hyperpolarization-activated current in rat menthol-sensitive sensory neurons

A. P. Kondratskyi, K. O. Kondratska, G. V. Sotkis, V.G. Naidenov, Ia. M. Shuba.

    International Center of Molecular Physiology, NationalAcademy of Sciences of Ukraine, Kyiv;O.O. Bogomoletz Institute of Physiology, NationalAcademy of Sciences of Ukraine, Kyiv



Abstract

In the present study we have investigated the correlation be- tween hyperpolarization-activated current (Ih) and menthol-ac- tivated current (ITRPM8) in rat dorsal root ganglion (DRG) neurons. We showed that Ih is present in 89% of menthol-sensitive neurons which makes its presence reliable, though not absolute, criterion for pre-selection of such neurons. Endogenous Ih recorded from different neurons exhibited variable density and activation kinetics. Based on the analysis of Ih activation kinetics we hy- pothesize that the population of hyperpolarization-activated channels in menthol-sensitive DRG neurons is mainly represented by HCN1, HCN2 and HCN3 channels. The expression of HCN4 isoform in these cells is very low.

References

  1. Accili E., Proenza C., Baruscotti M., DiFrancesco D. From funny current to HCN channels: 20 years of exci- tation // News Physiol. Sci. – 2002. – 17. – P.32–37.
  2. Babes A., Zorzon D., Reid G. A novel type of cold- sensitive neuron in rat dorsal root ganglia with rapid adaptation to cooling stimuli // Eur. J. Neurosci. – 2006. –24, № 3. – P. 691–698.
  3. Chaplan S., Guo H., Lee D et al. Neuronal hyperpolar- ization-activated pacemaker channels drive neuropathic pain // J. Neurosci. – 2003. – 23, № 4. –P.1169–1178.
  4. Chen S., Wang J., Siegelbaum S. Properties of Hyper- polarization-activated pacemaker current defined by coassembly of HCN1 and HCN2 subunits and basal modulation by cyclic nucleotide // J. Gen. Physiol. – 2001. – 117, № 5. – P.491–504.
  5. de la Pena E., Malkia A., Cabedo H., Belmonte C., Viana F. The contribution of TRPM8 channels to cold sensing in mammalian neurones // J. Physiol. – 2005. – 567, № 2. – P. 415–426.
  6. Dhaka A., Viswanath V., Patapoutian A. Trp ion channels and temperature sensation // Annu. Rev. Neurosci. – 2006. – 29. – P. 135–161.
  7. DiFrancesco D. Pacemaker mechanisms in cardiac tis- sue // Annu. Rev. Physiol. – 1993. – 55. – P. 455–472.
  8. Fonfria E., Murdock P.R., Cusdin F.S. et al. Tissue dis- tribution profiles of the human TRPM cation channel family // J. Rec. Signal. Transduct. Res. – 2006. – 26, № 3. – P. 159–178.
  9. 9. Kraft R., Harteneck C. The mammalian melastatin-related transient receptor potential cation channels: an overview / / Pflug. Arch. – 2005. – 451, № 1. – P. 204–211.
  10. 10. Ludwig A., Zong X., Jeglitsch M. et al. A family of hy- perpolarization-activated mammalian cation channels // Nature. – 1998. – 11. – 393, № 6685. – P. 587–591.
  11. Maingret F., Lauritzen I., Patel A.J. et al. TREK-1 is a heat-activated background K(+) channel // EMBO J. – 2000. – 1. – 19, № 11. – P. 2483–2491.
  12. McKemy D.D., Neuhausser W.M., Julius D. Identifi- cation of a cold receptor reveals a general role for TRP channels in thermosensation // Nature. – 2002. – 416, № 6876. – P. 52–58.
  13. Moosmang S., Stieber J., Zong X. et al. Cellular ex- pression and functional characterization of four hyper- polarization-activated pacemaker channels in cardiac and neuronal tissues // Eur. J. Biochem. – 2001. – 268, № 6. – P.1646–1652.
  14. Pape H.C. Queer current and pacemaker: the hyperpolarization-activated cation current in neurons // Annu. Rev. Physiol. – 1996. – 58. – P. 299–327.
  15. Pedersen S.F., Owsianik G., Nilius B. TRP channels: an overview // Cell Calcium. – 2005. – 38, № 3–4. – P. 233–252.
  16. Petruska J.C., Napaporn J., Johnson R.D. et al. Subclassified acutely dissociated cells of rat DRG: histochemistry and patterns of capsaicin-, proton-, and ATP-activated currents // J. Neurophysiol. – 2000. – 84, № 5. – P. 2365–2379.
  17. Pierau F.K., Torrey P., Carpenter D. Effect of ouabain and potassium-free solution on mammalian thermosensitive afferents in vitro // Pflug. Arch. – 1975. – 29. – 359, № 4. – P. 349–356.
  18. Raid G., Babes A., Pluteanu F. A cold- and mentholactivated current in rat dorsal root ganglion neurones: properties and role in cold transduction // J. Physiol. – 2002. – 545, № 2. – P.595–614.
  19. 19. Schдfer K., Braun H.A. Modulation of periodic cold receptor activity by ouabain // Pflugers Arch. – 1990. – 417, № 1. – P. 91–99.
  20. 20. Viana F., de la Peсa E., Belmonte C. Specificity of cold thermotransduction is determined by differential ionic channel expression // Nat. Neurosci. – 2002. – 5, № 3. – P. 254–260.

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