Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2008; 54(4): 11-15


CIRCADIAN CHANGES OF THE DENSITY OF MELATONIN RECEPTORS 1A IN THE NEURONS OF THE SUPRACHIASMATIC NUCLEI OF THE RAT HYPOTHALAMUS UNDER CONDITIONS OF DIVERSE FUNCTIONAL ACTIVITY OF THE PINEAL GLAND

V.P. Pishak, R.Ye. Bulyk

    Bukovininan State Medical University, Chernivtsi, Ukraine
DOI: https://doi.org/10.15407/fz54.04.011

Abstract

An immunohistochemical study of the density of melatonin receptors 1A in the neurons of the rat suprachiasmatic nuclei with diverse functional activity of the pineal gland has been carried out. The density of melatonin receptors 1A under condi- tions of the physiological function of the pineal gland was characterized by clear-cut diurnal variations. Simultaneously, a dysfunction of the gland results in their marked disturbance. In case of a hypofunction of the pineal body the density of the structures was reliably lower than in case of hyperfunction. It has been demonstrated that in case of a suppressed activity of the pineal body the maximum number of melatonin receptors 1A in the neurons of the hypothalamic suprachiasmatic nuclei shifts from 02.00 a.m. to 02.00 p.m. and constitutes 0,35±0,012 conventional units (c.u.) of density, whereas a larger index is noticed at 20 hours making up 0,43±0,015 c.u. of density when the gland is activated.

Keywords: MELATONIN RECEPTORS 1A, NEURONS, SUPRACHIASMATIC NUCLEI, HYPOTHALAMUS, PINEAL GLAND

References

  1. Anisimov V.N. Melatonin: perspektivi primeneniya dlya profilaktiki raka i prezhdevremennogo stareniya . Vest. vosstanov. meditsini. - 2007. - N1 (19). - S.4-7.
  2. Bondarenko L.O. Znachennya vzaєmodії faktorіv vnutrіshn'ogo і zovnіshn'ogo seredovischa v regulyatsії funktsіonal'noї aktivnostі pineal'noї zalozi: avtoref. dis. … d-ra bіol. nauk. - K., 2003. - 36 s.
  3. Bulik R.Є. TSirkadіannі zmіni melatonіnovih retseptorіv 1a u suprahіazmatichnih yadrah gіpotalamusa . Іntegrativna antropologіya. - 2007. - N2. - S.22-24.
  4. Voronkov a.E., Ivanov a.I., Baskin I.I. i dr. Izuchenie mehanizma svya­zivaniya ligandov melatoninovih retseptorov cheloveka metodom moleku- lyarnogo modelirovaniya . Dokl. RaN. - 2005. - 403, N3. - S.409-413.
  5.  
  6. Zamorskii I.I., Pishak V.P. Funktsional'naya organizatsiya fotoperiodicheskoi sistemi golovnogo mozga . Uspehi fiziol. nauk. - 2003. - 34, N4. - S.37-53.
  7.  
  8. Komarov F.I., Rapoport S.I. Hronobiologiya i hronomeditsina. - M.: Triada-H, 2000. - 488 s.
  9. Smirnov A.N. Yadernie retseptori melatonina . SCHіl'nіst' melatonіnovih retseptorіv 1a u neironah suprahіazmatichnogo yadra schura o 02.00 za umov gіpofunktsії (a) ta gіperfunktsії (b) shishkopodіbnoї zalozi. Іmunogіstohіmіchna metodika z polіklonal'nimi antitіlami do melatonіnovih retseptorіv 1a ta streptavіdinbіotinovoyu sistemoyu vіzualіzatsії LSAB2 (peroksidazna mіtka ta dіamіnobenzidin). Dofarbovuvannya klіtinnih yader gematoksilіnom Maiera. Ob.40h, Ok.10h ab Dobovі zmіni schіl'nostі melatonіnovih retseptorіv. ISSN 0201-8489 Fіzіol. zhurn., 2008, T. 54, N 4 15 Biohimiya. - 2001. - 66, N1. - S.28-36.
  10.  
  11. Arendt J. Melatonin: characteristics, concerns, and pros-pects . J. Biol. Rhythms. - 2005. - 20. - P.291-303.
  12.  
  13. Dubocovich M.L, Markowska M. Functional MT1 and MT2 melatonin receptors in mammals . Endocrine. - 2005. - 2. - P.101-110.
  14.  
  15. Golombek D.A., Ferreyra G.A., Katz M.E. et al. Neurochemistry of mammalian entrainment: Signal transduction pathways in the suprachiasmatic nuclei .Biol. Rhythm Res. - 2000. - 31, N1. - P.56-70.
  16.  
  17. Green C., Menaker M. Circadian rhythms. Clocks on the brain . Science. - 2003. - 301, N5631. - P.319-320.
  18.  
  19. Klante G., Secci K., Masson-Pevet M. et al. Interstrain differences in activity pattern, pineal function, and SCN melatonin receptor density of rats . Amer. J. Physiol. - 1999. - 276, N4, Pt 2. - R.R1078-R1086.
  20.  
  21. Reiter R. J. Melatonin: clinical relevance . Best Pract. Res. Clin. Endocrinol. Metab. - 2003. - 17, N 2. - P.273-285.
  22.  
  23. Witt-Enderby P., Bennett J., Jarzynka M. et al. Melato- nin receptors and their regulation: biochemical and struc- tural mechanisms . Life Sci. - 2003. - 72, N2. - P.2183-2198.
  24.  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.