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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2008; 54(2): 94-99


Enlarged Ca[sup]2+[/sup]-independent release of glutamatefrom synaptosomes under centrifuge-induced hypergravity modeling

O.A. Borisova, N.V. Krisanova.

    Ин-т биохимии им. А.В.Палладина НАН Украины, Киев


Abstract

The release of L-[14C]glutamate via Na+-dependent glutamate transporters functioned in the reverse mode was investigated in cortical synaptosomes under centrifuge-induced hyper- gravity. The protonophore carbonyl cyanide-p-trifluoro- methoxyphenyl-hydrazon (FCCP) induced increase in [Na+]i, depolarized the plasma membrane, dissipated the proton gra- dient across synaptic vesicles and mitochondrial membrane, caused a fall in both the ATP level and the ATP/ADP ratio. 35 mM KCl-stimulated L-[14C]glutamate release from synapto- somes preliminary treated with 1 µM FCCP considerably increased from 27.0±2.2 % to 35.0±2.3 % of total accumu- lated synaptosomal label after centrifuge-induced hypergravity as compared to control animals (Р<0.05). We found the competitive nontransportable glutamate transporter inhibitor DL-threo-?-benzyloxyaspartate to inhibit FCCP and high KCL-stimulated release of L-[14C]glutamate. The release would be expected to occur via plasma membrane glutamate trans- porters. Transportable inhibitor of glutamate transporters- DL-threo-beta-hydroxyaspartate (DL-THA) induced heteroexchange of L-[14C]glutamate from enlarged by FCCP cytosolic pool of the neurotransmitter. DL-THA-evoked release of L-[14C]glutamate was also increased significantly after hypergravity. Combined application of KCl, DL-THA and FCCP unmasked dramatic changes in the activity of the glutamate transporters functioning in the reverse mode after centrifuge-induced G-loading.

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