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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2007; 53(6): 3-10


Proteasome activity changes inaorta, heart tissues and blood leucocytes in modelling of cholesterol atherosclerosis

Pashevin D.A., Dosenko V.E., Byts Yu. V., Moibenko A.A.



Abstract

In experiments on modelling of cholesterol atherosclerosis in rabbits (1% cholesterol-rich diet during 2 month) it was deter- mined changes of trypsin-like (ТL), chymotrypsin-like (CHTL) and peptydilglutamilpeptidase (PGPG) proteasomal activity in tissues of aorta, heart and isolated blood leukocytes. It was shown that cholesterol-rich diet caused significant in- crease of ТL (3.2 fold, Р=0.003), PGPG (1.8 fold, Р=0.003) proteasomal activity in aorta tissues, and PGPG activity (1.8- times, Р=0.003) in myocardium. In isolated blood monocytes, the CHTL and PGPG activities were significantly increased (1.9 fold, Р=0.05 and 11.6 fold, Р=0.0001, respectively) and in PMN leucocytes the PGPG activity of proteasome was also significantly increased (1.8 fold, Р=0.031). Proteasomal activity in lymphocytes during cholesterol atherosclerosis modelling had no significant changes. The data obtained indi- cate that alimentary hypercholesterolemia induces considerable changes of proteasomal activity in cardio-vascular system and blood cells that take part in atherogenesis.

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