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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2007; 53(5): 3-13

Functional Identification of a TRPM8 coldreceptor in rat prostate epithelial cells

A.P. Kondratskyi, G.V. Sotkis, O. I. Boldyrev, K.O. Kondratska, O.P. Lyubanova, Iu.B.Dyskina, D.V. Gordienko, Ia.M. Shuba

    International Center of Molecular Physiology, NationalAcademy of Sciences of Ukraine, Kyiv;O.O. Bogomoletz Institute of Physiology, National Academyof Sciences of Ukraine, Kyiv.



Abstract

For the first time by means of electrophysiology we demon- strate functional expression of cold/menthol sensitive TRPM8 channel in secretory epithelial cells plasma membrane from rat prostate. Under whole-cell patch-clamp technique rapid cooling from 33 to 21° C of freshly isolated rat prostate epi- thelial cells elicited the significant outward rectifying membrane current with close to 0 mV reversal potential characteristic of TRPM8 activation. Extracellular application of chemical ana- log of cooling, menthol (100 µM) at 33° C evoked similar current responses. Cold- and menthol-evoked currents could be detected only in elongated epithelial cells, which in prostate slices were labeled with anti-cytokeratin 8, suggesting that they belong to the apical secretory phenotype. No such current could be detected in round apparently basal epithelial cells. We conclude that in normal rat prostate functional expression of TRPM8 is restricted to plasma membrane of luminal apical epithelial cells.

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