Effects of changesin mitochondrial permeability transition of medullary cardiovascular neuronson circulation in rats
L.N.Shapoval, L.S.Pobegailo, O.V.Dmytrenko, L.G.Stepanenko, V.F.Sagach
Bogomoletz Institute of Physiology, Kyiv, Ukraine.
![](/images/cite.png)
Abstract
In acute experiments on anaesthetized with urethane
normotensive rats we studied effects of modulating the mito-
chondrial permeability transition (MPT) of the neurons in
the medullary cardiovascular nuclei – nucleus of the tractus
solitarius (NTS), paramedian reticular nucleus (PMn),
n.ambiguus (AMB), and lateral reticular nucleus (LRN) on
the systemic arterial pressure level (SAP). An increase in the
MPT with injections of an inductor for MPT phenylarsine
oxide (10 –12 М - 10 –8 М) into the medullary nuclei under
exploration has been shown to induce the lowering in the
SAP level in a dose-dependent manner. A decrease in the
MPT of the medullary neurons with either cyclosporine A or
melatonin (10 –12 М - 10 –10 М) resulted in hypertensive
responses of the SAP. Effects of phenylarsine oxide injec-
tions into the medullary nuclei were attenuated after prelimi-
nary intravenous administration of L-arginine. The data ob-
tained give evidence that functional activity of the medullary
cardiovascular neurons and their effects depend to a large
extent on the functional state of their mitochondria.
References
- Владимиров Ю.В. Нарушение барьерных свойств внутренней и наружной мембран митохондрий,некроз и апоптоз// Биол. мембраны. – 2002. – 19, №5. – С.356–377.
- Сагач В.Ф., Шиманська Т.В., Надточій С.М. Попередження постреперфузійних порушень функції серця та неефективного використання кисню за допомогою інгібіторів мітохондріальної пори// Фізіол. журн. – 2002. – 48, № 6. – С. 3–9.
- Ходоров Б.И., Сторожевых Т.П., Сурин А.М. и др. Митохондриальная деполяризация играет доми- нирующую роль в механизме нарушения нейро- нального кальциевого гомеостаза, вызванного глутаматом// Биол. мембраны. – 2001. – 18, № 6. –С. 421–432.
- Шаповал Л.Н., Сагач В.Ф., Побегайло Л.С.и др. Участие оксида азота в медуллярном контроле функции кровообращения у нормотензивных крыс/ / Нейрофизиология. – 2002. – 34, № 4. – С. 294–302.
- Andrabi S.A., Sayeed I., Siemen D. et al. Direct inhibi- tion of the mitochondrial permeability transition pore: a possible mechanism responsible for apoptotic ef- fects of melatonin// FASEB J. – 2004. – 18, №7. – P. 869–871.
- Aydogdu N., Erbas N., Atmaca G., Erten O., Kaymak K. Melatonin reduces nitric oxide via increasing arginase in rhabdomyolysis-induced acute renal failure in rats// Ren.Fail. –2006. – 28, № 5. – P. 435–440.
- Balakirev M.Y., Khramtsov V.V., Zimmer G. et al. Modulation of the mitochondrial permeability transi- tion by nitric oxide// Eur. J.Biochem. –1997. – 246, №3. – P. 710–718.
- Bernardi P. Mitochondrial transport of cations: channels, exchangers and permeability transition// Physiol.Rev. – 1999. – 79. – P. 1127–1135.
- 9. Brown G.C. Regulation of mitochondrial respiration by nitric oxide inhibition of cytochrome c oxydase// Biochem. and Biophys. Acta. – 2001. – 1504, № 1. –P. 46–57.
- 10. Brown G.C., Borutaite V. Nitric oxide inhibition of mito- chondrial respiration and its role in cell death// Free Radic.Biol.Med. – 2002. – 33, №11. – Р. 1440–1450.
- Brookes P.S.. Mitochondrial nitric oxide synthase// Mitochondrion. – 2004. – 3, №4. – Р. 187–204.
- Brookes P.S., Salinas E.P., Darley K. et al. Concentration- dependent effects of nitric oxide on mitochondrial per- meability transition and cytochrome c release// J. Biol.Chem. – 2000. – 275, №27. – P. 20474–20479.
- Cassarino D.S., Bennett J.P. An evaluation of the role of mitochondria in neurodegenerative diseases: mito- chondrial mutations and oxydative pathology, protec-tive nuclear responses, and cell death in neurode-generation// Brain Res.Rev. – 1999. – 29, №1. – Р. 1–25.
- Chowdhary S., Townend J.N. Role of nitric oxide in the regulation of cardiovascular autonomic control//
- 42ISSN 0201-8489 Фізіол. журн., 2006, Т. 52, № 6 Clin. Sci. – 1999. – 97. – Р. 5–17.
- Crompton M., Barksby E., N.Johnson, M.Capano. Mi- tochondrial intermembrane junctional complexes and their involvement in cell death// Biochimie. – 2002. – 84, № 2–3. – Р. 143–152.
- Duchen M.R. Mitochondria in health an disease: per- spectives on a new mitochondrial biology//Mol. As- pects Med. – 2004. – 25, №4. – Р. 365–451.
- Gai W.P., Messenger J.P., Yu Y.H. et al. Nitric oxide synthesizing neuons in the central subnucleus of the nucleus tractus solitarius provide a major innervation of the rostral nucleus ambiguus in the rabbit // J.Comp.Neurol. – 1995. – 357. – Р. 348–361.
- .Garthwaite J, Bolton C.I.. Nitric oxide signalling in the central nervous system// Annu.Rev.Physiol. – 1995. – 57. – Р. 683–706.
- 19. Green L.S., Kroemer G. The pathophysiology of mito- chondrial cell death// Science. – 2004. – 305, № 5684. – Р. 626–629.
- 20. Halestrap A.P. The mitochondrial permeability transi- tion: its molecular mechanism and role in reperfusion in- jury// Biochem.Soc. Symp. – 1999. – 66. – Р. 181–203.
- Kim Y.M, Talanian Т,.Billiar T.R. Nitric oxide inhibits apoptosis by preventing increases in caspase-3-like activity via two distinct mechanisms// J.Biol.Chem. – 1997. – 272, №49. – Р. 31138–31148.
- Korge P, Goldhaber J.I., Weiss J N. Phenylarsine oxide induces mitochondrial permeability transition, hypercontracture, and cardiac cell death// Amer..J.Physiol.Heart Circ.Physiol. – 2001. – 280. – №5. –H2203–H2213.
- Krukoff T.L.. Central actions of nitric oxide in regulation of autonomic functions// Brain Res. – 1999. – 30. – Р. 52–65.
- Moncada S., Erusalimsky J.D. Does nitric oxide modu- late mitochondrial energy generation and apoptosis?// Nat.Rev.Mol.Cell.Biol. – 2002. – 3, №3. – Р. 214–220.
- Nizoli E., Clementi E., Paolucci C. et al. Mitochondrial biogenesis in mammals: the role of endogenous nitric ox- ide// Science. – 2003. – 299, № 5608. – Р. 896–899.
- Paxinos G., Watson C. The Rat Brain in Stereotazic Coordinates. New York: Acad. Press, 1982.
- Piantadosi C.A., Tatro L.G, Whorton. A.R. Nitric oxide anddifferential effects of ATP on mitochondrial permeability ransition// Nitric oxide. – 2002. – 6, №1. – Р. 45–60.
- Ravagnan L, Roumier T., Kroemer G. Mitochondria, the killer organelles and their weapons// J.Cell Physiol. – 2002. – 192 , №2. – Р. 131–137.
- 29. Sastre J., Pallardo F.V., Vina J. Mitochondrial oxida- tive stress plays a kea role in aging and apoptosis// IUBMB. Life – 2000. – 49, №5. – Р. 427–435.
- 30. Shapira A.H.V., Gu M., Taanman J.-W. Mitochondria in the ideology and pathogenesis of Parkinson’s dis- ease. – In: Neuroprotection in Parkinson’s disease,Wells Med.Lim., Wells Kent. – 1998. – Р. 177–185.
- Taimor G., Hofstaetter B., Piper H.M. Apoptosis induc- tion by nitric oxide in adult cardiomyocytes via cGMP- signalling and its impairment after simulated ischemia//Cardiovasc.Res. –2000. – 45, № 3. – 588–594.
- Takuma K., Phuagphong P., Lee E. et al. Anti-apoptotic effect of cGMP in cultured astrocytes: inhibition by cGMP – dependent protein kinase of mitochondrialpermeable transition pore // J. Biol. Chem. – 2001. –276. – 51. – P.48093–48099.
- Vincent S.R., Kimura H.. Histochemical mapping of nitric oxide synthase in the rat brain// Neuroscience. – 1992. – 46. – Р. 755–784.
- Weiss J.N., Korge P., Honda H.M., Ping P. Role of mitochondrial permeability transition in myocardial disease// Circulat. Res. –2003. – 93, №4. – P. 292–301.
- Zanzami N. , Kroemer G. The mitochondrion in apoptosis: how Pandora’s box opens// Nat. Rev. Mol. Cell. Biol. – 2001. – 2, №1. – Р. 67–71.
- Zanzinger J. Role of nitric oxide in the neural control of cardiovascular function// Cardiovascular Res. – 1999. – 43. – Р. 639–649.
- Zanzinger J., Seller H. Species differences in the dis- tribution of nitric oxide synthase in brain stem regions that regulate sympathetic activity// Brain Res. – 1997. –764. – P. 265–268.
- Zoratti M., Szabo I.,. De Marchi U. Mitochondrial permeability transitions: how many doors to the house?// Bichem.Biophys.Acta. –2005. – 1706, № 1–2. – P. 40–52.
|