Investigation ofc-fos expression and nadph-diaphorase reactivity in cervical cord and brainafter fatiguing stimulation of the neck muscles in the rat
O.V. Vlasenko, V.A. Maisky, A.V. Maznychenko, A.I. Pilyavskii, V.M. Moroz.
M.I. Pirogov National Medical University, Vinnitsa,Ukraine;O.O. Bogomoletz Institute of Physiology, National Academyof Sciences of Ukraine, Kyiv, Ukraine
Abstract
The distribution of c-fos expression as a marker of neuronal
activation and NADPH-diaphorase reactivity were examined
in the cervical spinal cord, limbic structures and hypothala-
mus in anaesthetized rats after fatigue induced by intermittent
high-rate (100 s-1) electrical stimulation of the dorsal neck
muscles (mm. trapezius and splenius). In comparison to the
control or sham-stimulated animals fatiguing stimulation in-
duced significant increase in ipsilaterally in the C1–C4 spinal
segments and the contralateral central (Ce), medial (Me) nu-
clei, paraventricular nucleus of hypothalamus (Pa) and vent-
rolateral periaqueductal gray (VLPAG). In spinal cord the high-
est mean number of Fos-immunoreactive (Fos-ir) neurons per
section was found in layers 1, 4 and 5 (5.8 ± 0.9, 13.1 ± 0.9 and
11.1 ± 0.7, P < 0.05) of the dorsal horn. The order of intensity
of c-fos expression in different regions of the brain was as
following sequence: Pa > VLPAG > Ce (447.7 ± 23.5, 196.3 ±
12.7, 104.6 ± 12.3, respectively). About 50 % of double-
labeled (Fos-ir and NADPH-diaphorase reactive) cells were
found in Pa nucleus. Received data show that limbic struc-
tures, hypothalamus and VLPAG are involved in activation
after neck muscle fatigue and might contribute to nociceptive
processing and generation of the autonomic and affective
components of the muscle pain.
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