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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2006; 52(6): 3-14


Investigation ofc-fos expression and nadph-diaphorase reactivity in cervical cord and brainafter fatiguing stimulation of the neck muscles in the rat

O.V. Vlasenko, V.A. Maisky, A.V. Maznychenko, A.I. Pilyavskii, V.M. Moroz.

    M.I. Pirogov National Medical University, Vinnitsa,Ukraine;O.O. Bogomoletz Institute of Physiology, National Academyof Sciences of Ukraine, Kyiv, Ukraine


Abstract

The distribution of c-fos expression as a marker of neuronal activation and NADPH-diaphorase reactivity were examined in the cervical spinal cord, limbic structures and hypothala- mus in anaesthetized rats after fatigue induced by intermittent high-rate (100 s-1) electrical stimulation of the dorsal neck muscles (mm. trapezius and splenius). In comparison to the control or sham-stimulated animals fatiguing stimulation in- duced significant increase in ipsilaterally in the C1–C4 spinal segments and the contralateral central (Ce), medial (Me) nu- clei, paraventricular nucleus of hypothalamus (Pa) and vent- rolateral periaqueductal gray (VLPAG). In spinal cord the high- est mean number of Fos-immunoreactive (Fos-ir) neurons per section was found in layers 1, 4 and 5 (5.8 ± 0.9, 13.1 ± 0.9 and 11.1 ± 0.7, P < 0.05) of the dorsal horn. The order of intensity of c-fos expression in different regions of the brain was as following sequence: Pa > VLPAG > Ce (447.7 ± 23.5, 196.3 ± 12.7, 104.6 ± 12.3, respectively). About 50 % of double- labeled (Fos-ir and NADPH-diaphorase reactive) cells were found in Pa nucleus. Received data show that limbic struc- tures, hypothalamus and VLPAG are involved in activation after neck muscle fatigue and might contribute to nociceptive processing and generation of the autonomic and affective components of the muscle pain.

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