Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2006; 52(5): 12-20

The effectsof ?-linolenic acid on the functioning isolated heart during ischemia/reperfusion

Т.V. Кuкоbа, А. М. Shysh, А.А. Моybеnkо, А.V.Коtsyuruba, О.V.Kcharchenko

    О.О.Bogomoletz Institute of Physiology, National Academyof Sciences of Ukraine, Kyiv.Institute of Bioogranic Chemistry and Petrochemistry,National Academy of Sciences of Ukraine, Kyiv



Abstract

Many studies indicate that dietary omega-3 polyunsatu- rated fatty acids (PUFAs) have the cardioprotective prop- erties. But majority of experiments were carried out with using omega-3 PUFAs from marine fish oil. The purpose of this study was to determine effects of the plant-de- rived omega-3 PUFA (alpha-linolenic acid (a-LA) on pos- tischemic myocardial dysfunction, lipid peroxidation and antioxidant enzymes activity. Male Wistar rats (250-300 g) were divided into 4 groups (n = 10-12 each). In control group (1) were intact rats. The hearts from 2-nd group of animal were exposed to 20 min of global ischemia fol- lowed by 40 min reperfusion according to the Langendorff technique. The 3-rd and 4-th groups of animal received of the plant-derived oil (a-LA), which is a precursor of eicosapentaenoic acid and was used as a dietary supple- ment in dose 0.1 mg/kg per day for 4 weeks. The hearts from 4-th group of animal were also exposed to ischemia/ reperfusion. Analysis of myocardial phospholipid fatty acid content showed that consumption of the plant-de- rived б-LA for 4 weeks changes fatty acid profile through incorporation of б-LA in cell membranes. It also reduced content of omega-6 PUFAs in membrane phospholipids. In 3-rd group content of a-LA and EPA were increased by 1.5- and 3.5-times, respectively, whereas content of AA was reduced by 1.7-times. The development of ischemia/ reperfusion in 2-nd group caused increase of free AA content in heart tissue by 3.5-times, whereas in 4-th group this increase was only by 1.4-time. Ischemia/reperfusion of the isolated rat heart in 4-th group was accompanied by reduced leukotriene C4 and thromboxane B2 produc- tion in 3-times and 1.9-times, respectively in comparison to 2-nd group. The time of myocardial function recovery after ischemia (heart rate, left ventricular development pressure), was shorter compare to 2-nd group. Also in 4- th group end-diastolic pressure and coronary perfusion pressure during reperfusion period were significantly lower. Dietary omega-3 PUFAs resulted in remarkable de- crease of reperfusion arrhythmias in 4-th group (in 3.8- times) and limited the oxidative stress through decrease free radical and lipid peroxidation production. In this group of animals the activity of antioxidant enzymes (superoxide dismutase and catalase) after ischemia/reperfusion were higher than in 2-nd group. We suggest that dietary supple- ment of the plant-derived ?-LA for 4 weeks have cardio- protective effects similar to the effects of fish oil.

Full text: (PDF, in Ukrainian)

References

  1. Кейтс М. Техника липидологии. - М.: Мир, 1975. - 322 c.
  2. Королюк М.А., Иванова А.И., Майорова И.Т., Токарев В.Е. Метод определения активности каталазы // Лаб. дело - 1988. - № 1. - С. 16-19.
  3. Кукоба Т.В., Кириленко О.Є., Шиш А.М. та ін. Вплив модифікації жирнокислотного складу мембран фосфоліпідів на функціональний стан ізольованого серця при гострій ішемії-реперфузії міокарда // Фізіол. журн. - 2003. - 49, № 5. - С. 63-71.
  4. Соболева М.К., Шарапов В.И. Диагностическая и прогностическая значимость определения диеновых конъюгатов в плазме больных сепсисом // Клин. лаб. диагностика. - 1992. - № 9-10. - С. 15-18.
  5. Стальная И.Д., Гаришвили Т.Г. Метод определения малонового диальдегида с помощью тиобарбитуровой кислоты. - В кн.: Совр. методы в биохимии / Под ред. Ореховича В.Н. – М.: Медицина, 1977. – С. 66-67.
  6. Чевари С., Чаба И., Секей Й. Роль супероксиддис- мутазы в окислительных процессах клетки и метод определения ее в биологических материалах // Лаб. дело – 1985. - № 11. – С. 678-681.
  7. Albert C.M., Campos H., Stampfer M.J. et al. Bloodlevels of long-chain n-3 fatty acids and the risk of sud- den death // N. Engl. J. Med. – 2002 – 346, № 15. - P. 1113-1118.
  8. Ando K., Nagata K., Yoshida R. et al. Effect of n-3 polyunsaturated fatty acid supplementation on lipid peroxidation of rat organs // Lipids. – 2000 – 35, №4. – P. 401-407.
  9. 9. Bradford M.M. A rapid and sensitive method for the quantitative of microgram quantitives of protein utiliting the principle of protein dye binding // Anal. Biochem. - 1976. - 72, №1. - Р. 242-254.
  10. 10. Dietary docosahexaenoic acid is retroconverted in man to eicosapentaenoic acid, which can be quickly trans- formed to prostaglandin I3 // Prostaglandins. – 1987. – 34, №3. – p. 367-375.
  11. Fischer S., Weber P.C. Thromboxane (TX)A3 and pros- taglandin (PG)I3 are formed in man after dietary eicosapentaenoic acid: identification and quantification by capillary gas chromatography-electron impact mass spectrometry // Biomed. Mass. Spectrom. - 1985 - 12, №9. – p. 470-476.
  12. Fontani G., Corradeschi F., Felici A. et al. Cognitive and physiological effects of Omega-3 polyunsaturated fatty acid supplementation in healthy subjects // Eur. J. Clin. Invest. – 2005. – 35, № 11. – p. 691-699.
  13. Frenoux J.M., Prost E.D., Belleville J.L., Prost J.L. A polyunsaturated fatty acid diet lowers blood pressure and improves antioxidant status in spontaneously hy- pertensive rats // J. Nutr. - 2001. - 131, № 1. - P. 39-45.
  14. Holub B.J. Clinical nutrition: 4. Omega-3 fatty acids in cardiovascular care // CMAJ. – 2002. – 166, № 5. – P. 608-615.
  15. Hu F.B., Bronner L., Willett W.C. et al. Fish and omega- 3 fatty acid intake and risk of coronary heart disease in women // JAMA. - 2002. - 287, №14. – P. 1815-1821.
  16. Hu F.B., Manson J.E., Willett W.C. Types of Dietary Fat and Risk of Coronary Heart Disease // J. Amer. Coll. Nutr. – 2001 - 20, № 1. – P. 5–19.
  17. Kinoshita I., Itoh K., Nishida-Nakai M. et al. Antiar- rhythmic effects of eicosapentaenoic acid during myo- cardial infarction-enhanced cardiac microsomal (Ca(2+)-Mg2+)-ATPase activity // Jаp. Circulat. J. – 1994 – 58, №12. – P. 903-912.
  18. Korpela R., Seppo L., Laakso J. at al. Dietary habits affect the susceptibility of low-density lipoprotein to oxidation // Eur. J. Clin. Nutr. - 1999. - 53, № 10. - P. 802-807.
  19. 19. Kromhout D., Feskens E.J.M., Bowles C.H. The pro- tective effect of a small amount of fish on coronary heart disease mortality in an elderly population // Int. J. Epidemiol. - 1995. - 24, № 2. - p. 340–345.
  20. 20. Mori T.A., Woodman R.J., Burke V. et al. Effect of eicosapentaenoic acid and docosahexaenoic acid on oxidative stress and inflammatory markers in treated- hypertensive type 2 diabetic subjects // Free Radic. Biol. Med. – 2003. – 35, №7. – Р. 772-781.
  21. Nair S.S., Leitch J.W., Falconer J., Garg M.L. Preven- tion of cardiac arrhythmia by dietary (n-3) polyun- saturated fatty acids and their mechanism of action // J. Nutr. - 1997. - 127, №3. – P. 383-393.
  22. Oudot F., Cordelet C., Sergiel J.P., Grynberg A. Poly- unsaturated fatty acids influence prostanoid synthe- sis in vascular endothelial cells under hypoxia and re- oxygenation // Int. J. Vitam. Nutr. Res. - 1998. - 68, № 4. - P. 263-271.
  23. Rupp H., Wagner D., Rupp T. et al. Risk stratification by the «EPA+DHA level» and the «EPA/AA ratio» focus on anti-inflammatory and antiarrhythmogenic effects of long-chain omega-3 fatty acids // Herz. - 2004 – 29, №7. – Р. 673-685.
  24. Simopoulos A.P. Essential fatty acids in health and chronic disease // Amer. J. Clin. Nutr. - 1999. - 70, Suppl. 3. - P. 560S–569S.
  25. Smith W.L. Cyclooxygenases, peroxide tone and the allure of fish oil // Curr. Opin. Cell. Biol. - 2005 – 17, №2. – Р. 174-182.
  26. Song J.Y., Fujimoto K., Miyazawa T. Polyunsaturated (n-3) fatty acids susceptible to peroxidation are in- creased in plasma and tissue lipids of rats fed docosahexaenoic acid–containing oils // J. Nutr. - 2000. – 130, №12. - P. 3028–3033.
  27. Strasser T., Fischer S., Weber P.C. Inhibition of leukotriene B4 formation in human neutrophils after oral nafazatrom (Bay g 6575) // Biochem. Pharmacol. – 1985. – 34, №11. - P. 1891-1894.
  28. Takahashi Y., Reddy G.R., Veda N. Et al. Arachidonate 12-lipoxygenase of platelet-type in human epidermal cells // J. Biochem. – 993. – 268, №22. - Р. 16443-16448.
  29. 29. Vidgren H.M., Agren J.J., Schwab U. at al. Incorpora- tion of n-3 fatty acids into plasma lipid fractions, and erythrocyte membranes and platelets during dietary supplementation with fish, fish oil, and docosahexae- noic acid-rich oil among healthy young men // Lipids. - 1997. - 32, № 7. - P. 697-705.
  30. 30. Yang P., Chan D., Felix E. et al. Formation and antiproliferative effect of prostaglandin E(3) from eicosapentaenoic acid in human lung cancer cells // J. Lipid Res. – 2004 – 45, №6. – Р. 1030-1039.
  31. Young G.S., Conguer J.A., Thomas R. Effect of randomized supplementation with high dose olive, flax or fish oil on serum phospholipid fatty acid levels in adults with attention deficit hyperactivity disorder // Reprod. Nutr. Dev. - 2005 – 45, №5. – Р. 549-558.
  32. Yuan Y.V., Kitts D.D. Dietary (n-3) fat and cholesterol alter tissue antioxidant enzymes and susceptibility to oxidation in SHR and WKY Rats // J. Nutr. - 2003. –133, №3. - P. 679–688.
© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.