Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2006; 52(1): 101-105

Investigation of macromolecules permeability through the mouth cavitymucous on hamster’s cheek pouches

P.L. Starokadomskyy

    Institute of Molecular Biology and Genetics, NationalAcademy of Sciences of Ukraine, Kyiv, Ukraine



Abstract

The technique of permeability assessment for high-molecular compounds is presented in present work. Isolated hamster cheek pouches as a simple and convenient model for the trans- port studies were used. It was shown that a mucous membrane is permeable for ?-interferon, insulin but not for nucleic acids and peroxidase. Buccal permeability for insulin is provided by its transport through the intercellular ways. Data obtained in experiments with use of rodent keratinized mucous membrane could not be directly extrapolated to human buccal mucous permeability due to significant differences in the nature and organization of intercellular lipids in these species. However, we supposed that freshly isolated hamster cheek pouch could be employed as initial step of assessment for the fast screen- ing of buccal permeability for the high-molecular compounds.

Full text: (PDF, in Ukrainian)

References

  1. Генесина Т.И. Исследование проницаемости слизис- той защечних мешков хомяков для электролитов и неэлектролитов: Автореф.дис. … канд.биол.наук. –Одесса, 1971. – 22 с.
  2. Справочник биохимика: Пер. с англ. – М.: Мир,1991. – 544 с.
  3. Старокадомский П. Л., Дубей И. Я. Лизальбиновая кислота – новый стимулятор всасывания для буккального введения белковых препаратов // Укр. биохим. журн. – 2005 – 77, №6. – С. 78–82.
  4. Старокадомский П.Л., Скиба О.И. Проницаемость слизистой оболочки полоти рта для некоторых неорганических и органических молекул // Вісн. стоматології. – 2003. – №3. – С. 2–9.
  5. Фримель Х. Иммунологические методы. – М.: Мир, 1979. – 518 с.
  6. al-Achi A., Greenwood R. Buccal administration of human nsulin in streptozocin-diabetic rats // Res. Commun. Chem.Pathol. Pharmacol. – 1993. – 82, №3. – P. 297–306.
  7. Bayley D., Temple C., Clay V. et al. The transmucosal absorption of recombinant human interferon-alpha B/ D hybrid in the rat and rabbit // J. Pharm. Pharmacol. –995. – 47, № 9. – P. 721–724.
  8. Hubbard R.C., Casolaro M.A., Mitchell M. et al. Fate of aerosolized recombinant DNA-produced alpha 1- antitrypsin: use of the epithelial surface of the lower respiratory tract to administer proteins of therapeutic importance // Proc. Natl. Acad. Sci. USA. – 1989. – 86,№2. – P. 680–684.
  9. 9. Jacobsen J. TR146 cells grown on filters as a model for human buccal epithelium: I. Morphology, growth, bar- rier properties, and permeability // Int. J. Pharm. –1995. – 125. – P. 165–184.
  10. 10. Kataoka M., Masaoka Y., Yamazaki Y. et al. In vitro system to evaluate oral absorption of poorly water- soluble drugs: simultaneous analysis on dissolution and permeation of drugs // Pharm Res. – 2003. – 20, №10. –P. 1674–1680.
  11. Laemmli U.K. Cleavage of structural proteins during the assembly of the head of bacteriophage T4 // Na- ture. – 1970. – 15, 227. – P. 680–685.
  12. Senel S., Kremer M., Nagy K. et al. Delivery of bioactive peptides and proteins across oral (buccal) mucosa // Cur. Pharm. Biotech. – 2001. – № 2. –P. 175–186.
  13. Squier C.A., Eady R.A., Hopps R.M. The permeabil- ity of epidermis lacking normal membrane-coating gran- ules: an ultrastructural tracer study of Kyrle-Flegeldisease // J. Invest. Dermatol. – 1978. – 70, №6. –P. 361–364.
  14. Tian-Zhi Y., Xiang-Tao W., Xue-Ying Y. et al. Phos- pholipid Deformable Vesicles for Buccal Delivery of Insulin // Chem. Pharm. Bull. – 2002. – 50, №6. – P.749–753.
  15. Xu H.B., Huang K.X., Zhu Y.S. et al. Hypoglycaemic effect of a novel insulin buccal formulation on rabbits // Pharmacol Res. – 2002. – 46, №5. – P. 459–467.
© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.