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Investigation of macromolecules permeability through the mouth cavitymucous on hamster’s cheek pouches
P.L. Starokadomskyy
Institute of Molecular Biology and Genetics, NationalAcademy of Sciences of Ukraine, Kyiv, Ukraine
Abstract
The technique of permeability assessment for high-molecular
compounds is presented in present work. Isolated hamster
cheek pouches as a simple and convenient model for the trans-
port studies were used. It was shown that a mucous membrane
is permeable for ?-interferon, insulin but not for nucleic acids
and peroxidase. Buccal permeability for insulin is provided by
its transport through the intercellular ways. Data obtained in
experiments with use of rodent keratinized mucous membrane
could not be directly extrapolated to human buccal mucous
permeability due to significant differences in the nature and
organization of intercellular lipids in these species. However,
we supposed that freshly isolated hamster cheek pouch could
be employed as initial step of assessment for the fast screen-
ing of buccal permeability for the high-molecular compounds.
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© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.
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