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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2006; 52(1): 30-40

Characteristics ofinositoltrisphosphate-sensitive Cа2+ stores in the acinar cells of rat submandibularsalivary gland

О.V. Коpach, І.А. Kruglikov, P.G. Kostyuk, N.V. Voitenko, N.V. Fedirko

    Ivan Franko Lviv National University, LvivО.О. Bogomolets Institute of Physiology, National Academyof Sciences of Ukraine, Kyiv



Abstract

In the acinar cells of rat submandibular salivary gland activa- tion of cholinoreceptors leads to the release of Ca2+ from endo- plasmic reticulum (ER). This Ca2+ release from ER is mainly mediated by ІnsP3-receptors. In the present work we used Arsenazo III dye and mag-fura 2/AM to measure total cellular calcium content and Ca2+ concentration in the ER ([Ca2+]ER), respectively. We have found that application of ІnsP3 to the permeabilized acinar cells evoked decrease [Ca2+]ER in dose- dependent manner with EC50 1,3 ± 0,21 mM. This ІnsP3- induced Ca2+ release from the ER was potentiated by Са2+ in the physiological ranges (100-400 nM), modulated by caffeine and ATP. Low concentrations of ATP in (<1 mM) enhanced the ІnsP3-induced decrease [Ca2+]ER while high concentrations of ATP markedly suppressed Ca2+ release. Caffeine (2 mM) decreased ІnsP3-induced Ca2+ release in the presence of Ca2+ however it has no inhibitory effect in the absence of Ca2+. This inhibitory effect of caffeine on ІnsP3-induced Ca2+ release is overcame by high concentration of InsP3 (20 mM) and ATP (1 mM) indicating that caffeine functionally competes with InsP3 Властивості інозитолтрифосфатчутливого депо Са2+ ISSN 0201-8489 Фізіол. журн., 2006, Т. 52, № 1 39 receptor domains. We suggested that the ATP regulation of InsP3-induced Ca2+ release might also play a role in oscilla- tions of intracellular Ca2+ and the maintenance of the cell sur- vival during energy attenuation periods

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