Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2006; 52(1): 8-16

Mechanisms of infrigement ofcontractive reactions and efficiency of oxygen utilisation by portal vein smooth musclesat experimental diabetes mellitus

O.D. Prysyazhna, A.V.Kotsuruba, M.N. Tkachenko, V.F. Sagach

    О.О. Bogomolets Institute of Physiology, National Academyof Sciences of Ukraine, KyivA.V. Palladin Institute of Biochemistry National Academy ofSciences of Ukraine, Kyiv



Abstract

On the rat portal vein isolated strips the essential reduction of contractive response, increase of stiffness of vascular smooth muscles at their stretching and increase of oxygen value of smooth muscles’ work are revealed in conditions of experi- mental diabetes mellitus. The possible mechanism of such infringements is chronic hyperglycemia, which results in de- velopment of oxidative stress and reduction of NO synthesis by constitutive NOS. Indeed, the significant decrease of activ- ity of constitutive NO-synthase and increase of the oxidative stress markers contents are shown at experimental diabetes mellitus. The similar infringements are marked at in vitro model of hyperglycemia, blockade of NO-synthase and in conditions of oxidative stress. Additional confirmation of a role of NO synthesis blockade and oxidative stress in development of such infringements at diabetes is fact that application of anti- oxidant melatonin and L-arginine, a precursor of NO to dia- betic animals results in partial restoration of contractive reactions, reduction of stiffness and oxygen value of smooth muscles’ work .

Full text: (PDF, in Ukrainian)

References

  1. Гаврилов В.Б., Гаврилова А.Р., Хмара Н.Ф.Измерение диеновых конъюгатов в плазме крови по УФ-поглощению гептановых и изопропанольных экстрактов // Лаб. дело. – 1988. – № 2. – С. 60–64.
  2. Березовский В.А. Напряжение кислорода в тканях животных и человека. – К.: Наук. думка, 1975. – 277 с.
  3. Джавадов С.А., Джохаридзе Т.З., Джалиашвили И.В. и др. Исследование энергетического метаболизма и сократительной функции сердца с диабетической кардиомиопатией: действие ишемии и реперфузии //Біохімія. – 1992. – 57, № 12. – С. 1917–1929.
  4. Коваленко Е.А., Березовский В.А., Эпштейн И.М. Полярографическое определение кислорода в организме. – М.: Медицина. 1975. – 230 с.
  5. Сагач В.Ф., Ткаченко М.Н. Роль эндотелия во взаимо- отношениях длина-сила сосудистых гладких мышц // Докл. АН Украины – 1993. – № 10. – С. 168–172.
  6. Сагач В.Ф., Ткаченко М.М., Присяжна О.Д. та ін. Зміни вазодилататорних реакцій судинних гладеньких м’язів та системи оксиду азоту за умов експериментального цукрового діабету // Фізіол. журн. –2003. – 49, № 4. – С. 24–32.
  7. Сагач В.Ф., Ткаченко М.М., Шаповал М.В. Роль зміни системи оксиду азоту в порушеннях скорочувальних реакцій судинних гладеньких м’язів при старінні // Доп.НАН України – 2000. – № 12. – С. 194–198.
  8. Ткаченко М.М. Скорочувальні реакції судинних гладеньких м’язів за умов гіперхолестеринемії та змін функціональної активності ендотелію // Фізіол.журн. – 1997. – 43, №1–2. – С. 57–63.
  9. 9. Ткаченко М.М. Роль ендотеліну в скорочувальних реакціях судинних гладеньких м’язів гіпертензивних щурів // Там само. – № 5–6. – С. 89–95.
  10. 10. Хаютин В.М., Рогоза А.И. Регуляция кровеносных сосудов, порождаемая приложенными к ним механическими силами. – В кн.: Руководство по физиологии. Физиология кровообращения. Регуля-ция кровообращения. – Л.: Наука, 1986. – С. 37–66.
  11. Bruckdorfer R. The basics about nitric oxide // Mol. Aspects Med. – 2005. – 26, № 1–2. – P. 3–31.
  12. Brown B.P., Heistad D.D. Capacitance of the rabbit portal vein and inferior vena cava // J. Physiol. – 1986. –№ 381. – P. 417–425.
  13. Brownlee M. Biochemistry and molecular cell biology of diabetic complications // Nature. – 2001. – 414, № 6865. – P. 813–820.
  14. Calles-Escandon J., Cipolla M. Diabetes and Endothe-lial Dysfunction: A Clinical Perspective // Endocr. Rev. –2001. – 22, №1. – P. 36–52.
  15. Ceriello A. New Insights on Oxidative Stress and Diabetic Complications May Lead to a «Causal» Antioxidant Therapy // Diabetes. Care. – 2003. – 26, №5. –P. 1589–1596.
  16. Clarkson P., Celermajer D.S., Donald A.E. еt аl. Impaired vascular reactivity in insulin-dependent diabetes mellitus is related to disease duration and low density lipoprotein cholesterol levels // J. Amer. Coll.Cardiol. – 1996. – 28, №3. – P. 573–579.
  17. Creager M.A., Luscher T.F., Cosentino F., Beckman J.A. Diabetes and Vascular Disease: Pathophysiology,Clinical Consequences, and Medical Therapy: Part I //Circulation – 2003. – 108, №12. – P. 1527–1532.
  18. Eren M., Gorgulu S., Uslu N. et al. Relation between aortic stiffness and left ventricular diastolic function in patients with hypertension, diabetes, or both //Heart. – 2004. – 90, №1. – P. 37–43.
  19. 19. Loke K.E., McConnell P.I., Tuzman J.M. et al. Endog- enous Endothelial Nitric Oxide Synthase-Derived Nitric Oxide Is a Physiological Regulator of Myocardial Oxygen Consumption // Circulat. Res. – 1999. – 84,№7. – P. 840–845.
  20. 20. Mellander S., Johansson B. Control of resistance, exchange, and capacitance functions in the peripheral circulation // Pharmacol. Rev. – 1968. – 20, №3. – P. 117–196.
  21. Neely J.R., Liebermeister H., Battersby E.J., Morgan H.E. Effect of pressure development on oxygen consumption by isolated rat heart // Amer. J. Physiol. –О.Д. Присяжна, А.В. Коцюруба, М.М. Ткаченко, В.Ф. Сагач
  22. 61ISSN 0201-8489 Фізіол. журн., 2006, Т. 52, № 11967. – 212, №4. – P. 804–814.
  23. Oxlund H., Rasmussen L.M., Andreassen T.T.,Heickendorff L. Increased aortic stiffness in patientswith type 1 (insulin-dependent) diabetes mellitus //Diabetologia – 1989. – 32, №10. – P. 748–752.
  24. Prysyazhna O.D., Sagach V.F. The influence of oxida-tive stress inductor tert-butyl hydroperoxide on contractive reactions of aorta smooth muscles // IASN 2004, Crimea Ibro advanced school of neuroscience (abstract book) – 2004. – P. 38.
  25. Rodriguez-Manas L., Lopez-origa P., Petidier R. et al. Effect of glycaemic control on the vascular nitric oxide system in patients with type 1 diabetes // J. Hyper- tension. – 2003. – 21, № 6. – P. 1137–1143.
  26. Sims T.J., Rasmussen L.M., Oxlund H., Bailey A.J. The role of glycation cross-links in diabetic vascular stiffening // Diabetologia – 1996. – 39, №8. – P. 946–951.
  27. The diabetes control and complications trial research group. The effect of intensive treatment of diabetes on the development and progression of long-term complications in insulin-dependent diabetes mellitus //N. Engl. J. Med. – 1993. – 329, №14. – P. 977–986.
  28. Trochu J.N., Bouhour J.B., Kaley G., Hintze T.H. Role of endothelium-derived nitric oxide in the regulation of cardiac oxygen metabolism : Implications in health and disease // Circulat. Res. – 2000. – 87,№12. – P. 1108–1117.
  29. Tuck M L. Nitric oxide in diabetes mellitus // J. Hy-pertension. – 2003. – 21, №6. – P. 1081–1083.
  30. 29. Williams S.B., Goldfine A.B., Timimi F.K. et al. Acute hyperglycemia attenuates endothelium–dependent va-sodilation in humans in vivo // Circulation. – 1998. –97, №17. – P. 1695–1701.
  31. 30. Winer N., Sowers J.R. Vascular compliance in diabetes // Curr. Diab. Rep. – 2003. – 3, №3. – P. 230–234.
  32. Zhao G., Bernstein R.D., Hintze T.H. Nitric oxide and oxygen utilization: exercise, heart failure and diabetes // Coron. Artery. Dis. – 1999. – 10, №5. – P. 315–320.
  33. Zhao G., Zhang X., Xu X. et al. Depressed modulation of oxygen consumption by endogenous nitric oxide in cardiac muscle from diabetic dogs // Amer. J. Physiol.Heart. Circ. Physiol. – 2000. – 279, № 2. – P. H520–H527.
© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.