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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2006; 52(1): 8-16


Mechanisms of infrigement ofcontractive reactions and efficiency of oxygen utilisation by portal vein smooth musclesat experimental diabetes mellitus

O.D. Prysyazhna, A.V.Kotsuruba, M.N. Tkachenko, V.F. Sagach

    О.О. Bogomolets Institute of Physiology, National Academyof Sciences of Ukraine, KyivA.V. Palladin Institute of Biochemistry National Academy ofSciences of Ukraine, Kyiv


Abstract

On the rat portal vein isolated strips the essential reduction of contractive response, increase of stiffness of vascular smooth muscles at their stretching and increase of oxygen value of smooth muscles’ work are revealed in conditions of experi- mental diabetes mellitus. The possible mechanism of such infringements is chronic hyperglycemia, which results in de- velopment of oxidative stress and reduction of NO synthesis by constitutive NOS. Indeed, the significant decrease of activ- ity of constitutive NO-synthase and increase of the oxidative stress markers contents are shown at experimental diabetes mellitus. The similar infringements are marked at in vitro model of hyperglycemia, blockade of NO-synthase and in conditions of oxidative stress. Additional confirmation of a role of NO synthesis blockade and oxidative stress in development of such infringements at diabetes is fact that application of anti- oxidant melatonin and L-arginine, a precursor of NO to dia- betic animals results in partial restoration of contractive reactions, reduction of stiffness and oxygen value of smooth muscles’ work .

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