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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2006; 52(1): 3-7


Proteasomal degradation of RNA of differentNO-synthase isoforms

V.E. Dosenko, V.Yu. Zagoriy, A.A. Moibenko

    О.О. Bogomolets Institute of Physiology, National Academyof Sciences of Ukraine, Kyiv


Abstract

Using a developed method of determination of the RNase activity of the proteasome in vitro with the application of reverse transcription followed by subsequent polymerase chain reaction it was shown that 26S proteasome from the proteasomal fraction II effectively cleaves RNA, encoding ac- tin, myosin and all isoforms of NO synthase. The intensity of RNA degradation by proteasome and specific RNases is simi- lar. It was also shown that clasto-lactacystin ?-lactone, a spe- cific proteasome inhibitor significantly depresses RNase ac- tivity of the proteasome. Thus, proteasome is capable to de- grade certain eukariotic RNA in vitro and the proposed method can be used in order to discover specific substances such as inhibitors of RNase activity of the proteasome.

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