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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2005; 51(3): 52-56

Influence of oxygen and nitrogenactive compounds on ph value inuterus myocytes

Iu. V. Danylovych, V.A. Tugai

    A.V. Palladin Institute of Biochemistry National Academy ofScience of Ukraine, Kyiv



Abstract

In suspension from a rat uterus using fluorescent рНi probe BCECF-AM was established that the „apparent” рНі value for this object was 6,72±0,02 at рНо=7,4 (t = 25о С, n=5). Cell activation by 0,1 mM carbachol was accompanied by a rise in cytosolic рН up to 6,81±0,01. This process with different efficiency was inhibited by blockers of K+- and Н+-channels: 0,1 mM 4-amidopyridine, tetraethilamonium and cadmium. The blockers of passive Н+-transport do not inhibit completely alkalization of myoplasm that can testify in the favor of the non-channel way of passive transport Н+ through plasma- lemma. It was established that NO (0,1 mM sodium nitro- prusside) and NO2- (10 nM) significantly enhanced the carbochol-induced rise of myoplasm рН in the cells from myometrium. It also was shown that carbachol-induced рНі in myocytes was increased by the addition of 10 nM Н2О2. It was suggested that the studied compounds could influe

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