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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2005; 51(3): 3-11


Nitric oxide suppresses permeabilitytransition pore opening and enhancescalcium uptake in mitochondria in vivo

O.V. Akopova, A.V. Kotsiuruba,Yu.P. Tkachenko, V.F. Sagach

    A.A. Bogomoletz Institute of Physiology, National Academy ofScience of Ukraine, KyivA.V. Palladin Institute of Biochemistry National Academy ofScience of Ukraine, Kyiv


Abstract

The influence of an NO donor, nitroglycerine (NG) on Са2+ accumulation in rat heart and liver mitochondria was studied. A rapid dose-dependent increase in Са2+-uptake was observed within 5 min after NG injection in vivo, with a peak in NG concentration of 0,5-1,0 mg /kg body weight. There is a correlation between a dose-dependent increase in Са2+-accu- mulating capacity of mitochondria and sharp dose-dependent rise in stable NO metabolites level (NO2-, NO3- and nitrosothiols) and reactive oxygen species (Н2О2 аnd hydroxyl radical, .ОН) as well as lipid oxidation products (diene conjugates), the markers of the oxidative damage of the organelles in mitochondria in comparison to a whole tissue (liver) where only moderate increase of ROS and NO metabo- lites was detected. A 20-30-fold increase of NO3- level from 32,4±2,6 to 736,4±96,5 nmol/mg protein (p<0,05) and from 45,4±6,2 to 1395,0±121,1 nmol/mg protein (p<0,05) in liver and heart mitochondria respectively also provide a strong evi- dence for nitrosative stress. The results obtained suggest that calcium elimination from cytosol and its increased accumula- tion in mitochondria (likely dependent on permeability transi- tion pore blockade by NO) could account for the cardio- protective action of NG although resulted in the development of oxidative stress with following long-term alterations of the energy supply and metabolism of subcellular structures.

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