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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2004; 50(6): 107-113


Features of lipid droplets ultrastructurein rat adrenocortical cells from zona fasciculata-reticularis

S.L. Tokar, L.M. Koval, E.N. Yavorskaya, E.A. Lukyanetz

    Bogomoletz Institute of Physiology NAS of Ukraine, Kyiv


Abstract

One day cultured adrenocortical cells from zona fasciculatareticularis were used in morphological experiments. The electron- microscopic and imaging analysis methods were used for the investigation of intracellular ultrastructure of these cells. Experiments which conducted in control conditions, allowed us to allocate three types of cells which differed by ultrastructure of the mitochondria, lipid droplets, smooth endoplasmic reticulum and dense bodies. It was shown that lipid droplets with light and homogeneous matrix, met more often in cells with morphological attributes of high intensity of steroidogenesis. On the contrary, lipid droplets, with dark matrix and a dense edging, met more often in cells which having morphological attributes of low intensity of steroidogenesis. Ionophore A23187 or adrenocorticotropic hormone application resulted in reduction of lipid droplets diameter and in simultaneous increase in density of their arrangement in cytoplasm. At the same time droplet matrix became light and homogeneous in all cells. Thus, the ultrastructure of lipid droplet matrix is sensitive to change of calcium ions concentration in cytoplasm. Processes which result in change of lipid droplet ultrastructure, probably, are connected to steroidogenesis, nevertheless, this question requires further investigation. The lipid droplets. ability to form morphological contacts with smooth endoplasmic reticulum, mitochondria, nuclear and cellular membranes is also discussed.

References

  1. Гордиенко В.М., Козырецкий В.Г. Ультраструктура желез эндокринной системы. – К.: Здоров’я, 1978. – 137 с.
  2. Коваль Л.М., Яворская Е.Н., Лукьянец Е.А., Токарь С.Л. Ультраструктурные характеристики стероидных везикул и митохондрий из адренокор- тикальных клеток быка// Нейрофизиология/Neuro- physiology. – 2000. – 32. – С. 272–274.
  3. Токар С.Л., Коваль Л.М., Лукьянец О.О., Яворська О.М. Особливості ультраструктури культуральних клітин кори надниркових залоз щура у нормі та після аплікації кальцієвого іонофору А23187 та АКТГ// Фізіол. журн. – 2004. – 50. – С. 105–110.
  4. Andreis P.G., Rebuffat P., Belloni A.S., Neri G., Cavallini L. et al. Stereological and functional investigationson isolated adrenocortical cells: zona fasciculata/reticularis cells of chronically ACTH- treated rats// Cel Tissue Res. – 1989. – 258, №1. – P. 43–51.
  5. Bornstein S.R., Ehrhart-Bornstein M., Guse-Behling H., Scherbaum W.A. Structure and dynamics of adre- nal mitochondria following stimulation with cortico- tropin releasing hormone //Anat. Rec. – 1992. – 234, №2. – P. 255–262.
  6. Clifford G.M., Londos C., Kraemer F.B. et al. Trans- location of hormone-sensitive lipase and perilipin upon lipolytic stimulation of rat adipocytes // J. Biol. Chem. – 2000. – 275, №7. – P. 5011–5015.
  7. Hall P.F., Almahbobi G. Roles of microfilaments and intermediate filaments in adrenal steroidogenesis // Microsc. Res. Tech. – 1997. – 36, №6. – P. 463–479.
  8. Koizuka S. Electron microscopic studies on the effect of ACTH and flavin adenine dinucleotide on adreno- cortical atrophy of hypophysectomized rat // Acta Pathol Jpn. – 1977. – 27, №5. – P. 637–645.
  9. 9. Koval L.M., Yavorskaya E.N., Lukianetz E.A. Ultrrastructural features of medullary chromaffin cell cultures// Neurosciense. – 2000. – 96. – Р. 639–649.
  10. 10. Mendis-Handagama S.M. Peroxisomes and intracellu- lar cholesterol trafficking in adult rat Leydig cells following Luteinizing hormone stimulation // Tissue Cell. – 2000. – 32, № 1. – P. 102–106.
  11. Morimoto C., Kameda K., Tsujita T., Okuda H. Relationships between lipolysis induced by various lipolytic agents and hormone-sensitive lipase in rat fat cells // J. Lipid Res. – 2001. – 42. – P. 120–127.
  12. Mrotek J.J., Mathew J.K., Curtis J.C., Johansson K.R. A method for the isolation of lipid droplet fractions from decapsulated rat adrenals // Steroids. – 1981. – 38, №2. – P. 229–241.
  13. Orso E., Toth I.E., Szabo D., Szilagyi G. Changes in adrenocortical lipid fluidity of hyperfunctioning hu- man adrenals // J. Steroid Biochem. Mol. Biol. – 1992. – 41, №3–8. – P. 791–793.
  14. Ostermeyer A.G., Paci J.M., Zeng Y. et al. Accumula- tion of caveolin in the endoplasmicreticulum redirects the protein to lipid storage droplets // J. Cell. Biol. – 2001. – 152, №5. – P. 1071–1078.
  15. Pola A., Lutterforsta R., Lindsaya M. et al. A caveolin dominant negative mutant associates with lipid bodies and induces intracellular cholesterol imbalance // J.Cell. Biol. – 2001. – 152, №5. – P.1057–1070.
  16. Saltiel A.R. Another hormone-sensitive triglyceride lipase in fat cells // Proc. Nat. Acad. Sci. USA. – 2000. – 97, №2. – P. 535–537.
  17. Tauchi-Sato K., Ozeki S., Houjou H. et al. The Surface of Lipid Droplets Is a Phospholipid Monolayer with a Unique Fatty Acid Composition// J. Biol. Chem. – 2002. – 277, №46. – P. 44507–44512.
  18. Thomson M. Molecular and cellular mechanisms used in the acute phase of stimulated steroidogenesis // Horm. Metab Res. – 1998. – 30, №1. – P. 16–28.
  19. 19. Wen-Jun S., Vanita N., Shailja P. et al. Adrenal Neutral Cholesteryl Ester Hydrolase: Identification, Subcellu- lar Distribution, and Sex Differences // Endocrinology. – 2002. – 143, №3. – P. 801–806.

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