Sensitivity totonic pain and analgin in two lines of mice with a genetic knockout
Limansky Y.P., Tamarova Z.A., Limanskaja L.I., Kostyuk O.I., Mitruzaeva V.A.
A.A.Bogomoletz Institute of Physiology, National Academy ofScience of Ukraine, Kiev
Abstract
A comparative study of behavioral reactions (licking of back
paw, sleeping, eating, running, washing) in mice with a genetic
knockout (lines C57BL/6L and CBA/CaLac) and in control
animals was carried out. Experiments have been performed in
control conditions (without any influences), after a subcuta-
neous injection of 5% formalin solution, and also in a case
when animals before an injection of formalin received analgin.
It was established that the behavior of mice with a genetic
knockout without any influences statistically authentically
differs from mice without genotype infringements. Sleep du-
ration in mice with a genetic knockout was 3-4 times less, and
duration of eating was 5-13 times less than in control animals,
but grooming (washing) was more intensive (in 2,4 times).
Duration of painful reaction (licking of back paw) in response
to formalin injection in mice with a genetic knockout was in
1,3 – 2,4 times less, than in mice of control group. Analgin in a
doze of 8,3 mg/kg in genetic knockout mice suppressed pain
more poorly, than in mice of control group. Analgesia in control
group amounted 74,1%, and in genetic knockout mice it was
27,6-28,9%. The results allow concluding that mice with a
genetic knockout differ from control animals both by initial
behavior, and by reaction to a tonic pain. Analgin causes a
weaker analgesic action in mice with a genetic knockout in
comparison with animals of control group.
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