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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2004; 50(1): 31-38


Effect of sodium-calcium exchanger blockers on acetylcholine-inducedhyperpolarization of endothelial cells from excised rat aorta.

A.I. Bondarenko

    Institute of Gerontology Academy of Medical Sciences of Ukraine, Kiev


Abstract

The effect of selective sodium-calcium exchanger blockers on acetylcholine-induced hyperpolarization of endothelial cells from excised rat aorta was investigated. Acetylcholine administration induced sustained endothelial hyperpolarization. In the presence of 100 µM benzamil, the blocker of forward and reversed mode of the exchanger, acetylcholine induced only short-lived hyperpolarization. When benzamil was introduced during the plateau phase of hyperpolarization the amplitude of hyperpolarization significantly and reversibly decreased.KB-R7943 (20 µM), the blocker of reversed sodium-calciumexchanger, exerted the similar effect. Benzamil-mediated inhibitionof sustained hyperpolarization was observed when recordings were commenced from isolated endothelium. The results obtained suggest that reversed mode of sodium-calcium exchanger is activated in intact endothelial cells following stimulation by acetylcholine.

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