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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2003; 49(6): 14-21


Cardioprotective effect of heme oxigenase-1induction by hemin on the isolated heart of rat at ischemia/reperfusion

T.V. Kukoba, O.O. Moybenko, A.V. Kotsjuruba

    Bogomoletz Institute of Physiology National Academy ofSciences of Ukraine,A.V. Palladin Institute of Biochemistry National Academy ofSciences of Ukraine


Abstract

The aim of the study was to determine the role of both an inducible isoform of heme oxygenase (HO-1) and products of heme catabolism (carbon monoxide (CO), cardiac bilirubin and Fe2+) in protecting myocardium against post-ischemic myocardial dysfunction. Rat hearts were isolated and per- fused according to the Langendorff technique to evaluate the recovery of myocardial function after total ischemia (20 min) and reperfusion (40 min) and production of reactive oxygen forms at a reperfusion phase. Ischemia/reperfusion caused impairment in myocardial function: left ventricular develop- ing pressure (LVDP) was shown to be decreased , while end- diastolic pressure (EDP) and both coronary perfusion pres- sure and coronary resistance increased. Free oxygen radicals were generated at the reperfusion phase which led to injuries to cardiomyocytes and creatine kinase efflux into perfusate. We have found that upregulation of HO-1 by preliminary (24 h before ischemia) injections of 25 mg/kg hemin (i.p) resulted in improving the myocardial function due to increas- ing the enzyme activity and forming the cardial bilirubine, while generation of reactive oxygen forms was inhibited, as well as the contents of creatine kinase reduced. As a result, impairment in cardiomyocytes diminished, and coronary vessels dilated to improve the functional paramrtres of the heart work

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