Study of apoptosis manifestations intype i diabetes mellitus
Y. M. Kolesnik, A.V. Trailin, M.A. Orlovsky
Запоріз.мед.ун-т МОЗ України
Abstract
The aim of this work was to study manifestations of apoptosis
in pancreas, brain and myocardium of rats at the type I
diabetes mellitus. To reveal the apoptotic changes in cells,
specific damages of DNA were identified by the TUNEL-method. Specific for apoptosis changes in chromatin and cell
membranes were found after counterstaining of a specimen
by methyl green. In addition, the apoptotic and non-apoptotic cells were differentiated automatically with the help of
image analyzing computer system by an index of optical density of cellular nucleus. This method allowed to find out for
sure that apoptotic cells were available in all the studied tissues of healthy animals. However the possibility of their
revealing by the TUNEL-method depends on the way of
tissue fixation. Development of the type I diabetes mellitus
was accompanied with an increase in the number of such
cells, which gives evidence for an important role of apoptosis
in diabetes pathogenesis.
References
- Балаболкин М. Диабетология. – М.: Мир, 2000. – 672 с.
- Дедов И.И. Сахарный диабет в Российской Федерации: проблемы и пути решения // Сахарный диабет. – 1998. – № 1. – С.7 – 19.
- Колесник Ю.М., Василенко Г.В., Абрамов А.В. Возможные механизмы гипоталамической регуляции эндокринной функции поджелудочной железы у мышей диабетической линии С57BL/KsJ // Архив патологии. – 1994. – 56, № 4. – С. 56 – 60.
- Симоненко В.Б., Бойцов С.А., Глухов А.А. Апоптоз и патология миокарда // Клин. медицина. – 2000. – № 8. – С. 12 – 16.
- Фильченков А.А., Стойка Р.С. Апоптоз и рак. – К.: Морион, 1999. – 1984 с.
- Arends M.J., Morris R.G., Wyllie A.H. Apoptosis. The role of the endonuclease // Amer. J. Pathol1. – 1990. – 136. – P. 593 – 608.
- Aughsteen A.A. An ultrastructural study on the effect of streptozotocin on the islets of Langerhans in mice // J. Electron/ Microsc. (Tokyo). – 2000. – 49, №.5. – P. 681 – 690.
- Banasiaka K.J., Xiab Y., Haddad G.G. Mechanisms underlying hypoxia-induced neuronal apoptosis // Progress in Neurobiology. – 2000. – 62. – P. 215 – 249.
- 9. Carson D., Ribeiro J. M. Apoptosis and disease // Lancet. – 1993. – 341. – P. 1251 – 1254.
- 10. Chen S., Evans T., Mukherjee K. et. al. Diabetes-induced myocardial structural changes: role of endothelin-1 and its receptors // J. Mol. Cell. Cardiol. – 2000. – 32, № 9. – P. 1621 – 1629.
- Geng Y.J. Regulation of programmed cell death or apoptosis in atherosclerosis // Heart Vessels. – 1997. – 12. – P. 76 – 80.
- Gravrieli Y., Sherman Y., Ben-Sasson S.A. Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation // J. Cell. Biol. – 1992. – 119. – P. 493 – 501.
- Kajstura J., Fiordaliso F., Andreoli A.M. et. al. IGF-1 overexpression inhibits the development of diabetic cardiomyopathy and angiotensin II-mediated oxidative stress // Diabetes. – 2001. – 50, №.6. – P. 1414 – 1424.
- Kerr J. F., Wyllie A. H., Currie A. R. Apoptosis: a basic biological phenomenon with wide-ranging implications in tissue kinetics // Brit. J. Cancer. – 1972. – 26. – P. 239 – 257.
- Li C., Li P.A., He Q.P. et. al. Effects of streptozotocininduced hyperglycemia on brain damage following transient ischemia // Neurobiol. Dis. – 1998. – 5, № 2. – P. 117 – 128.
- Logsdon M.D., Meyn R.E. Jr, Besa P.C. et al. Apoptosis and the Bcl-2 gene family – patterns of expression and prognostic value in stage I and II follicular center lymphoma // Int. J.Radiat. Oncol. Biol.Phys. – 1999. – 44. – P. 19 – 29.
- Mauricio D., Mandrup-Poulsen T. Apoptosis and the pathogenesis of IDDM. A question of life and death // Diabetes. – 1998. – 47. – P. 1573 – 1543.
- Moriwaki M., Itoh N., Miyagawa J. et. al. Fas and Fas ligand expression in inflamed islets in pancreas sections of patients with recentonset Type I diabetes mellitus // Diabetologia. – 1999. – 42, № 11. – P. 1332 – 1340.
- 19. Piotrowski P., Gajkowska B., Olszewska H., Smialek M. Electron microscopy studies on experimental diabetes and cerebral ischemia in the rat brain // Folia Neuropathol. – 1999. – 37, № 4. – P. 256 – 263.
- 20. Russell J.W., Feldman E.L. Insulin-like growth factor-I prevents apoptosis in sympathetic neurons exposed to high glucose // Horm. Metab. Res. – 1999. – 31, № 2 – 3. – P. 90 – 96.
- Sahu A., Sninsky C.A., Kalra P.S., Kalra S.P. Neuropeptide Y concentrations in microdissected hypothalamic regions and in vitro release from the medial basal hypothalamus-preoptic area of streptozotocin-diabetic rats with and without insulin substitution therapy // Endocrinology. – 1990. – 126. – P. 192 – 198.
- Saviani E.E., Orsi C.H., Oliveira J.F. et al. Participation of the mitochondrial permeability transition pore in nitric oxide-induced plant cell death // FEBS Lett. – 2002. – 510, № 3. – P. 136 – 140.
- Smaili S.S., Hsu Y.T., Youle R.J., Russell J.T. Mitochondria in Ca2+ signaling and apoptosis // J. Bioenerg. Biomembr. – 2000. – 32, № 1. – P. 35 – 46.
- Takeuchi M., Bucala R., Suzuki T. et. al. Neurotoxicity of advanced glycation end-products for cultured cortical Neurons // J. Neuropathol. Exp. Neurol. – 2000. – 59, №12. – P. 1094 – 1105.
- Tamura M., Kimura H., Koji T. et. al. Role of Apoptosis of Thyrocytes in a Rat Model of Goiter. A Possible Involvement of Fas System // Endocrinology. – 1998. – 139, № 8. – P. 3646 – 3653.
- Trump, B.F., Berezesky, I.K., Cowley, R.A., 1982. The cellular and subcellular characteristics of acute and chronic injury with emphasis on the role of calcium. – In: Pathophysiology of shock, anoxia and ischemia. Baltimore. – Williams and Wilkins, 1982. –Р. 6 – 46.
- Walker N.I., Harmon B.V., Gobe G.C., Kerr J.F.R. Patterns of cell death //Methods Achiev. Exp. Pathol. – 1988. – 13. – P. 18 – 54.
- Wang R.N., Rosenberg L. Maintance of beta-cell function and survival following islet isolation requires re-establishment of the islet-matrix relationship // J.Endocrinol. – 1999. – 163. – P. 181 – 190.
- 29. Wolozin Z. Neuronal loss in aging and disease. – In: Cellular Aging and Cell Death. New York: WileyLiss, 1996. – Р. 289 – 300.
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