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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2003; 49(5): 82-88


Study of apoptosis manifestations intype i diabetes mellitus

Y. M. Kolesnik, A.V. Trailin, M.A. Orlovsky

    Запоріз.мед.ун-т МОЗ України


Abstract

The aim of this work was to study manifestations of apoptosis in pancreas, brain and myocardium of rats at the type I diabetes mellitus. To reveal the apoptotic changes in cells, specific damages of DNA were identified by the TUNEL-method. Specific for apoptosis changes in chromatin and cell membranes were found after counterstaining of a specimen by methyl green. In addition, the apoptotic and non-apoptotic cells were differentiated automatically with the help of image analyzing computer system by an index of optical density of cellular nucleus. This method allowed to find out for sure that apoptotic cells were available in all the studied tissues of healthy animals. However the possibility of their revealing by the TUNEL-method depends on the way of tissue fixation. Development of the type I diabetes mellitus was accompanied with an increase in the number of such cells, which gives evidence for an important role of apoptosis in diabetes pathogenesis.

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