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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2003; 49(4): 7-13

Factor released under heart reperfusion may be themarker of opening of the mitochondrial permeability transition pore

Sagach V.F., Shymanskaya T.V., Nadtochiy S.M

    A.A. Bogomoletz Institute of Physiology National Academy ofSciences of Ukraine



Abstract

In experiments on isolated hearts of guinea-pigs, on a model of ishaemia-reperfusion cardiac reperfusion has been shown to result in a constriction of coronary vessels, arrhythmia, an inhibition of the contractile activity of the myocardium, and an increase in an oxygen cost of the myocardial work . Apart from that, a stable agent was detected in a reperfusion solution by spectrophotometry on the wave length of 250 nM. Similar deterioration of the heart function and the availability of the stable agent in the solution were observed under influence of the known activators of mitochondrial permeability transition pore-phenilarsine oxide and antimycin A. In anaesthetized animals a release of the stable factor into the blood was induced by either ishaemia - reperfusion or those activators. Application of the known inhibitors of the mitochondrial permeability transition pore cyclosporin A or trolox (water-soluble vitamin E) decreased remarkably both the cardiodynamic deterioration and a release of the stable factor under heart reperfusion. Mitochondrial origin of the factor was confirmed in experiments on isolated mitochondria. Thus, the detected factor has been determined to be released from mitochondria at the opening of mitochondrial permeability transition pore and is thought to be the marker of its opening in experiments in vitro and in vivo.

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