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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2000; 46(3): 84-91

The influenceof Thyroxine on Activity of Energy Metabolism and Antioxidative Systemsof Piglet Neutrophils

Babych N. O., Antonyak H. L., Tymochko M. F., Snitynsky V. V

    Lviv Medical University, Lviv, Ukraine;Institute of Agriculture and Biology of Animals, Lviv, Ukraine



Abstract

The influence of thyroxine on activity of enzymes of energy metabolism (hexokinase, phosphofructokinase, pyruvate kinase, laktate dehydrogenase, glucose-6-phosphate dehydrogenase, NADP-isocytrate dehydrogenase, cytochrome-c oxidase ) and antioxidative system (glutathione peroxidase, glutathione reductase, superoxide dismutase) of neonatal piglet neutrophils was investigated. It has been found, that after durable injections of hormone (4 mg/kg body weight ) the increase of glycolytic enzymes activities as well as aerobic energy pathway catalyzators took place. Simultaneously the augmentation of superoxide dismutase reaction occured after the thyroxine treatment. Such effect might represent an important link in compensatory mechanism, which prevents the distructive action of reactive oxygen species.

Full text: (PDF, in Ukrainian)

References

  1. Антоняк Г.Л. Визначення активності глутатіонредуктази. — В кн.: Методикидосліджень з фізіології і біохімії сільськогосподарських тварин. — Львів, 1998.—С. 91.
  2. Антоняк Г.Л., Бучко О.М. Визначення активності глутатіонпероксидази. — Тамже. — С. 90-91.
  3. Антоняк Г.Л., Бучко О.М. Визначення активності супероксиддисмутази / Тамже. — С. 89-90.
  4. Астауров Б.Л. Методы биологии развития. — М., 1974. — С.346-433.
  5. Дубинина Е.Е., Сальникова Л.А., Ефимова Л.Ф. Активность и изоферментныйспектр супероксиддисмутазы эритроцитов и плазмы крови человека // Лаб.дело. — 1983. — № 10. — С. 30-33.
  6. Зенков Н.К., Меньщикова Е.Б. Активированные кислородные метаболиты в био-логических системах // Успехи совр. биологии. — 1993. — 113, № 3. — С. 286-296.
  7. Моин В.М. Простой и специфический метод определения активности глутатион-пероксидазы в эритроцитах // Лаб. дело. — 1986. — № 12. — С. 724-727.
  8. Рачев Р.Р., Ещенко Н.Д. Тиреоидные гормоны и субклеточные структуры. — М.:Медицина, 1975. — 294 с.
  9. 9. Снітинський В.В., Антоняк Г.Л. Біохімічна роль селену // Укр. біохім. журн.—1994. — 66, № 5. — С. 3-16.
  10. 10. Bцyum A.A. A one- stage procedure for isolation of granulocytes and lymphocytesfrom human blood// Scand. J. Clin. Lab. Invest. — 1968. — 21. — P.21.
  11. Clark R.A. The human neutrophil respiratory burst oxidase// J. Infect. Dis. —1990. — 161. — P. 1140-1147.
  12. Cooperstein S.J., Lazarow A. A microspectrophotometric method for the determina-tion of cytochrome- c oxidase // J. Biol. Chem. — 1951. — 189. — P. 665-670.
  13. Cristensen R.D. Granulocytopoiesis in the fetus and neonate // Transfus. Med.Rev. — 1990. — 4, №1. — P. 8-13.
  14. Da Silva F.M., Massart-Leen A.M., Burvenich C. Development and maturation ofneutrophils // Vet. Q. — 1994. — 16, № 4. — P. 220-225.
  15. Demple B. Regulation of bacterial oxidase stress genes // Annu. Rev. Genet. —1991. — 25. — P. 315-337.
  16. Everse J., Kaplan N.O. Mechanisms of action and biological function of variousdehydrogenase isozymes — In: Isozymes. — New York, Acad. Press, 1975. — PartII. — P. 29.
  17. Foetal and Neonatal Immunology / Ed. by J. B. Solomon. — North-Holland Publ.Company, Amsterdam- London. — 1971. — 381 p.
  18. Immunology of the Neonate / Eds. G.R.Burgio, L.A.Hanson, A.G.Ugazio. —Springer-Verlag-Berlin-Heidelberg-New York-London-Paris-Tokyo, 1987. — 188 p.
  19. 19. Mьller M.J. Thyroid hormones and energy fat balance // Eur. J. of Endocrinol.—1997. — 136. — P. 267-268.
  20. 20. Mьller S., Seitz H.J. Cloning of cDNA for the FAD-linked glycerol-3-phosphatedehydrogenase from rat liver and its regulation by thyroid hormones // Proc.Natl. Acad. Sci. USA. — 1994. — 91. — P. 10581-10585.
  21. Simon O., Bergner H. Effects of thyroid hormones on tissue protein turnover //Acta Biol. Med. Germ. — 1981. — 40, №10/11. — P. 1235-1238.
  22. Strauss R.G., Mauer A.M. Formed elements of human blood // In: PerinatalPhysiology. — Ed. U. Stave. — Plenum Medical Book Comp., New York-London,1978. — P. 199-213.
  23. Weismann I.L. Developmental switches in the immune system // Cell. — 1994.—76, № 1. — P. 207-218.
  24. Wiesner R.J., Kurowski T.T., Lak R. Regulation by thyroid hormone of nuclearand mitochondrial genes encoding subunits of cytochrome-c oxidase in the rat liverand sceletal muscle // Mol. Endocrinol. — 1992. — 6. — P. 1458-1467.
  25. Yu B.P. Cellular defences against damage from reactive oxygen species // Physiol.Rev. — 1994. — 74, №1. — P. 139-162.
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