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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2003; 49(1): 61-66

Peculiarities of testosterone influence on activity of prostate cells. proteins synthesisunder conditions of hypo- and hyperprolactinemia

T. Bondarenro

    Ін-т проблем ендокрин. патологіїім. В.Я. Данилевського АМН України, Харків



Abstract

The influence of testosterone on the concentration and synthesis of nuclear and cytoplasmic prostate proteins were studed in rats with hypo- and hyperprolactinemia. Hyperprolactinemia was induced by “Lactin”, and the hypoprolactinemia was modelled by ”Parlodel”. The rats were injected with 14С-protein hydrolysate i.p. for the investigation of the proteins biosynthesis and concentration of prostate cells. The androgen inhibited catabolism of nonhistone proteins and the proteins of nuclear juice in intact rats organism. Testosterone increased the synthesis of all proteins in rats with prolactine deficiency, but this enlarge was not connected with an elevation of their concentration. Thus, we suggest that in this case the proteins disintegration may be increased. Under hyperprolactinemia the concentration of all proteins, besides nonhistone proteins, was declined that reflected the enhancement their catabolism.

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References

  1. Закс Л. Статистическое оценивание. – М.: Статис тика, 1976. – 598 с.
  2. Збарский И.Б. Организация клеточного ядра. – М.: Медицина, 1988. – 336 с.
  3. Збарский И.Б., Георгиев Г.П. Новые данные по фракционированию клеточных ядер печени крысы и химическому составу ядерных структур // Био- химия. – 1959. – 24, № 2. – С. 192.
  4. Прокофьева-Бельговская А.А. Значение негисто- новых белков в преобразованиях и генетическом функционировании хромосом // Молекуляр. био- логия. – 1982. – 16, № 4. – С. 771 – 781.
  5. Романов Г.А., Соколова Н.А., Розен В.Б. и др. Взаи- модействие дексаметазон-рецепторных комплексов с ядрами печени крысы и с ДНК // Биохимия. – 1976. – 41, № 12. – С. 2140 – 2144.
  6. Уманский С.Р., Токарская В.И., Зотова В.А. и др. Выделение и гетерогенность негистоновых белков хроматина печени крысы // Молекуляр. биология. – 1971. – 5, № 2. – С. 270 – 279.
  7. An W., van Holde K., Zlatanova J. The non-histone chromatin protein HMG1 protects linker DNA on the side opposite to that protected by linker histones // J. Biol. Chem. – 1998. – 273, № 41. – Р. 26289 – 291.
  8. Baranao J., Legnani B., Chiauzzi V. et al. Effects of prolac- tin on androgen met.abolism in androgen target tissues of immature rats // Endocrinology. – 1981. – 109, № 6. – P. 2188 – 2195.
  9. 9. Hiremath S.T., Maciewicz R.A., Wang T.Y. The loosely bound non-histone chromosomal proteins of rat prostate in androgen action // Biochim. and Biophys. Acta. – 1981. – 653, № 1. – P . 130 – 138.
  10. 10. Johns E.W., Butler J. Futher fractionation of histones from calf thymus // Biochem. J. – 1962. – 82, № 1. – P. 15 – 18.
  11. Kabler R.L., Srinivasan A., Taylor L.J. et al. Andro- gen regulation of ribosomal RNA synthesis in LN- CaP cells and rat prostate // J. Steroid Biochem. Mol. Biol. – 1996. – 59, № 5–6. – P. 431-439.
  12. McPherson S. J., Wang H., Jones M. E. et al. Elevated Androgens and Prolactin in Aromatase-Deficient Mice Cause Enlargement, But Not Malignancy, of the Prostate Gland // Endocrinology. – 2001. – 142. – P. 2458 – 2467.
  13. Okuda Y., Fujisawa M., Matsumoto O., Kamidono S. Tes- tosterone dependent regulation of the enzymes involved in DNA synthesis in the rat ventral prostate // J. Urol. – 1991. – 145, № 1. – P. 188 – 191.
  14. Orensfein J.M., March W.H. Incorporation in vivo of me- thionine and ethionin into the methylation and ethylation of rat liver nuclear proteins // Biochem. J. – 1968. – 109. – P. 697 – 699.
  15. Perez-Villamil B., Bordiu E., Puente-Cueva M. Involve- ment of physiological prolactin levels in growth and pro- lactin receptor content of prostate glands and testes in development male rats // J. Endocrinol. – 1992. – 132. – P. 449 – 459.
  16. Rui H., Haug E., Mevag B., Thomassen Y., Purvis K. Short- term effecs of prolactin on prostatic function in rats with lisuride-induced hyperprolactinaemia // J. Re- prod. and Fertil. – 1985. – 75, № 2. – P. 421 – 432.
  17. Sluyser M. Effect of testosterone on the binding of pros- tate histone to DNA in vitro // Biochem. and biophys. Res. Commun. – 1966. – 22, № 3. – P. 336 – 339.
  18. Strick, R. Laemmli,U.K. SARs are cis DNA elements of chromosome dynamic synthesis of a SAR repressor pro- tein // Cell. – 1995. – 83. – P. 1137 – 1148.
  19. 19. Van Coppenolle F., Slomianny C., Carpentier F. et al. Ef- fects of hyperprolactinemia on rat prostate growth: evi- dence of androgeno-dependence // Amer. J. Physiol. Endo- crinol. Metab. – 2001. – 280. – P. E120 – E129.
  20. 20. Wennbo H., Kindblom J., Isaksson O.G., Tornell J. Trans- genic mice overexpressing the prolactin gene develop dramatic enlargement of the prostate gland // Endo- crinology.– 1997. – 138, №10. – P. 4410 – 4415.
  21. Zhang Cai-Qiao, Zhuang Lin-Zhi, Yang Chuan-Ren. Влия- ние ПРЛ на образование кислой фосфотазы и ди- гидротестостерона в эпителиальных клетках ПЖ половозрелых крыс// Dongwu xuebao=Acta zool. sin. – 1994. – 40, №4. – P. 264 – 267.
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