Many types of cells release ATP under the action of specific physiological signals. It is known that the ATP and other adenyl nucleotides is involved in the regulation of bile secretion too. The aim of our experiments was to determinate the influence of noradrenaline, fMLP and verapamile on the biliary secretion of adenyl nucleotides in bile-fistula rats. The biliary secretion of adenyl nucleotides was decreased under the influence of noradrenaline, fMLP (in doses 0,1 mkg/(100 g*30 min) and 10,0 mkg/(100 g*30 min)), verapamile and successive intraportal infusion of verapamile and noradrenaline (in 90 mM/l CaCl2 solution). It seems to be possible that noradrenaline, fMLP and verapamile regulate bile secretion on the level of energetic exchange.