The naturally occurring endotoxin lipopolysaccharide (LPS) can cause systemic endotoxemia and impair animal reproductive capacity. LPS induces oxidative stress and alters protein, receptor expression, and metabolic pathways in the uterus, impairing its function. The aim of the work was to investigate the effect of exogenous glutathione on oxytocin-induced contractile activity and basal tone of isolated uterine myometrium preparations from rats with LPS-induced experimental endotoxemia. The experiments were performed on isolated longitudinal strips of the uterine horn of mature estrogenic female Wistar rats perfused at 37°C with oxygenated Krebs solution. All testing was performed in isometric mode at an initial set tension of 3 mN. The animals were divided into three groups: control, rats injected with LPS, and rats injected with LPS and glutathione. Experimental endotoxemia was created by intraperitoneal administration of lipopolysaccharide at a dose of 3 mg/kg the day before the experiment. Glutathione was administered intraperitoneally at 52 mg/kg twice, one day and one hour before the experiment. Oxytocin was added to the perfusion solution at a 0.1-100 nmol/L concentration. Oxytocin dose-dependently increased the amplitude of contractions of isolated preparations of rat uterine myometrium with a maximum effect of 26.5%. At a concentration of 100 nmol/L, a significant increase in basal tone and contraction frequency of myometrial strips of 6.5% and 66.8%, respectively, was observed. In LPS-in- duced experimental endotoxemia, the contractile activity of myometrial preparations in response to oxytocin was significantly increased. At the same concentration of oxytocin, the amplitude of contractions and basal tone compared to the control group increased by 3.1 and 2.3 times, respectively, and the frequency of contractions by 71% compared to baseline. Glutathione administration to animals significantly reduced the effect of LPS on oxytocin-induced changes in the contractile activity of the uterine myometrium. The contraction amplitude, basal tone, and contraction frequency in these rats were close to the values in control rats. Hence, in LPS-induced experimental endotoxemia, the amplitude of oxytocin-induced contraction and the basal tone of uterine preparations significantly increased, which was prevented by glutathione administration to the animals. This suggests the possible use of the antioxidant glutathione in endotoxemia to prevent impaired reproductive function in animals.