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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2020; 66(1): 60-67

TOXICOLOGICAL STUDIES STRUCTURALMETABOLITIC COMPLEXES OF PROBIOTIC STRAINS OF LACTOBACILLUS RHAMNOSUS GG AND SACCHAROMYCES BOULARDII IN TESTS IN VIVO

O.Y. Isayenko

    SE “I. Mechnikov Institute of Microbiology and Immunology of NAMS of Ukraine”, Ukraine
DOI: https://doi.org/10.15407/fz66.01.060


Abstract

The absence of toxic substances and harmful impurities in structural-metabolitic complexes of L. rhamnosus and S. boulardii, obtained by the author’s method without using nutrient media, was proved. Structural components were obtained by low-frequency ultrasound treatment of Lactobacillus rhamnosus GG or Saccharomyces boulardii cells and metabolites by cultivating lactobacteria and saccharomycetes in disintegrators of probiotic microorganisms. When administered in different doses (0.5, 1.0, 2.0 ml), structural-metabolitic complexes of L. rhamnosus and S. boulardii did not reveal a dose dependence. Due to skin and conjunctival tests in guinea pigs, the absence of allergenic properties of the substances was proved. Obtaining original metabolitic compounds by cultivating L. rhamnosus and S. boulardii with the structural components of bacteria or fungi eliminates the possible negative influence of the nutrient medium on the final product and opens the prospects for creating a new class of structural and metabolitic substances and their combinations. This, in turn, will expand the range of search for alternative, additional or improve existing treatment prophylactic probiotic preparations with their subsequent application in diseases of different genesis.

Keywords: metabolites; structural components; specific harmlessness; acute and chronic toxicity; Lactobacillus rhamnosus GG; Saccharomyces boulardii

Full text: (PDF, in Ukrainian)

References

  1. On the protection of animals used for scientific purposes. European Parliament and of the Council. Directive 2010. 63. EU. 22 Sept 2010.
    2.  
  2. Kojima H. JaCVAM: an organization supporting the validation and peer review of new alternatives to animal testing. Proc. 6th World Congress on Alternatives & Animal Use in the Life Sciences. Tokyo, Japan. 2007;AATEX 14:483-5.
    3.  
  3. Suppression of the test for abnormal toxicity from the European Pharmac. European Pharmacopoeia animal testing. Strasbourg, France. 08 Dec 2017.
    4.  
  4. State Pharmacopoeia of Ukraine. State enterprise "Ukrainian scientific pharmacopoeial center for quality of medicines", Kh. State Enterprise "Ukrainian scientific pharmacopoeial center for quality of medicines". 2015; 2(1):527. [Ukrainian].
    5.  
  5. State Pharmacopoeia of the Russian Federation. Publishing House Scientific center for expertise of medical devices. 2015;XIII:1469. [Russian].
    6.  
  6. Kulakova YuV. Development of a multicomponent metabolite probiotic [dissertation]. Moscow research institute of epidemiology and microbiology G.N. Gabrichevsky. 2013. [Russian].
    7.  
  7. Safonova GM, Neschislayev VA, Chistokhina LP. Safety assessment of a new probiotic containing lactobacilli metabolites. Sib M J. 2004;19(2):38-40. [Russian].
    8.  
  8. Startseva NV. Immunobiological characteristics of the drug "Biostim-T" based on natural nucleic acids [thesis]. Perm: Institute of Ecology and Genetics of microorganisms of the Ural Branch RAS; 2004. [Russian].
    9.  
  9. Vasilevich SF. Probiotic-enterosorbent Sorbolin production technology and application efficiency in cattle breeding. [abstract of dissertation]. Moscow: Moscow state academy of veterinary medicine and biotechnology MVA named after K.I. Scriabin; 2018. [Russian].
    10.  
  10. Isayenko OYu, Knysh OV, Falko OV, Prokopyuk VYu, Prokopyuk OS. Cytotoxicity structural-metabolitic complexes of Lactobacillus rhamnosus GG and Saccharomyces boulardii. Fiziol Zh. 2019;65(5):40-8. [Ukrainian]. CrossRef  
  11. Isayenko OYu, Knysh OV, Babych YeM, Kivva FV, Horbach TV, Balak OK. inventor; DU«IMI NAMN», assignee. Method of producing metabolites of probiotic bacterial strains. Ukrainian patent UA 123122. 2018 Feb.  [Ukrainian].
    12.  
  12. Isayenko OYu, Knysh OV, Babych YeM, Vashchenko V, Zachepylo SV, Polyanska VP, Kovalenko OI, Balak OK. inventor; DU«IMI NAMN», assignee. A method of obtaining a combination of metabolites of probiotic strains of fungi and bacteria. Ukrainian patent. UA 126603. 2018 Jun. 25. [Ukrainian].
    13.  
  13. Eliseeva TI, Balabolkin II. Allergic reactions to medicines: current concepts (overview). Mod Technolog Med. 2016;8(1):159-73. [Russian]. CrossRef

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