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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2020; 66(1): 60-67


TOXICOLOGICAL STUDIES STRUCTURALMETABOLITIC COMPLEXES OF PROBIOTIC STRAINS OF LACTOBACILLUS RHAMNOSUS GG AND SACCHAROMYCES BOULARDII IN TESTS IN VIVO

O.Y. Isayenko

    SE “I. Mechnikov Institute of Microbiology and Immunology of NAMS of Ukraine”, Ukraine
DOI: https://doi.org/10.15407/fz66.01.060

Abstract

The absence of toxic substances and harmful impurities in structural-metabolitic complexes of L. rhamnosus and S. boulardii, obtained by the author’s method without using nutrient media, was proved. Structural components were obtained by low-frequency ultrasound treatment of Lactobacillus rhamnosus GG or Saccharomyces boulardii cells and metabolites by cultivating lactobacteria and saccharomycetes in disintegrators of probiotic microorganisms. When administered in different doses (0.5, 1.0, 2.0 ml), structural-metabolitic complexes of L. rhamnosus and S. boulardii did not reveal a dose dependence. Due to skin and conjunctival tests in guinea pigs, the absence of allergenic properties of the substances was proved. Obtaining original metabolitic compounds by cultivating L. rhamnosus and S. boulardii with the structural components of bacteria or fungi eliminates the possible negative influence of the nutrient medium on the final product and opens the prospects for creating a new class of structural and metabolitic substances and their combinations. This, in turn, will expand the range of search for alternative, additional or improve existing treatment prophylactic probiotic preparations with their subsequent application in diseases of different genesis.

Keywords: metabolites; structural components; specific harmlessness; acute and chronic toxicity; Lactobacillus rhamnosus GG; Saccharomyces boulardii

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