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TOXICOLOGICAL STUDIES STRUCTURALMETABOLITIC COMPLEXES OF PROBIOTIC STRAINS OF LACTOBACILLUS RHAMNOSUS GG AND SACCHAROMYCES BOULARDII IN TESTS IN VIVO
O.Y. Isayenko
SE “I. Mechnikov Institute of Microbiology and Immunology of NAMS of Ukraine”, Ukraine
DOI: https://doi.org/10.15407/fz66.01.060
Abstract
The absence of toxic substances and harmful impurities in
structural-metabolitic complexes of L. rhamnosus and S. boulardii,
obtained by the author’s method without using nutrient
media, was proved. Structural components were obtained by
low-frequency ultrasound treatment of Lactobacillus rhamnosus
GG or Saccharomyces boulardii cells and metabolites by
cultivating lactobacteria and saccharomycetes in disintegrators
of probiotic microorganisms. When administered in different
doses (0.5, 1.0, 2.0 ml), structural-metabolitic complexes of
L. rhamnosus and S. boulardii did not reveal a dose dependence.
Due to skin and conjunctival tests in guinea pigs, the
absence of allergenic properties of the substances was proved.
Obtaining original metabolitic compounds by cultivating L.
rhamnosus and S. boulardii with the structural components
of bacteria or fungi eliminates the possible negative influence
of the nutrient medium on the final product and opens the
prospects for creating a new class of structural and metabolitic
substances and their combinations. This, in turn, will expand
the range of search for alternative, additional or improve
existing treatment prophylactic probiotic preparations with
their subsequent application in diseases of different genesis.
Keywords:
metabolites; structural components; specific harmlessness; acute and chronic toxicity; Lactobacillus rhamnosus GG; Saccharomyces boulardii
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