Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2020; 66(1): 44-51


Yu.V. Fedorenko, M.R. Gzhegotsky

    Danylo Halytsky Lviv National Medical University, Ukraine


The dynamics of individual biochemical parameters in blood serum of 72 white rats with active and passive type of behavior under the combined effects of immobilizing stress and cadmium during 30 days was investigated. The type of behavior was determined using the open field test. The combined effect of factors was simulated by the daily injection into the stomach of an aqueous solution of cadmium sulfate followed by keeping animals in separate plastic boxes for 6 h. Multidirectional changes in the concentration of calcium were revealed – an increase up to 10 days of experiments, subsequently – a decrease of the concentration of calcium in both groups of the animals, at the end of experiments – in the passive ones by 43.8%, in the active ones – by 28.1%; an increase of glucose concentration in active animals in the range of 10.0-29.7%, in the passive ones – in the range of 13.0-38.0%, hypercholesterolemia with a tendency to decrease the concentration of total cholesterol at the end of the experiments in the active animals and an increase in the passive ones compared with the previous terms of observation. Alkaline phosphatase activity was increased at the beginning of the experiment, there was a decrease of enzyme activity on the 10th day in passive animals and on the 30th days in both groups, especially in the passive group – by 45.5%. The activity of aminotransferases increased from the 5th day and at the end of the experiment, the activity of alanineaminotransferase in passive animals was higher by 48.7%, in the active ones – by 31.9% compared to the control. Metabolic changes occur as a result of a combination of nonspecific effects of immobilization stress and direct toxic effects of cadmium on an organism. Intensity and orientation of the studied indicators depend on the individual and typological features of the CNS. More pronounced changes were observed in passive animals.

Keywords: immobilization stress; cadmium; combined effect; active and passive animals; calcium; glucose; alkaline phosphatase; cholesterol; transferases.


  1. Grigoryan AG. Changing the content of total lipids in the blood and body weight of the animals at a hypokinesia. Biol Zh Armenii. 2012;3(64):66-9. [Russian].
  3. Stepanchuk VV. Immobilization stress and chronorythms of humoral immunity in albino rats. Health and Education in the 21st century. 2013;1-4(15):227-9. [Russian].
  5. Havreliuk SV, Chykina IV. Effect of chronic immobilisation stress on the development of endothelial dysfunction in rats. Fiziol Zh. 2017;63(2):56-64. [Ukrainian]. CrossRef  
  6. Timchenko OI, Omelchenko EM, Belitskaya EN, Glavatskaya VI, Golovkova TA, Vigovskaya TA, Shumilo AM, Tsyiguleva OM. Heavy metals (lead, cadmium, mercury) as environmental pollutants in Ukraine. Kyiv; 2008:77. [Russian].
  8. Fedorenko VI. The substantiation of the maximum daily permissible doses of the lead and cadmium in everyday diet. Med Perspect. 2019;24(1):73-80. [Ukrainian]. CrossRef   International program on chemical safety environmental health criteria. 134. Cadmium: World Health Organization Geneva, 1992. Available from: http:.www.inchem. org. documents. ehc. ehc. ehc134.htm   Robin A Bernhoft. Cadmium toxicity and treatment. Sci World J. 2013;2013(ID 394652):[7 pages]. Available from: 10.1155. 2013. 394652  
  9. Ismaylova KhYu, Agaev TM, Semenova TP. Individual pecularities of the behavior: (monoaminergic mechanisms). Baku: Nurlan; 2007:228. [Russian].
  11. Bahshalieva AJa. Features of development of depressive state in rats with different individual-typological status. Neurophysiology. 2010;42(2):153-61. [Russian]. CrossRef  
  12. Karpovskyi VV, Trokoz VO, Karpovskyi VI, Danchuk OV, Postoi RV. Cortical regulation of exchange of lipids in pigs. Kyiv: DDP «Ekspo-Druk»; 2017. [Ukrainian].
  14. Karpovskiy VI, Zhurenko OV, Trokoz VO, Postoy RV, Sysyuk YuO, Kravchenko-Dovha YuV, Landarenko LS. Cortico-vegetative relations in the regulation of physiological functions in cows. Scientific Messenger of LNUVMBT named after S.Z. Gzhytskyj. 2016;18(1Pt 2):64-9. [Ukrainian].
  16. Fedorenko YuV. Biochemical parameters and adaptation of white rats with active and passive type of behavior under immobilization stress. Med Perspect. 2016;21(2):13-9. [Ukrainian]. CrossRef   Khopta NS, Ersteniuk AM. Metabolic changes in the bone tissue of animals under experimental cadmiosis. Scientific J. "Science Rise: Biological Science". 2018;5(14):31-5. [Ukrainian]. Available from: DOI: 10.15587. 2519- 8025.2018.147090 CrossRef  
  17. Wu Si, Kot Yu, Kot K, Morse R, Doran O, Hamdallah Amjad, Persky E. Total metabolism and oxidative stress parameters in rats at long-term exposure to low concentrations of Cd2+. J V.N. Karazin Kharkiv Nat Univ. Ser: Biology. 2017;(29):182-3. [Russian].
  19. Panin LE. Biochemical mechanisms of stress. Novosibirsk: Nauka; 1983:233. [Russian].
  21. Melnikova NM, Shepelova IA, Derkach EA, Motornyuk AV. Biochemical characteristic of blood of rabbits, poisoned with cadmium sulfate. Scientific Messenger of LNUVMBT named after S.Z. Gzhytskyj. 2009;1(Pt 2):227-30. [Ukrainian].
  23. Kalinin IV. Efficiency of aluminosilicates for reducing heavy metals in rat tissues. Fiziol Zh. 2017;63(5):41-8. [Ukrainian]. CrossRef  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2020.