|
|
|
|
|
|
THE INFLUENCE OF THE COMBINED EFFECT OF IMMOBILIZATION STRESS AND CADMIUM ON THE DYNAMICS OF BIOCHEMICAL PARAMETERS IN RATS WITH ACTIVE AND PASSIVE TYPE OF BEHAVIOR
Yu.V. Fedorenko, M.R. Gzhegotsky
Danylo Halytsky Lviv National Medical University, Ukraine
DOI: https://doi.org/10.15407/fz66.01.044
Abstract
The dynamics of individual biochemical parameters in blood
serum of 72 white rats with active and passive type of behavior
under the combined effects of immobilizing stress and cadmium
during 30 days was investigated. The type of behavior
was determined using the open field test. The combined effect
of factors was simulated by the daily injection into the stomach
of an aqueous solution of cadmium sulfate followed by keeping
animals in separate plastic boxes for 6 h. Multidirectional
changes in the concentration of calcium were revealed – an
increase up to 10 days of experiments, subsequently – a decrease
of the concentration of calcium in both groups of the
animals, at the end of experiments – in the passive ones by
43.8%, in the active ones – by 28.1%; an increase of glucose
concentration in active animals in the range of 10.0-29.7%,
in the passive ones – in the range of 13.0-38.0%, hypercholesterolemia
with a tendency to decrease the concentration of
total cholesterol at the end of the experiments in the active
animals and an increase in the passive ones compared with the
previous terms of observation. Alkaline phosphatase activity
was increased at the beginning of the experiment, there was a
decrease of enzyme activity on the 10th day in passive animals
and on the 30th days in both groups, especially in the passive
group – by 45.5%. The activity of aminotransferases increased
from the 5th day and at the end of the experiment, the activity
of alanineaminotransferase in passive animals was higher by
48.7%, in the active ones – by 31.9% compared to the control.
Metabolic changes occur as a result of a combination of nonspecific
effects of immobilization stress and direct toxic effects
of cadmium on an organism. Intensity and orientation of the
studied indicators depend on the individual and typological
features of the CNS. More pronounced changes were observed
in passive animals.
Keywords:
immobilization stress; cadmium; combined effect; active and passive animals; calcium; glucose; alkaline phosphatase; cholesterol; transferases.
References
- Grigoryan AG. Changing the content of total lipids in the blood and body weight of the animals at a hypokinesia. Biol Zh Armenii. 2012;3(64):66-9. [Russian].
- Stepanchuk VV. Immobilization stress and chronorythms of humoral immunity in albino rats. Health and Education in the 21st century. 2013;1-4(15):227-9. [Russian].
- Havreliuk SV, Chykina IV. Effect of chronic immobilisation stress on the development of endothelial dysfunction in rats. Fiziol Zh. 2017;63(2):56-64. [Ukrainian].
CrossRef
- Timchenko OI, Omelchenko EM, Belitskaya EN, Glavatskaya VI, Golovkova TA, Vigovskaya TA, Shumilo AM, Tsyiguleva OM. Heavy metals (lead, cadmium, mercury) as environmental pollutants in Ukraine. Kyiv; 2008:77. [Russian].
- Fedorenko VI. The substantiation of the maximum daily permissible doses of the lead and cadmium in everyday diet. Med Perspect. 2019;24(1):73-80. [Ukrainian].
CrossRef
International program on chemical safety environmental health criteria. 134. Cadmium: World Health Organization Geneva, 1992. Available from: http:.www.inchem. org. documents. ehc. ehc. ehc134.htm
Robin A Bernhoft. Cadmium toxicity and treatment. Sci World J. 2013;2013(ID 394652):[7 pages]. Available from: http:.dx.doi.org. 10.1155. 2013. 394652
- Ismaylova KhYu, Agaev TM, Semenova TP. Individual pecularities of the behavior: (monoaminergic mechanisms). Baku: Nurlan; 2007:228. [Russian].
- Bahshalieva AJa. Features of development of depressive state in rats with different individual-typological status. Neurophysiology. 2010;42(2):153-61. [Russian].
CrossRef
- Karpovskyi VV, Trokoz VO, Karpovskyi VI, Danchuk OV, Postoi RV. Cortical regulation of exchange of lipids in pigs. Kyiv: DDP «Ekspo-Druk»; 2017. [Ukrainian].
- Karpovskiy VI, Zhurenko OV, Trokoz VO, Postoy RV, Sysyuk YuO, Kravchenko-Dovha YuV, Landarenko LS. Cortico-vegetative relations in the regulation of physiological functions in cows. Scientific Messenger of LNUVMBT named after S.Z. Gzhytskyj. 2016;18(1Pt 2):64-9. [Ukrainian].
- Fedorenko YuV. Biochemical parameters and adaptation of white rats with active and passive type of behavior under immobilization stress. Med Perspect. 2016;21(2):13-9. [Ukrainian].
CrossRef
Khopta NS, Ersteniuk AM. Metabolic changes in the bone tissue of animals under experimental cadmiosis. Scientific J. "Science Rise: Biological Science". 2018;5(14):31-5. [Ukrainian]. Available from: DOI: 10.15587. 2519- 8025.2018.147090
CrossRef
- Wu Si, Kot Yu, Kot K, Morse R, Doran O, Hamdallah Amjad, Persky E. Total metabolism and oxidative stress parameters in rats at long-term exposure to low concentrations of Cd2+. J V.N. Karazin Kharkiv Nat Univ. Ser: Biology. 2017;(29):182-3. [Russian].
- Panin LE. Biochemical mechanisms of stress. Novosibirsk: Nauka; 1983:233. [Russian].
- Melnikova NM, Shepelova IA, Derkach EA, Motornyuk AV. Biochemical characteristic of blood of rabbits, poisoned with cadmium sulfate. Scientific Messenger of LNUVMBT named after S.Z. Gzhytskyj. 2009;1(Pt 2):227-30. [Ukrainian].
- Kalinin IV. Efficiency of aluminosilicates for reducing heavy metals in rat tissues. Fiziol Zh. 2017;63(5):41-8. [Ukrainian].
CrossRef
|
|
|
|
|
|
|
© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2024.
|
|