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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2020; 66(1): 10-17

INFLUENCE OF TWO OPTIONS OF DOSED ALIMENTARY DEPRIVATION ON THE INORGANIC AND ORGANIC BONE TISSUE MATRIX YA YOUNG RATS

I.G. Litovka1, S.P. Vesselsky2, S.Magomedov3, C.P. Bogomolets4, R.V. Yanko1, V.Ya. Berezovsky1

  1. Bogomolets Institute of Physiology National Academy of Sciences of Ukraine
  2. Taras Shevchenko Kyiv National University
  3. The Institute of Traumatology and Orthopaedics of NAMSU of Ukraine
  4. Bogomolets National Medical University, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz66.01.010


Abstract

The effect of 28-day 30% restriction of nutrition and periodic fasting (after every 2 days) on inorganic and organic bone tissue of 3-month-old Wistar male rats was studied. All results were compared with the control group (digestive diet). The tendency to a decrease the concentration of calcium and magnesium in the diaphysis of the femur in rats of both experimental groups was revealed. The content of phosphorus in the bone tissue of rats under the 28-day 30% restriction of nutrition probably decreased by 26.1%. Alkaline phosphatase activity decreased by 37.3% in serum and by 31.1% in bone tissue. During the period of daily fasting, this indicator decreased by 36.7% in serum and by 30.2% in bone tissue. The activity of acid phosphatase in serum decreased in rats of both groups by 36.2 and 37.5%, and in bone tissue by 47.4 and 37.7%, respectively. The concentration of glycosaminoglycans increased by 101.7% in the serum of rats under a 28-day 30% restriction of nutrition and by 35.6% under conditions of periodic fasting. A significant decrease in the concentration of uronic acids in the bone tissue of rats was found under conditions of 28-day 30% restriction of nutrition. In both experimental groups the concentration of free amino acids, lysine, cystine, alanine and methionine, which are directly involved in the synthesis of bone collagen, increased. Thus, under 30% restriction of nutrition and periodic starvation in early ontogeny (puberty) lead to adverse changes in biochemical markers of metabolism of inorganic and organic matrix and inhibit bone remodeling.

Keywords: bone tissue; markers of bone remodeling; dietary restriction

Full text: (PDF, in Ukrainian)

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