INFLUENCE OF TWO OPTIONS OF DOSED ALIMENTARY DEPRIVATION ON THE INORGANIC AND ORGANIC BONE TISSUE MATRIX YA YOUNG RATS
I.G. Litovka1, S.P. Vesselsky2, S.Magomedov3, C.P. Bogomolets4, R.V. Yanko1, V.Ya. Berezovsky1
- Bogomolets Institute of Physiology National Academy of Sciences of Ukraine
- Taras Shevchenko Kyiv National University
- The Institute of Traumatology and Orthopaedics of NAMSU of Ukraine
- Bogomolets National Medical University, Kyiv, Ukraine
The effect of 28-day 30% restriction of nutrition and periodic
fasting (after every 2 days) on inorganic and organic bone
tissue of 3-month-old Wistar male rats was studied. All results
were compared with the control group (digestive diet).
The tendency to a decrease the concentration of calcium and
magnesium in the diaphysis of the femur in rats of both experimental
groups was revealed. The content of phosphorus in the
bone tissue of rats under the 28-day 30% restriction of nutrition
probably decreased by 26.1%. Alkaline phosphatase activity
decreased by 37.3% in serum and by 31.1% in bone tissue.
During the period of daily fasting, this indicator decreased by
36.7% in serum and by 30.2% in bone tissue. The activity of
acid phosphatase in serum decreased in rats of both groups by
36.2 and 37.5%, and in bone tissue by 47.4 and 37.7%, respectively.
The concentration of glycosaminoglycans increased by
101.7% in the serum of rats under a 28-day 30% restriction of
nutrition and by 35.6% under conditions of periodic fasting.
A significant decrease in the concentration of uronic acids in
the bone tissue of rats was found under conditions of 28-day
30% restriction of nutrition. In both experimental groups the
concentration of free amino acids, lysine, cystine, alanine
and methionine, which are directly involved in the synthesis
of bone collagen, increased. Thus, under 30% restriction of nutrition and periodic starvation in early ontogeny (puberty)
lead to adverse changes in biochemical markers of metabolism
of inorganic and organic matrix and inhibit bone remodeling.
bone tissue; markers of bone remodeling; dietary restriction
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