Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2020; 66(1): 10-17


INFLUENCE OF TWO OPTIONS OF DOSED ALIMENTARY DEPRIVATION ON THE INORGANIC AND ORGANIC BONE TISSUE MATRIX YA YOUNG RATS

I.G. Litovka1, S.P. Vesselsky2, S.Magomedov3, C.P. Bogomolets4, R.V. Yanko1, V.Ya. Berezovsky1

  1. Bogomolets Institute of Physiology National Academy of Sciences of Ukraine
  2. Taras Shevchenko Kyiv National University
  3. The Institute of Traumatology and Orthopaedics of NAMSU of Ukraine
  4. Bogomolets National Medical University, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz66.01.010

Abstract

The effect of 28-day 30% restriction of nutrition and periodic fasting (after every 2 days) on inorganic and organic bone tissue of 3-month-old Wistar male rats was studied. All results were compared with the control group (digestive diet). The tendency to a decrease the concentration of calcium and magnesium in the diaphysis of the femur in rats of both experimental groups was revealed. The content of phosphorus in the bone tissue of rats under the 28-day 30% restriction of nutrition probably decreased by 26.1%. Alkaline phosphatase activity decreased by 37.3% in serum and by 31.1% in bone tissue. During the period of daily fasting, this indicator decreased by 36.7% in serum and by 30.2% in bone tissue. The activity of acid phosphatase in serum decreased in rats of both groups by 36.2 and 37.5%, and in bone tissue by 47.4 and 37.7%, respectively. The concentration of glycosaminoglycans increased by 101.7% in the serum of rats under a 28-day 30% restriction of nutrition and by 35.6% under conditions of periodic fasting. A significant decrease in the concentration of uronic acids in the bone tissue of rats was found under conditions of 28-day 30% restriction of nutrition. In both experimental groups the concentration of free amino acids, lysine, cystine, alanine and methionine, which are directly involved in the synthesis of bone collagen, increased. Thus, under 30% restriction of nutrition and periodic starvation in early ontogeny (puberty) lead to adverse changes in biochemical markers of metabolism of inorganic and organic matrix and inhibit bone remodeling.

Keywords: bone tissue; markers of bone remodeling; dietary restriction

References

  1. Villareal DT, Fontana L, Das SK, et.al. Effect of two-year caloric restriction on bone metabolism and bone mineral density in non-obese younger adults: a randomized clinical trial. J Bone Mineral Res. 2016;31(1):40-51. CrossRef PubMed PubMedCentral
  2.  
  3. Zibellini J, Seimon RV, Lee CM, et al. Does diet-induced weight loss lead to bone loss in overweight or obese adults? A systematic review and meta-analysis of clinical trials. J Bone Mineral Res. 2015;30(12): 2168-78. CrossRef PubMed
  4.  
  5. Guedes PMSG, Zamarioli A, Botega II, et.al. Undernutrition impairs the quality of growth plate and trabecular and cortical bones in growing rats1. Acta Cir b Bras. 2019;34(3):e201900301. CrossRef PubMed PubMedCentral
  6.  
  7. McCay CM, Maynard LA, Sperling G, Osgood HS. Nutritional requirements during the latter half of life: one figure. J Nutrition. 1941;21(Issue 1):45-60. CrossRef  
  8. Reeve J. PTH: a future role in manage men to fosteoporosis? J Bone Mineralres. 1996;11(4):440-5. CrossRef PubMed
  9.  
  10. Pop LC, Sukumar D, Tomaino K. Moderate weight loss in obese and overweight men preserves bone quality. Am J Clin Nutrition. 2015;101(3):659-67. CrossRef PubMed PubMedCentral
  11.  
  12. Mattson MP, Longo VD, Harvie M. Impact of intermittent fasting on health and disease processes. Ageing Res Rev. 2017;39:46-58. CrossRef PubMed PubMedCentral
  13.  
  14. Hisatomi Y, Kugino K. Changes in bone density and bone quality caused by single fasting for 96 hours in rats. Peer J. 2019;6: e6161. CrossRef PubMed PubMedCentral
  15.  
  16. Povoroznyuk VV, Hopkalova IV, Grigorieva NV. Features of changes in bone mineral density in white Wistar rats depending on age and sex. Probl Aging and Longevity. 2011;20(4):391-401. [Russian].
  17.  
  18. Klyatskin SA, Lifshitz RI. Determination of glycosaminoglycans by ortsin method in patients' blood. Lab Work. 1989;10:51-3. [Russian].
  19.  
  20. Leontiev VK, Petrovich YA. Biochemical methods in clinical and experimental dentistry. Omsk. 1976. [Russian].
  21.  
  22. Sharaev PN, Strelkov NS, Guncha VV, Sosulina LL. Determination of hyaluronidase activity in biological fluids. Clin Lab Diagnost. 1996;3:21-2. [Russian].
  23.  
  24. Veselskiy SP, Lyaschenko PS, Kostenko SI, et.al. Way of sample preparation of bioliquids detecting in lipids determination. Pryroda. Ukrainian Patent 33564A, 2001, Feb.
  25.  
  26. Kaznacheeva AI, Zlydnev NZ. The content of free aminoacids in healthy blood plasma, erythrocytes and urine. Lab Work.1976;8:479-80. [Russian].
  27.  
  28. Zainabadi K, Liu CJ, Caldwell ALM, Guarente L. SIRT1 is a positive regulator of in vivo bone mass and a therapeutic target for osteoporosis. PLoS One. 2017;12(9):1-12. CrossRef PubMed PubMedCentral
  29.  
  30. Sørensen KU, Kruger MC, Hansen-Møller J, Poulsen HD. Bone biochemical markers for assessment of bone responses to differentiated phosphorus supply in growingfinishing pigs. J Animal Sci. 2018;96(11):4693-703. CrossRef PubMed
  31.  
  32. WadaT, NakashimaT, Hiroshi N, Penninger JM. RANKLRANK signaling in osteoclastogenesis and bone disease. Trends Mol Med. 2006;12(1):17-25. CrossRef PubMed
  33.  
  34. Kenkre JS, Bassett JHD. The bone remodelling cycle. Ann Clin Biochem. 2018;55:308-27. CrossRef PubMed
  35.  
  36. Henriksen K, Bollerslev J, Everts V, Karsdal MA. Osteoclast activity and subtypes as a function of physiology and pathology. Implications for future treatments of osteoporosis. Endocrin Rev. 2011;32(1):31-63. CrossRef PubMed
  37.  
  38. Shlikova VO, Levanda OM. Income, nutritional conditions, and life span of the population: A comparative analysis of Ukraine and EU. Countries Demography and Soc Econ. 2018;1(32):140-52. CrossRef  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2020.