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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2020; 66(1): 3-9

Neuroprotective effects of a new nootropic agent Mitochondrin-2

K.A. Dronska1, M.V. Vitushynska1, Y.I. Chernyk1, A.N. Makarenko2, N.P. Matiytsiv1

  1. Department of Genetics and Biotechnology, Ivan Franko National University of Lviv, Lviv, Ukraine
  2. Department of Psychology, Interregional Academy of Personnel Management, Kyiv, Ukraine


Since neurodegenerative diseases are incurable, it is important to study experimental neuroprotective preparation on model organisms. In this work, we studied the neuropeptide Mitochondrin-2 (M-2) effect on Drosophila neurodegenerative models of brain pathology induced by functional knockdown of swiss cheese (sws) and Superoxide dismutase 1 (Sod-1) genes in gliocytes. We found that, after using M-2, brain zone degeneration decreased and markers of oxidative stress (conjugates diene CD and thiobarbituric acid reactive substances TBARS) reached the control level. However, we did not observe any improvement in life span or locomotor activity. This suggests a certain neurotrophic and antioxidant effect of M-2, especially for glial cells, but its effect on the protection of neurons function remains unclear. Further research is needed for effect mechanism of peptide neuroprotectors.

Keywords: Drosophila melanogaster; neurodegeneration; swiss cheese gene; Sod-1 gene; neuropeptides


  1. Pandey U, Nichols CD. Human disease models in Drosophila melanogaster and the role of the fly in therapeutic drug discovery. Pharmacol Rev. 2011; 63(2):411-36. CrossRef PubMed PubMedCentral
  3. Debattisti V, Scorrano L. D. melanogaster and mitochondria and neurodegeneration: small model organism, big discoveries. Mol Cell Neurosci. 2013 Jul;55:77-86. CrossRef PubMed
  5. Mohylyak I, Chernyk Ya. Functioning of glia and neurodegeneration in Drosophila melanogaster. Tsitol Genet. 2017;51:202-13. CrossRef  
  6. Nusbaum M, Blitz D. Neuropeptide modulation of microcircuits. Curr Opin Neurobiol. 2012;22:592-601. CrossRef PubMed PubMedCentral
  8. Nässel D. Neuropeptide signaling near and far: how localized and timedis the action of neuropeptides in brain circuits in brain circuits? Invert Neurosci. 2009;9:57-75. CrossRef PubMed
  10. Chad M, Artymovych N, Makarenko O, Matiytsiv N. The influence of Mitohondrin-2 on the development of brain tissue in old flies with changed function of swiss cheese gene. Neurophysiology. 2014;46:519-24. CrossRef  
  11. Makarenko A N, Kul'chikov AE, Morozov SG, et al. Medicinal Preparation for Treatment of Hypoxic and Toxic Mitochondrial Abnormalities and the Technique for Its Synthesis. Inventors certificate of Russia No. 2405. 558. Byull. 2010 Dec 10;23. [Russian].
  13. Ashburner M. Drosophila: a Laboratory Manual. New York: Cold Spring Harbor Lab; 1989.
  15. Heisenberg M, Bohl K. Isolation of anatomical brain mutants of Drosophila by histological means. Naturforsch. 1979;34:143-7. CrossRef  
  16. Helfrich-Forster C. Immunohistochemistry in Drosophila: sections and whole mounts. Methods Mol Biol. 2007;362:533-47. CrossRef PubMed
  18. Sharma SK, Babitch A. Application of Bradford's protein assay to chick brain subcellular fractions. J Biochem Biophys Methods. 1980;2(4):247-50. CrossRef  
  19. Jazwinski SM. Longevity, genes, and aging. Science. 1996 Jul 5;273(5271):54-9. CrossRef PubMed
  21. Edvards TN, Meinertzhagen IA. The functional organization of glia in the adult brain of Drosophila and other insects. Prog Neurobiol. 2010;90(4):471-97. CrossRef PubMed PubMedCentral
  23. Vitushynska MV, Matiytsiv NP, Chernyk YP. Influence of tissue-specific superoxide dismutase genes expression in brain cells on Drosophila melanogaster sensitivity to oxidative stress and viability. Tsitol Genet. 2015;49(2): 21-8. CrossRef PubMed
  25. Matiytsiv NP, Mohylyak II, Truhs OI, Chernyk YI. Motor activity of Drosophila melanogaster neurodegenerative mutants. Odesa Nat Univ Herald. 2013;2(31):70-6. [Ukrainian]. CrossRef  
  26. Kretzschmar D, Hasan G, Sharma S, Heisenberg M. The swiss cheese mutant cause glial hyperwrapping and brain degeneration in Drosophila. J Neurosci. 1997 Oct 1;17(19):7425-32. CrossRef PubMed PubMedCentral
  28. Matiytsiv N, Makarenko O, Chernyk Ya. Cerebral and Mitohondrine-2 effects on the viability and dynamics of degenerative changes in the Drosophila melanogaster mutants brain tissue. Rus J Biopharmaceuticals. 2012;4:54-56. [Russian].
  30. Ayala A, Muñoz M, Argüelles S. Lipid Peroxidation: Production. Metabolism, and Signaling Mechanisms of Malondialdehyde and 4-Hydroxy-2-Nonenal. Oxid Med Cell Longev. 2014:360438. CrossRef PubMed PubMedCentral

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