THE FUNCTIONING OF LARGE CONDUCTANCE CATIONIC CHANNELS IN THE NUCLEAR MEMBRANE OF CARDIOMYOCYTES AND CEREBELLAR PURKINJE NEURONS UNDER THE INFLUENCE OF NICOTINIC CHOLINORECEPTOR MODULATORS
A.B. Kotliarova, O.A. Kotyk, I.V. Yuryshynets, S.M. Marchenko
O.O. Bogomolets Institute of Physiology, NAS of Ukraine
DOI: https://doi.org/10.15407/fz65.06.030
Abstract
Large conductance cationic channels (LCC-channels) are
highly expressed in the nuclear envelope of neurons and
cardiomyocytes. Using electrophysiological registration
of ion currents through the inner nuclear membrane of
cardiomyocytes and cerebellar Purkinje neurons, we have
tested the effect of n-choline receptor modulators (activators
and inhibitors) on LCC-channels. Among the substances
tested, rocuronium- and pipecuronium-bromide and nicotine
proved to be effective. The pharmacological sensitivity of
LCC-channels in Purkinje neuron and cardiomyocyte nuclear
membranes was almost identical, indicating existence of a
single population of these channels in the tissues studied. At
a concentration of 0.2 mM nicotine reduced in half the current
through LCC-channels, while rocuronium- and pipecuroniumbromide
at the same concentration inhibited these channels
by 7 and 12 % respectively. Therefore, among the listed
substances, only the effect of nicotine on LCC-channels was
close to the effect of previously studied tubocurarine, that’s
why tubocurarine and nicotine remain the most promising
tools for further investigation of the physiological role of
these channels.
Keywords:
nuclear membrane, LCC-channels, n-cholinoreceptor inhibitors, agonists of n-cholinoreceptors, pipecuronium bromide, rocuronium bromide, hexamethonium, methyllycaconitine (MLA), α-conotoxin PeIA, PNU 282987, nicotine
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