Influence of different types of activity of muscles of the upper limb on the frequency and amplitude of physiological tremor in 40 females of 18-19 years old was investigated. Tests were carried out in two positions of the brush (palm down and palm rib down) as well as in three positions (at rest, in postural condition, and during loading). In the position of the brush with the palm down in the rest, the frequency of the physiological tremor was along the x-axis 7.74±0.28 Hz, y-axis - 7.38±0.34 Hz, z-axis - 7.47±0.22 Hz, whereas the amplitude was 0.12±0.01, 0.22±0.02 and 0.24±0.02 mm, respectively. As a result of displacement of the brush to the postural condition, the frequency of physiological tremor increased along the z-axis, which coincides with the force of gravity, to 8.10±0.18 Hz, and the amplitude increased along three axes by 33%, 90%, and 216%, respectively. Applying the load 100 and 200 g led to a decrease in the frequency and amplitude of the physiological tremor in this position of the brush. In the position of the brush with a palm rib down, the frequency data also varied only along the axis, which coincides with the force of gravity, whereas changes in the amplitude were not detected, which is explained by the features of the radial-wrist joint structure. The results could be explained by different activity and alternation of the muscles involved in the work when maintaining the brush in a given position. Consequently, in the state of rest, regardless of the position of the brush, the muscle tone supporting the radial-wrist joint practically does not affect the frequency and amplitude of the physiological tremor. When transferring the limb to the postural condition, in both cases, statistically significant changes in the frequency of the physiological tremor are observed only along the axis, which coincides with the force of gravity. The tremor amplitude also depends on the load of the limb, but only in the position of the hand with the palm down.