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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2017; 63(3): 16-23


ACTIVATION OF ENDOGENOUS HYDROGEN SULFIDE SYNTHESIS INHIBITS MITOCHONDRIAL PERMEABILITY TRANSITION PORE OPENING AND RESTORES CONSTITUTIVE NO-SYNTHASE COUPLING IN OLD RAT HEART

L.A. Mys, N.A. Strutynska, V.R. Strutynskyi, V.F. Sagach

    O.O. Bogomoletz Institute of Physiology National Academy of Science of Ukraine, Kyiv
DOI: https://doi.org/10.15407/fz63.03.016

Abstract

cofactor pyridoxal-5-phosphate on the sensitivity of mitochondrial permeability transition pore (MPTP) opening to Ca2+ as natural inducer in old rat heart, as well as the H2S content and oxidative and nitrosative stress indicators in heart mitochondria. It was shown that in old rats oxidative and nitrosative stress developed which accompanied by a decrease of the constitutive Ca2+-dependent NO-synthase (cNOS) coupling index. P5P application inhibited Ca2+-induced MPTP opening by reducing the sensitivity to the inductor in old rat heart. H2S synthesis modulator application led to increase hydrogen sulfide values in 4,2 times and promoted to reduce the .O2- generation speed in 3,5 times in comparison with these indicators in old animals. An important consequence of stimulating of hydrogen sulfide endogenous synthesis in aging was the increase activity of cNOS in 1,9 times and decrease the activity of Ca2+-independent inducible NO-synthase (iNOS) in 4,3 times in heart mitochondria. Under these conditions in organelles cNOS coupling index increased in 8,7 times relative values in old animals. Thus, H2S acts as a regulator of MPTP opening and content NO, increasing activity cNOS and recoverability of enzyme coupled state, indicating the importance of this gaseous transmitter in the pathology of the cardiovascular system.

Keywords: hydrogen sulfide; cNOS coupling; oxidative and nitrosative stress; aging; mitochondria; heart; rats.

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