OMEGA-3 POLYUNSATURATED FATTY ACIDS NORMALIZE THE FUNCTION OF MITOCHONDRIA, ACTIVITY OF ENZYMES OF PROOXIDANT-ANTIOXIDANT SYSTEM AND THE EXPRESSION OF CYTOCHROME Р450 2Е1 AFTER ISOPROTERENOLINDUCED MYOCARDIAL INJURY
O.S. Panasiuk, A.M. Shysh, O.O. Moibenko
O.O.Bogomoletz Institute of Physiology National Academy
of Sciences of Ukraine, Kyiv
DOI: https://doi.org/10.15407/fz62.02.064
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Abstract
We have studied the influence of dietary ω-3 polyunsaturated
fatty acids (ω-3 PUFA) on the functioning of subsarcolemmal
and interfibrillar mitochondrial fractions of rat myocardium,
changes in expression of cytochrome P450 (CYP2E1) and the
activity of enzymes of prooxidant-antioxidant system after
isoproterenol-induced myocardial injury. It has been found
that in vivo administration of ω-3 PUFA (Epadol 0.1 ml/100
gr of weight for 4 weeks) significantly reduced the swelling
of subsarcolemmal and interfibrillar mitochondrial fractions
by 65.52% 54.84% respectively, pointing for a decrease of
damage of the mitochondrial function evoked by in vivo
administration of isoproterenol. In vivo administration of
ω-3 PUFAs prevents a decrease in the activity of antioxidant
enzymes catalase and superoxide dismutase (2.65 and 7.1-
fold, respectively) after isoproterenol-induced myocardial
injury. We suggest that the development of oxidative stress
after isoproterenol-induced myocardial injury can be caused
by a significant increase in the expression of cytochrome P450
2E1 (73.3%), and administration of ω-3 PUFAs prevents such
changes.
Keywords:
mitochondria; subsarcolemmal; interfibrillar; isoproterenol; ω-3 polyunsatureted fatty acid; malondialdehyde; superoxide dismutase; catalase; cytochrome Р450 2Е1.
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