EFFECT OF PEPTIDOGLYCANE OF STAPHYLOCOCCUS AUREUS CELL WALL ON THE MECHANISM OF REGULATION OF CONTRACTILE ACTIVITY OF RAT MYOMETRIUM BY ADENYLATE CYCLASE SYSTEM
L.S. Nasibyan, I.B. Philyppov
O.O.Bogomoletz Institute of Physiology of the National
Academy of Sciences of Ukraine, Kyiv.
DOI: https://doi.org/10.15407/fz62.01.025
Abstract
The revue deals with the role of each component of adenylate
cyclase regulatory system in the rat myometrial contractile
activity modulation by the peptidoglycane of Staphylococcus
aureus. Noradrenalin and salbutamol were used to investigate
peptidoglycane impact on the myometrial β-adrenergic
receptors. It was shown that inhibited by these substances
myometrial contractility increased to the initial level after peptidoglycane
application. The same effect we observed under
the cAMP level elevation by forscolin. Peptidoglycan’
s ability
to strengthen contractions was inhibited by the 8-brom-cAMP
and papaverine application. Stimulation of Gs-protein by the
cholera toxin didn’t influence on the peptidoglycane effect
while the blocking of Gi/o-protein by the pertussis toxin caused
stopping it’s manifestation. We concluded that the modulating
effect of peptidoglycane implemented via Gi/o-protein activation,
which causes adenilatcyclase desensitization.
Keywords:
peptidoglycane; Staphylococcus aureus; myometrium; contractility; β-adrenoceptors; adenylate cyclase regulatory system; G-protein.
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