THE EFFECT OF ADAPTIVE HORMONES ON THE SECRETORY ACTIVITY OF INSULAR APPARATUS AND THE ZINC CONTENT IN IT AND IN THE CELLS OF HIPPOCAMPUS, PINEAL AND THYMUS GLANDS IN RATS WITH ALLOXANINDUCED DIABETES
Zaporizhzhya National University, Zaporizhzhya, Ukraine
The influence of adaptive hormones (insulin, adrenaline and
prednisolone) on the pancreatic islets functional state was
studied in experiments on rats with alloxan-induced diabetes.
It was estimated by the concentration of glucose in the blood,
insulin and zinc in B-cells. The presence of cytochemically
determined zinc in the hippocampus neurons, pinealocytes
and thymic epithelial cells (TEC) has allowed a comparative
research of changes of this metal content in the hippocampus,
pineal, thymus and pancreas glands. For determination of zinc
content in the cells, a highly selective cytochemical reaction
of 8-(p-toluenesulfonylamino)-quinoline was used. It has been
found that alloxan not only caused the increase of glycemia,
insulin deficiency in the pancreatic B cells (87%) but also
a development the zinc deficit (48-59% ) in hippocampus,
epiphysis, thymus and pancreatic islets. The opposite changes
in blood and cells were observed after insulin injection. It can
be explained by inhibition of secretory processes. Adrenaline
and prednisolone introduction also induced an increase in
zinc content in pancreatic islets, hippocampus (27-32%),
epiphysis (27-35%), thymus (25-33%), accompanied by a
doubled glucose concentration in blood. The insulin deficit in
B-cell (56-81%) as well as a zinc deficit in the hippocampal
neurons (5-25%), pinealocytes (19-38%), TEC (32-42%)
and B-insulocytes (24-41%) were less marked in case of the
adaptive hormones prescription to the animals with diabetes.
The correlation analysis of changes in zinc content showed
a link between the hippocampus neurons, the cells of pineal
and the thymus glands, B-insulocytes, suggesting the existence
of hippocampal-epiphyseal-thymic system of regulation of
insular apparatus function.
alloxan, epiphysis, hippocampus, pancreatic islets,thymus, zinc.
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