THE ASSOCIATION OF PROMOTER GENE MATRIX METALLOPROTEINASE-9 POLYMORPHISM C-1562→T WITH THE RISK OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE IN MINERS
A.V. Basanets, L.V. Dolinchuk, T.A. Andrushchenko
SI “Institute of Occupational Health of NAMS of Ukraine”,Kyiv, Ukraine
We analyzed genetic markers of genetic susceptibility that
lead to development of chronic obstructive pulmonary disease
(COPD) of occupational etiology. Polymerase chain reaction
(PCR) followed by restriction fragment length polymorphism
analysis were performed to detect a point mutation at the
promoter of C-1562ЃЁT (rs3918242) of the matrix metalloproteinase-
9 (MMP-9) gene. The study comprised 72 miners
with COPD, and 79 miners without respiratory system pathology.
The frequency of allocation for genotypes MMP-9 gene
polymorphism C-1562ЃЁT was similar to Caucasian population.
The study established the association between the minor
T-allele (Odds Ratio (OR) = 1.89; 95% confidence interval
(CI): 0.98-3.66) and TT (OR = 2.18; 95% CI: 1.83-2.60) and
CT (OR = 1.21; 95% CI: 0.56-2.66) genotypes with the risk
of COPD in miners of main underground occupations. Presence
in the genome dominant C-allele (OR = 0.53; 95% CI:
1.02-0.27) and CC genotype (OR = 0.60; 95% CI: 0.28 - 1.28)
determine the relative protective role in risk to COPD among
respondents of the studied group. The results of research opens
new perspectives for improving measures of primary prevention
of COPD based on determining genetic predisposition to
chronic obstructive pulmonary disease, geneticpredisposition, miners of major underground occupations.
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