Українська English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2014; 60(6): 16-21

THE ASSOCIATION OF PROMOTER GENE MATRIX METALLOPROTEINASE-9 POLYMORPHISM C-1562→T WITH THE RISK OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE IN MINERS

A.V. Basanets, L.V. Dolinchuk, T.A. Andrushchenko

    SI “Institute of Occupational Health of NAMS of Ukraine”,Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz60.06.016


Abstract

We analyzed genetic markers of genetic susceptibility that lead to development of chronic obstructive pulmonary disease (COPD) of occupational etiology. Polymerase chain reaction (PCR) followed by restriction fragment length polymorphism analysis were performed to detect a point mutation at the promoter of C-1562ЃЁT (rs3918242) of the matrix metalloproteinase- 9 (MMP-9) gene. The study comprised 72 miners with COPD, and 79 miners without respiratory system pathology. The frequency of allocation for genotypes MMP-9 gene polymorphism C-1562ЃЁT was similar to Caucasian population. The study established the association between the minor T-allele (Odds Ratio (OR) = 1.89; 95% confidence interval (CI): 0.98-3.66) and TT (OR = 2.18; 95% CI: 1.83-2.60) and CT (OR = 1.21; 95% CI: 0.56-2.66) genotypes with the risk of COPD in miners of main underground occupations. Presence in the genome dominant C-allele (OR = 0.53; 95% CI: 1.02-0.27) and CC genotype (OR = 0.60; 95% CI: 0.28 - 1.28) determine the relative protective role in risk to COPD among respondents of the studied group. The results of research opens new perspectives for improving measures of primary prevention of COPD based on determining genetic predisposition to COPD development.

Keywords: chronic obstructive pulmonary disease, geneticpredisposition, miners of major underground occupations.

Full text: (PDF, in Ukrainian)

References

  1. Lopez AD, Shibuya K, Rao C, Mathers CD, Hansell AL,Held LS, et al. Chronic obstructive pul monary disease:current burden and future projections. Eur Respir J.2006;27:397-412. CrossRef PubMed
    2.  
  2. Mathers CD, Loncar D. Projections of global mortality and burden of disease from 2002 to 2030. PLoS Med. 2006;3(11):e442. CrossRef PubMed PubMedCentral
    3.  
  3. Trupin L, Earnest G, San Pedro M, Balmes JR, Eisner MD, Yelin E, et al.The occupational burden of chronic obstructive pulmonary disease. Eur Respir J. 2003. 22(3). 462-69. CrossRef PubMed
    4.  
  4. Buist AS, McBurnie MA, Vollmer WM, Gillespie S, Bur- ney P, Mannino DM, et al. International variation in the prevalence of COPD (the BOLD Study): a populationbased prevalence study. Lancet. 2007;370:741-50. CrossRef  
  5. Kundiev YI, Nahorna AM, Basanets AV. Occupational diseases that arise due to dust. In: Kundiev YI, editors. Professional health in Ukraine. Kyiv: Avicena;2006. p.99-124.
    6.  
  6. Elkington PTG, Friedland JS. Matrix metalloproteinases in destructive pulmonary pathology. Thorax. 2006. 61. 259-66. CrossRef PubMed PubMedCentral
    7.  
  7. Ohnishi K, Takagi M, Kurokawa Y, Satomi S, Konttinen YT. Matrix metalloproteinase-mediated extracellular matrix protein degradation in human pulmonary emphysema. Lab Invest. 1998. 78. 1077-87. PubMed
    8.  
  8. Zhang B, Henney A, Eriksson P, Hamsten A, Watkins H, Ye S. Genetic variation at the matrix metalloproteinase- 9 locus on chromosome 20q12.2-13.1. Hum Genet. 1999. 105. 418-23. CrossRef PubMed
    9.  
  9. Global Initiative for Chronic Obstructive Lung Disease. Global Strategy or the Diagnosis, Management and Prevention of Chronic ObstructiveLung Disease. NHLBI/ WHO workshop report. Last update 2011. Available from: http://www.goldcopd.com.
    10.  
  10. Jones GT, Phillips VL, Harris EL, Rossaak JI, van Rij AM. Functional matrix metalloproteinase-9 polymorphism (C-1562T) associated with abdominal aortic aneurysm. J Vasc Surg. 2003. 38(6):1363-7. CrossRef  
  11. National Center for Biotechnology Information Available from: http:http:.www.ncbi.nlm.nih.gov/projects/SNP/.
    12.  
  12. Zhang B, Ye S, Herrmann SM, Eriksson P, de Maat M, Evans A, et al. Functional polymorphism in the regulatory region of gelatinase B gene in relation to severity of coronary atherosclerosis. Circulation.1999. 99. 1788-94. CrossRef PubMed
    13.  
  13. Minematsu N, Nakamura H, Tateno H, Nakajima T, Yamaguchi K. Genetic polymorphism in matrix metalloproteinase-9 and pulmonary emphysema. Biochem Biophys Res Commun. 2001. 289(1). 116-9. CrossRef PubMed
    14.  
  14. Joos L, He JQ, Shepherdson MB, Connett JE, Anthonisen NR, Pare PD, et al. The role of matrix metalloproteinase polymorphisms in the rate of decline in lung function. Hum Mol Genet. 2003.12.803-4.
    15.  

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2025.