THE ASSOCIATION OF PROMOTER GENE MATRIX METALLOPROTEINASE-9 POLYMORPHISM C-1562→T WITH THE RISK OF CHRONIC OBSTRUCTIVE PULMONARY DISEASE IN MINERS
A.V. Basanets, L.V. Dolinchuk, T.A. Andrushchenko
SI “Institute of Occupational Health of NAMS of Ukraine”,Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz60.06.016
Abstract
We analyzed genetic markers of genetic susceptibility that
lead to development of chronic obstructive pulmonary disease
(COPD) of occupational etiology. Polymerase chain reaction
(PCR) followed by restriction fragment length polymorphism
analysis were performed to detect a point mutation at the
promoter of C-1562ЃЁT (rs3918242) of the matrix metalloproteinase-
9 (MMP-9) gene. The study comprised 72 miners
with COPD, and 79 miners without respiratory system pathology.
The frequency of allocation for genotypes MMP-9 gene
polymorphism C-1562ЃЁT was similar to Caucasian population.
The study established the association between the minor
T-allele (Odds Ratio (OR) = 1.89; 95% confidence interval
(CI): 0.98-3.66) and TT (OR = 2.18; 95% CI: 1.83-2.60) and
CT (OR = 1.21; 95% CI: 0.56-2.66) genotypes with the risk
of COPD in miners of main underground occupations. Presence
in the genome dominant C-allele (OR = 0.53; 95% CI:
1.02-0.27) and CC genotype (OR = 0.60; 95% CI: 0.28 - 1.28)
determine the relative protective role in risk to COPD among
respondents of the studied group. The results of research opens
new perspectives for improving measures of primary prevention
of COPD based on determining genetic predisposition to
COPD development.
Keywords:
chronic obstructive pulmonary disease, geneticpredisposition, miners of major underground occupations.
References
- Lopez AD, Shibuya K, Rao C, Mathers CD, Hansell AL,Held LS, et al. Chronic obstructive pul monary disease:current burden and future projections. Eur Respir J.2006;27:397-412.
CrossRef
PubMed
- Mathers CD, Loncar D. Projections of global mortality and burden of disease from 2002 to 2030. PLoS Med. 2006;3(11):e442.
CrossRef
PubMed PubMedCentral
- Trupin L, Earnest G, San Pedro M, Balmes JR, Eisner MD, Yelin E, et al.The occupational burden of chronic obstructive pulmonary disease. Eur Respir J. 2003. 22(3). 462-69.
CrossRef
PubMed
- Buist AS, McBurnie MA, Vollmer WM, Gillespie S, Bur- ney P, Mannino DM, et al. International variation in the prevalence of COPD (the BOLD Study): a populationbased prevalence study. Lancet. 2007;370:741-50.
CrossRef
- Kundiev YI, Nahorna AM, Basanets AV. Occupational diseases that arise due to dust. In: Kundiev YI, editors. Professional health in Ukraine. Kyiv: Avicena;2006. p.99-124.
- Elkington PTG, Friedland JS. Matrix metalloproteinases in destructive pulmonary pathology. Thorax. 2006. 61. 259-66.
CrossRef
PubMed PubMedCentral
- Ohnishi K, Takagi M, Kurokawa Y, Satomi S, Konttinen YT. Matrix metalloproteinase-mediated extracellular matrix protein degradation in human pulmonary emphysema. Lab Invest. 1998. 78. 1077-87.
PubMed
- Zhang B, Henney A, Eriksson P, Hamsten A, Watkins H, Ye S. Genetic variation at the matrix metalloproteinase- 9 locus on chromosome 20q12.2-13.1. Hum Genet. 1999. 105. 418-23.
CrossRef
PubMed
- Global Initiative for Chronic Obstructive Lung Disease. Global Strategy or the Diagnosis, Management and Prevention of Chronic ObstructiveLung Disease. NHLBI/ WHO workshop report. Last update 2011. Available from: http://www.goldcopd.com.
- Jones GT, Phillips VL, Harris EL, Rossaak JI, van Rij AM. Functional matrix metalloproteinase-9 polymorphism (C-1562T) associated with abdominal aortic aneurysm. J Vasc Surg. 2003. 38(6):1363-7.
CrossRef
- National Center for Biotechnology Information Available from: http:http:.www.ncbi.nlm.nih.gov/projects/SNP/.
- Zhang B, Ye S, Herrmann SM, Eriksson P, de Maat M, Evans A, et al. Functional polymorphism in the regulatory region of gelatinase B gene in relation to severity of coronary atherosclerosis. Circulation.1999. 99. 1788-94.
CrossRef
PubMed
- Minematsu N, Nakamura H, Tateno H, Nakajima T, Yamaguchi K. Genetic polymorphism in matrix metalloproteinase-9 and pulmonary emphysema. Biochem Biophys Res Commun. 2001. 289(1). 116-9.
CrossRef
PubMed
- Joos L, He JQ, Shepherdson MB, Connett JE, Anthonisen NR, Pare PD, et al. The role of matrix metalloproteinase polymorphisms in the rate of decline in lung function. Hum Mol Genet. 2003.12.803-4.
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