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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2001; 47(2): 24-31


The effects of sex hormones on herg-potassiumchannels expressed in xenopus oocytes

V. M. Osipenko, V. E. Degtiar, V. G. Naidionov, Y. M. Shuba

    A. A. Bogomoletz Institute of PhysiologyNational Academy of Sciences of Ukraine;International Center of Molecular PhysiologyNational Academy of Sciences of Ukraine, Kiev


Abstract

The repolarisation phase of cardiac action potential is characterized by sexual dimorphism suggesting the role of sex steroid hormones in the regulation of K+ channels. Here we report on the effects of testosterone and 17?-estradiol on HERG-encoded K+ channels, expressed in Xenopus oocytes. At 1M concentration testosterone decreased the amplitude of HERG-directed IKr (rapid component of cardiac delayed rectifier K+ current) by 30 % within 30 min of exposure, while 17-estradiol had no effect. Testosterone did not alter the HERG channels kinetics, voltage-dependance, steady- state activation and inactivation properties suggesting that its action most probably involves the decrease of channels open probability and/or the level of their expression. The signaling pathways mediating testosterone-induced down-regulation of HERG channels in Xenopus oocytes remains to be elucidated. The effect of testosterone on HERG channels in Xenopus oocytes is opposite to what one might expect from shorter cardiac action potential duration and electrocardiographic QT-interval in males compared to females.

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