Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2013; 59(1): 32-39


Investigation of the changes in uterine myocytes size depending on contractile activity modulators by photon correlation spectroscopy

Danylovych IuV, Chunikhin OIu, Danylovych HV

    Kyiv Taras Shevchenko National University, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz59.01.032

Abstract

By using photon correlation spectroscopy the effect of contractile activity modulators of smooth muscle to the size (diameter) of rats uterine muscle cells was investigated. Cells were studied by using laser confocal microscopy as objects mostly oval or nearly oval form. The diameter of myocytes, assessed by photon correlation spectroscopy, was amounted 7-10 microm which is Consistent with the results obtained by using laser confocal microscopy. It is shown that the myoconstricted agents as Ca2+ ionophore A-23187 (10 microM) and K(+)-channels inhibitors (tetraethylammonium and 4-aminopyridine in a concentration of 1 mM) leading to decrease the diameter of the cells by 22%, 12% and 24% in average respectively. The ouabain, that reduces contractile activity of smooth muscle, leads to increase this parameter up 23% from control. Thus, decreasing/increasing the diameter of uterine myocytes correlates with their well known influence on myometrium constriction/relaxation.

Keywords: photon correlation spectroscopy, hydrodynamicdiameter, myocytes, myometrium, myoconctrictors, ouabain.

References

    Berezovskii V.A., Kolotilov N.N. Biofizicheskie harakteristiki tkanei cheloveka. Spravochnik. K.: Nauk. dumka, 1990. 224 s. Testuvannya zmin rozmiriv miotsitiv matki  
  1. Danilovich G.V., Danilovich Yu.V., Gorchev V.F. Porivnyal'ne doslidzhennya metodami spektrofluorimetrii ta protokovoi tsitometrii polyarizatsii plazmatichnoi i vnutrishn'oi mitohondrial'noi membran gladen'kom'yazovih klitin iz vikoristannyam potentsialchutlivogo zonda DiOS6(3) . Ukr. biohim. zhurn. 2011. 83, N 3. S. 99-105.
  2.  
  3. Kandaurova N.V., Chunihin O.Yu., Babich L.G., Shlikov S.G., Kosterin S.O. Modulyatori transmembrannogo al'tsiievogo obminu v mitohondriyah zminyuyut' ihnii gidrodinamichnii diametr . Tam samo. 2010. 82, N 6. S. 52-57.
  4.  
  5. Klyueva A.V., Levchuk Yu.N., Naboka Yu.N. Foton-korrelyatsionnaya spektroskopiya belkov . Tam samo. 2002. 74, N 5. S. 12-26.
  6.  
  7. Kostyuk P.G., Zima V.L. Miroshnichenko M.S., Shuba M.F. Biofizika. K.: Oberegi, 2001. 544 s.
  8.  
  9. Lebedev A.D., Levchuk Yu.N., Lomakin A.V., Noskin V.A. Lazernaya korrelyatsionnaya spektroskopiya v biologii. K.: Nauk. dumka, 1987. 256 s.
  10.  
  11. Tugai V.A. Regulyatornaya rol' protona v membrannih protsessah mishechnoi kletki. Tam samo, 1993. 183 s.
  12.  
  13. Tsimbalyuk O.V., Onufriichuk O.V., Veklich T.O., Cherenok S.O., Kal'chenko V.I., Miroshnichenko M.S., Kosterin S.O. Porivnyal'ne doslidzhennya vplivu ouabainu i kaliksaren bis-gidroksimetilfosfonovoi kisloti na aktivnist' Na+, K+-ATFazi ta mehanokinetiku protsesu «skorochennya-rozslablennya» gladen'kogo m'yazu . Fizika zhivogo. 2006. 14, N 1. S. 53-72.
  14.  
  15. Bazan C., Torres Barda D., Blomgren P., Paolini P. Image processing techniques for assessing contractility in isolated neonatal cardiac myocytes . Int. J. Biomed. Imaging. 2011. 2011, Article ID 729732. 9 p. 
  16.  
  17. Brainard A.M., Korovkina V.P., England S.K. Potassium channels and uterine function . Semin. Cell Dev. Biol. 2007. 18, N 3. P. 332-339.  CrossRef PubMed PubMedCentral
  18.  
  19. Delbridge L.M., Roos K.P. Optical methods to evalute the contractile function of unloaded isolated cardiac myocytes . J. Moll. Cell Cardiol. 1997. 29, N 1. P. 11-25. CrossRef PubMed
  20.  
  21. Dessouky D.A. Ultrastructural observations of the human uterine smooth muscle cells during gestation . Amer. J. Obstet. Gynecol. 1976. 125, N 8. P. 1099-1107. CrossRef  
  22. Gunst S.J., Tang D.D. The contractile apparatus and mechanical properties of airway smooth muscle . Eur. Respir. J. 2000. 15, N 3. P. 600-616. CrossRef PubMed
  23.  
  24. Haussinger D., Reinehr R., Schliess F. The hepatocyte integrin system and cell volume sensing . Acta Physiol. 2006. 187, Is. 1-2. P. 249-255.
  25.  
  26. Hoffman E.K., Dunham P.B. Membrane mechanisms and intracellular signalling in cell volume regulation . Int. Rev. Cytol. 1995. 161. P. 162-173. CrossRef  
  27. Jayakumar A.R., Lin M., Moriyama M. Na-K-Cl cotransporter-1 in the mechanism of ammonia-induced astrocyte swelling . J. Biol. Chem. 2008. 283, N 49. P. 33874-33882. CrossRef PubMed PubMedCentral
  28.  
  29. Kosterin S.A., Burdyga Th.V., Fomin V.P., Grover A.K. Mechanism of Ca2+-transport in myometrium. In: Control of Uterine Contractility / Eds. R.E. GarCeld, T.N.
  30.  
  31. ISSN 0201-8489 Fiziol. zhurn., 2013, T. 59, N 1 39 Yu.V. Danilovich, O.Yu. Chunihin, G.V.Danilovich Tabb . London, Tokyo: CRC Press, 1994. P. 129-153. 
  32.  
  33. Lewis R., Asplin K.E., Bruce G. The role of the membrane potential in chondrocyte volume regulation . J. Cell Physiol. 2011. 226, N 11. P. 2979-2986. CrossRef PubMed PubMedCentral
  34.  
  35. Maruyama I., Kobayashi M., Yoshida C., Momose K. Ultrastructure of single smooth muscle cells contracted by carbachol and calcium ion . J. Pharmacobiodyn. 1987. 10, N 8. P. 396-403.  CrossRef PubMed
  36.  
  37. Mollard P., Mironneau J., Amedee T. Electrophysiological characterization of single pregnant rat myometrial cells in short-term primary culture . Amer. J. Physiol. 1986. 19, N 1. P.C. 47-54. 
  38.  
  39. Momose K. Isolation, conCguration and contractile responses of single smooth muscle . Nihon Yakurigaku Zasshi. 1993. 101, N 3. P. 101-110. CrossRef PubMed
  40.  
  41. Remillard C.V., Yuan J.X. CLC-3: more than just a volumesensitive Clchannel . Br. J. Pharmacol. 2005. 145, N 1. P. 1-2. CrossRef PubMed PubMedCentral
  42.  
  43. Schliess F., Sinning R., Fisher R. Calcium-dependent activation of Erk-1 and Erk-2 after hypo-osmotic astrocyte swelling . Biochem. J. 1996. 320, Pt. 1. P. 167-171. CrossRef PubMed PubMedCentral
  44.  
  45. Shynlova O., Tsui P., Jaffer S., Lye S.J. Integration of endocrine and mechanical signals in the regulation of myometrial functions during pregnancy and labour . Eur. J. Obstet. Gynecol. Reprod. Biol. 2009. 144, N 1. P. 2-10. CrossRef PubMed
  46.  
  47. Sinning R., Schliess F., Kubitz R., Haussinger D. Osmosignalling in C6 glioma cells . FEBS Lett. 1997. 400, N 2. P. 163-167. CrossRef  
  48. Stephan vom Dahl, Kircheis G., Haussinger D. Hepatic encephalopathy as a complication of liver disease . World J. Gastrointerol. 2001. 7, N 2. P. 152-156. CrossRef PubMed PubMedCentral

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.