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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2013; 59(1): 32-39

Investigation of the changes in uterine myocytes size depending on contractile activity modulators by photon correlation spectroscopy

Danylovych IuV, Chunikhin OIu, Danylovych HV

    Kyiv Taras Shevchenko National University, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz59.01.032


Abstract

By using photon correlation spectroscopy the effect of contractile activity modulators of smooth muscle to the size (diameter) of rats uterine muscle cells was investigated. Cells were studied by using laser confocal microscopy as objects mostly oval or nearly oval form. The diameter of myocytes, assessed by photon correlation spectroscopy, was amounted 7-10 microm which is Consistent with the results obtained by using laser confocal microscopy. It is shown that the myoconstricted agents as Ca2+ ionophore A-23187 (10 microM) and K(+)-channels inhibitors (tetraethylammonium and 4-aminopyridine in a concentration of 1 mM) leading to decrease the diameter of the cells by 22%, 12% and 24% in average respectively. The ouabain, that reduces contractile activity of smooth muscle, leads to increase this parameter up 23% from control. Thus, decreasing/increasing the diameter of uterine myocytes correlates with their well known influence on myometrium constriction/relaxation.

Keywords: photon correlation spectroscopy, hydrodynamicdiameter, myocytes, myometrium, myoconctrictors, ouabain.

Full text: (PDF, in Ukrainian)

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