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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2012; 58(6): 57-66


Effect of hydrogen sulfideon isolated rat heart reactionunder volume loadand ischemia-reperfusion

T.V.Shimanskaya, Y.V.Goshovska,O.M.Semenikhina, V. F. Sagach

    O.O. Bogomolets Institute of Physiology, National Academyof Sciences of Ukraine, Kyiv.


Abstract

The present study was aimed to investigate the effect of H2S donor (NaHS) on heart function in conditions of functional loads and ischemia-reperfusion (I/R) injury by using Lan-gendorf isolated heart perfusion. NaHS (“Sigma”, 7,4 mg per kg) was dissolved in physiological solution and injected intraperitoneally 30 min before experiment. Rat isolated hearts were Langendorf-perfused and subjected to 20-minute non-fow ischemia followed by 40-minute reperfusion. The heart function was assessed by measuring the LVDP, dP/dt, coronary fow, heart rate. The opening of mitochondria permeability transition (MPT) pore was estimated by releasing of a stable factor with UV absorbance (lambda-max 250 nm) into the coronary out-fow probes during the initial phase of reperfusion. The results showed that NaHS pretreated hearts developed greater LVDP without decreasing of dP/dt min in response to an increase of left ventricle volume indicating greater functional reserves and effectiveness of Frank-Starling low realization. NaHS increased cardiac mitochondrial membrane potential but did not changed UCP3 gene expression. Signifcant post-ischemic recover of heart function in NaHS group was accompanied with tiny quantity of mitochondrial factor releasing comparing to I/R group (p<0.001). Thus, NaHS do provides cardioprotec-tive effect by inhibition of MPT pore opening.

Keywords: hydrogen sulfide, isolated heart, Frank-Starlinglow, ischemia-reperfusion, mitochondrial permeability transitionpore, uncoupling proteins, mitochondrial membranepotential.

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© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.