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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2012; 58(6): 57-66

Effect of hydrogen sulfide on isolated rat heart reaction under volume load and ischemia-reperfusion

Shymans'ka TV, Hoshovs'ka IuV, Semenikhina OM, Sahach VF.

    O.O. Bogomolets Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz58.06.057


Abstract

The present study was aimed to investigate the effect of H2S donor (NaHS) on heart function in conditions of functional loads and ischemia-reperfusion (I/R) injury by using Lan-gendorf isolated heart perfusion. NaHS (“Sigma”, 7,4 mg per kg) was dissolved in physiological solution and injected intraperitoneally 30 min before experiment. Rat isolated hearts were Langendorf-perfused and subjected to 20-minute non-fow ischemia followed by 40-minute reperfusion. The heart function was assessed by measuring the LVDP, dP/dt, coronary fow, heart rate. The opening of mitochondria permeability transition (MPT) pore was estimated by releasing of a stable factor with UV absorbance (lambda-max 250 nm) into the coronary out-fow probes during the initial phase of reperfusion. The results showed that NaHS pretreated hearts developed greater LVDP without decreasing of dP/dt min in response to an increase of left ventricle volume indicating greater functional reserves and effectiveness of Frank-Starling low realization. NaHS increased cardiac mitochondrial membrane potential but did not changed UCP3 gene expression. Signifcant post-ischemic recover of heart function in NaHS group was accompanied with tiny quantity of mitochondrial factor releasing comparing to I/R group (p<0.001). Thus, NaHS do provides cardioprotec-tive effect by inhibition of MPT pore opening.

Keywords: hydrogen sulfide, isolated heart, Frank-Starlinglow, ischemia-reperfusion, mitochondrial permeability transitionpore, uncoupling proteins, mitochondrial membranepotential.

Full text: (PDF, in Ukrainian)

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