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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2012; 58(6): 48-56


The role of nitric oxide and taurine in regulation of dogs gastric secretory function

Hrinchenko OA, Ianchuk PI.

    Peter Bogach Institute of Physiology ESC «Institute ofBiology» National Taras Shevchenko University of Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz58.06.048

Abstract

The infuence of taurine and endogenous nitric oxide on the gastric secretion and secretory process alterations by taurine at the nitric oxide synthesis blockade was investigated in chronic experiments on dogs with gastric fstulas. We determined the intensity of gastric secretion during 1,5 hours and quantita­tive content of hydrochloric acid, pepsine, total proteins and adenile system components. The NO-synthase inhibition by L-NAME evoked an increase of secreted gastric juice volume stimulated by histamine by 160% (Р<0,001), the gastric acid content – by 156,4% (Р<0,001), pepsine content – by 184,1% (Р<0,001) as compared with control. Taurine increases the volume of gastric juice stimulated by histamine on 129,3% (Р<0,001), hydrochloric acid – by 151,4% (Р<0,001), pepsine – by 172,2% (Р<0,001), total proteins – by 60,5% (Р<0,001) as compared with control. Blockade of nitric oxide synthesis did not alter the effect of taurine on histamine gastric secretion. The present results suggest that the endogenous nitric oxide provides an inhibitory infuence on gastric secretion and did not participate in realization of regulatory effects of taurine on gastric secretory function.

Keywords: nitric oxide, taurine, gastric secretion, hydrochloricacid, pepsine.

References

  1. Valenkevich VN Methodical developments on functional methods of research of a stomach and a gut. L., 1978. 31 p.
  2.  
  3. Grinchenko OA, Baranovsky VA, Veselsky SP, Yanchuk PI The effect of taurine on the content of components of the adenyl-catching system in gastric juice and blood plasma in dogs . Visn. Cherkasy. un-tu. The Biol series. science. 2011. 204. P. 31-40.
  4.  
  5. Grinchenko OA, Yanchuk PI Comparative analysis of the effect of different doses of taurine on histamine gastric secretion . Visn. Lviv. un-tu. The series is biological. 2011. 57. P. 222-235.
  6.  
  7. Kochetov GA Practical Guide to Enzymology . Ed. S.E. Severina. M.: Higher. school, 1980. 271 p.
  8.  
  9. Alada A.R., Salahdeen H.M., Akande O.O., Idolor G.O. In-fuence of nitric oxide on histamine and carbachol-induced gastric acid secretion in the common African toad bufo regularis . Niger J. Physiol. Scien. 2005. 20, N 1-2. P. 74-78.
  10.  
  11. Bentley J.K., Beavo J.A. Regulation and function of cyclic nucleotides . Curr. Opin. Cell Biol. 1992. 4. P. 233-240. CrossRef  
  12. Berg A., Kechagias S., Sjostrand S.E., Ericson A.C. Morphological support for paracrine inhibition of gastric acid secretion by nitric oxide in humans . Scand. J. Gastroen-terol. 2001. 36, N 10. P. 1016-1021. CrossRef PubMed
  13.  
  14. Berg A., Redeen S., Ericson A. C., Sj?strand S.E. Nitric oxide-an endogenous inhibitor of gastric acid secretion in isolated human gastric glands . BMC Gastroenterol. 2004. 4. P. 16. CrossRef PubMed PubMedCentral
  15.  
  16. Berg A., Redeen S., Grenegard M., Ericson A.-C., Sj?strand S.E. Nitric oxide inhibits gastric acid secretion by increasing intraparietal cell levels of cGMP in isolated human gastric glands . AJP: Gastrointest. Liver Physiol. 2005. 289. P. G1061-G1066. CrossRef PubMed
  17.  
  18. El-Abhar H.S. Coenzyme Q10: a novel gastroprotective ef­fect via modulation of vascular permeability, prostaglandin E2, nitric oxide and redox status in indomethacin-induced gastric ulcer model . Eur. J. Pharmacol. 2010. 649, N 1-3. P. 314-319. CrossRef PubMed
  19.  
  20. Garcia-Vitoria M., Garcia-Corchon C., Rodriguez J.A., Garcia-Amigot F., Burrell M.A. Expression of neuronal nitric oxide synthase in several cell types of the rat gastric epithelium . J. Histochem. and Cytochem. 2000. 48. P. 1111-1119. CrossRef PubMed
  21.  
  22. Grinchenko O.A., Yanchuk P.I. Pathways of taurine in-fuences on the gastric secretion . Intern. J. Physiol. and Pathophysiol. 2011. 2, N 3. P. 199-211. CrossRef  
  23. Hasebe K., Horie S., Komasaka M., Yano S., Watanabe K. Stimulatory effects of nitric oxide donors on gastric acid secretion in isolated mouse stomach . Eur. J. Pharmacol. 2001. 420, N 2-3. P. 159-164. CrossRef  
  24. Hasebe K., Horie S., Noji T., Watanabe K., Yano S. Stimulatory effects of endogenous and exogenous nitric oxide on gastric acid secretion in anesthetized rats . Nitric Oxide. 2005. 13, N 4. P. 264-271. CrossRef PubMed
  25.  
  26. Hasebe K., Horie S., Yano S., Watanabe K. Inhibitory effect of N(omega)-nitro-L-arginine on gastric secretion induced by secretagogues and vagal stimulation in the isolated stomach . Eur. J. Pharmacol. 1998. 350, N 2-3. P. 229-236. CrossRef  
  27. Hasebe K., Horie S., Yano S., Watanabe K. Stimulatory ef­fects of nitric oxide donors on histamine release in isolated rat gastric mucosal cells . Biol. Pharm. Bull. 2003. 26, N 7. P. 950-953. CrossRef PubMed
  28.  
  29. Horie S., Hasebe K., Koshikawa H. Involvement of nitric oxide, cyclic AMP and cyclic GMP in the peripheral control of gastric acid secretion via histamine-containing cells in mouse isolated stomach . Dig. Dis. Sci. 1998. 43. P. 23-43.
  30.  
  31. Huang K.H., Chang C.-C., Ho J.-D., Lu R.-H., Tsai L.H. Role of taurine on acid secretion in the rat stomach . J.Biomed. Scien. 2011. 18. P. 11. CrossRef PubMed PubMedCentral
  32.  
  33. Hunt G.M. Method for estimating peptic activity in gastric contents . Biochem. J. 1948. 42. P. 104-109. CrossRef PubMed PubMedCentral
  34.  
  35. Ito Y. , Okuda S., Ohkawa F., Kato S., Mitsufuji S., Yo-shikawa T., Takeuchi K. Dual role of nitric oxide in gastric hypersecretion in the distended stomach: inhibition of acid secretion and stimulation of pepsinogen secretion . Life Scien. 2008. 83, N 25-26. P. 886-892. CrossRef PubMed
  36.  
  37. Kato S., Kitamura M., Korolkiewicz R.P., Takeuchi K. Role of nitric oxide in regulation of gastric acid secretion in rats: effects of NO donors and NO synthase inhibitor . Brit. J. Pharmacol. 1998. 123. P. 839-846. CrossRef PubMed PubMedCentral
  38.  
  39. Kawauchi S., Sugamoto S., Furukawa O. Stimulation by nitric oxide of gastric acid secretion in bullfrog fundic mucosa in vitro . J. Physiol. Pharmacol. 2001. 52, N 1. P. 93-105.
  40.  
  41. Konturek J.W., Fischer H., Gromotka P.M. Endogenous nitric oxide in the regulation of gastric secretory and motor activity in humans . Aliment. Pharmacol. Ther. 1999. 13. P. 1683-1691. CrossRef PubMed
  42.  
  43. Motawi T.K., Abd Elgawad H.M., Shahin N.N. Modulation of indomethacin-induced gastric injury by spermine and taurine in rats . J. Biochem. Molec. Toxicol. 2007. 21, N 5. R. 280-288. CrossRef PubMed
  44.  
  45. Premaratne S. Xue C., McCarty J., Zaki M., McCuen R.W., Johns R.A., Schepp W., Neu B., Lippman R., Melone P.D., Schubert M.L. Neuronal nitric oxide synthase: expression in rat parietal cells . Amer. J. Physiol. Gastrointest. Liver Physiol. 2001. 280. P. G308-313. CrossRef PubMed
  46.  
  47. Takeuchi K., Araki H., Kawauchi S. Regulatory mecha­nism of acid secretion in the damaged stomach: role of endogenous nitric oxide . J. Gastroenterol. Hepatol. 2000. 15. P. D37-D45. CrossRef PubMed
  48.  
  49. Talarek S., Listos J., Fidecka S. Effect of nitric oxide synthase inhibitors on benzodiazepine withdrawal in mice and rats . Pharmacol. Rep. 2011. 63. P. 680-689. CrossRef  
  50. Tanaka J., Yuda Y. , Inouye S. The role of nitric oxide in the gastric acid secretion induced by ischemia-reperfusion in the pylorus-ligated rat . Eur. J. Pharmacol. 2001. 424, N 1. P. 69-74. CrossRef  
  51. Tsuchiya S., Horie S., Watanabe K. Stimulatory effects of centrally injected nitric oxide donors on gastric acid secretion in anesthetized rats . Jpn. J. Pharmacol. 2002. 89. P. 126-132. CrossRef PubMed
  52.  
  53. Vahedian Z., Nabavizadeh F., Keshavarz M., Vahedian J., Mirershadi F. Lead exposure changes gastric acid secretion in rat: role of nitric oxide (NO) . Acta Med. Iran. 2011. 49, N 1. P. 3-8.
  54.  

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