Participation of phosphatidylinositol-3`-kinase on signal transduction through galactosyl-containing glycoprotein receptors of segmentonuclear leukocytes under type 1 diabetes mellitus
N.O. Sybirna, І.V. Brodyak, M.L. Bars’ka,О.I. Vovk
Ivan Franko National University of Lviv, Ukraine;Institute of Cell Biology NAS of Ukraine, Lviv, Ukraine
DOI: https://doi.org/10.15407/fz58.06.009
Abstract
It is shown that reduction of b,D-galactosyl-containing carbohydrate determinants of glycoconjugates on the plasmatic membrane of segmentonuclear neutrophills of peripheral blood under type 1 diabetes mellitus (DM) is correlated with changes in aggregation of these cells and may cause their functional disorder. Changes in the parameters of ricin-induced neutrophil activation after inhibition of the phosphatidylinositol-3'-kinase (РІ-3’-kinase) enzyme with wortmannin indicated that the functional state of polymorphonuclear leukocytes is mediated by signaling pathways in which РІ-3’-kinase is involved. Thus, РІ-3’-kinase-dependent signal networks are involved in the processes of signal transduction through galactosyl-containing glycoprotein receptors into neutrophilic leukocytes. Inertness of the intensity formation in time of neutrophil granulocyte cell response on RCA-induced translocation of the p85a regulatory subunit of PI-3'-kinase from the cytosolic to the membrane fraction under type 1 DM is a consequence of changes in the number or structure of plasmatic galactosyl-containing glycoprotein receptors. The revealed changes may be etiologic premise of diabetic complications and chronic diseases that impair the functional condition of patients with type 1 DM. Key words: neutrophil granulocytes, b,D-galactosyl-containing carbohydrate determinants of glycoconjugates, РІ-3’-kinase, wortmannin, type 1 diabetes mellitus.
Keywords:
neutrophil granulocytes, B,D-galactosyl-containingcarbohydrate determinants of glycoconjugates, РІ-3’-kinase,wortmannin, type 1 diabetes mellitus.
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