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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2012; 58(6): 9-22

Participation of phosphatidylinositol-3`-kinase on signal transduction through galactosyl-containing glycoprotein receptors of segmentonuclear leukocytes under type 1 diabetes mellitus

N.O. Sybirna, І.V. Brodyak, M.L. Bars’ka,О.I. Vovk

    Ivan Franko National University of Lviv, Ukraine;Institute of Cell Biology NAS of Ukraine, Lviv, Ukraine


It is shown that reduction of b,D-galactosyl-containing carbohydrate determinants of glycoconjugates on the plasmatic membrane of segmentonuclear neutrophills of peripheral blood under type 1 diabetes mellitus (DM) is correlated with changes in aggregation of these cells and may cause their functional disorder. Changes in the parameters of ricin-induced neutrophil activation after inhibition of the phosphatidylinositol-3'-kinase (РІ-3’-kinase) enzyme with wortmannin indicated that the functional state of polymorphonuclear leukocytes is mediated by signaling pathways in which РІ-3’-kinase is involved. Thus, РІ-3’-kinase-dependent signal networks are involved in the processes of signal transduction through galactosyl-containing glycoprotein receptors into neutrophilic leukocytes. Inertness of the intensity formation in time of neutrophil granulocyte cell response on RCA-induced translocation of the p85a regulatory subunit of PI-3'-kinase from the cytosolic to the membrane fraction under type 1 DM is a consequence of changes in the number or structure of plasmatic galactosyl-containing glycoprotein receptors. The revealed changes may be etiologic premise of diabetic complications and chronic diseases that impair the functional condition of patients with type 1 DM. Key words: neutrophil granulocytes, b,D-galactosyl-containing carbohydrate determinants of glycoconjugates, РІ-3’-kinase, wortmannin, type 1 diabetes mellitus.

Keywords: neutrophil granulocytes, B,D-galactosyl-containingcarbohydrate determinants of glycoconjugates, РІ-3’-kinase,wortmannin, type 1 diabetes mellitus.


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