Українська Русский English

ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2012; 58(5): 22-27

The changes of the activity of NO-synthases and oxidative processes under conditions of 5-HT receptors activation in the stomach and large intestine in streptozocin-induced diabetes mellitus

Skliarov OIa, Detsyk OI.

    Danylo Halytsky Lviv National Medical University, Ukraine


We investigated the activity of NO-synthases, lipoperoxida-tion processes, antioxidant defense enzymes in the muscular layers of stomach and large intestine. The L-arginine concen­tration in blood plasma was also monitored under conditions of 2-weeks activation of 5-HT4 receptors by mosaprid in streptozocin-induced diabetes mellitus. We showed that the onset of diabetes mellitus is accompanied by a 2.4-2.8-fold increase in the activity of inducible NO-synthase and a 23-40% increase in the SOD activity. The nitric oxide content and TBA products in the muscular layers of stomach and large intestine were increased, whereas the motor-evacuational func­tion of the stomach and large intestine decreased. Activation of 5-HT4 receptors by mosaprid under conditions of diabetes mellitus decreased the activity of inducible NO-synthase, the lipoperoxidation processes, nitrite anion content and TBA products in muscular layers of stomach and large intestine by 23%. At the same time, we observed an increase in the motor-evacuational function of stomach and large intestine without affecting the blood sugar level.

Keywords: NO-synthase activity, lipoperoxidation processes,nitrite anion, muscular layers of the stomach and large intestine,streptozocin-induced diabetes mellitus, 5-HT4 receptors, mosaprid.


  1. Aleynikova TL, Rubtseva GV, Pavlova NA A guide to practical training in biochemistry. M .: Medicine, 2000. 128 p.
  2. Decik O., Fomenko I., Sklyarov O. Activity of NO synthases, nitrogen oxide content and lipo-peroxidation processes in stomach and colon under conditions of streptozotocin-induced hyperglycemia . Experim. physiology and biochemistry. 2011. 3 (55). pp. 7-12.
  3. Drill VR Basic mechanisms of occurrence and development of diabetic complications: the role of nitric stress . Biol. studio. 2010. 4. N 2. P.141-158.
  4. Koroluk MA, Ivanova LI, Mayorova IG, Tokarev VE A method for determining catalase activity . Lab. business. 1988. N 1. P.16-19.
  5. Sumbaev VV Yasinskaya IM The effect of DDT on the activity of nitric oxide synthase in the liver, lungs and brain of rats . Sovrem. prob. toxicology. 2000. N 3. P.3-7.
  6. Timurbulatov MA, Seleznev EI The method of increasing free radical oxidation of lipid-containing blood components and its diagnostic value . Lab. business. 1981.- N 4. P.209-211.
  7. Chevari S., Andyal T., Stranger J. Determination of antioxidant parameters of blood and their diagnostic value in the elderly . Ibid. 1991. N 10. P.9-13.
  8. Shadrin O.G., Gotsulyak S.M., Tsipkun A.G. Induced contractile activity of smooth muscle of rat colon under NO-synthase blockade . Physiol. . 2004. 50, N 4. P.123-127.
  9. Cellek S. Point of return for nitrergic nerves in diabetes: a new insight into diabetic complications . Curr.Farm. Des. 2004. 10, N 29. S.3683-3685.
  10. De Maeyer J.H., Lefebvre R.A., Schuurkes J.A. 5-HT4 receptor agonists: similar but not the same . Neurogas-troenterol. Motil. 2008. 20, N 2. P.99-112.
  11. Green L.C., A.W. David. Analysis of nitrate, nitrite and (1515) nitrate in biological fuids . Anal. Biochem. 1982. 126. R.131-138.
  12. Inui A., Yoshikawa T., Nagai R., Yoshida N., Ito T. Effects of mosapride citrate, a 5-HT4 receptor agonist, on colonic motility in conscious guinea pigs . Jpn. J. Pharmacol. 2002. 90. P. 313 320.
  13. Kim H.S., Choi E.J., Park H. The effect of mosapride citrate on proximal and distal colonic motor function in the guinea-pig in vitro . Neurogastroenterol. Motil. 2008. 20, N 2. P.169-176.
  14. Lanas A. Role of nitric oxide in the gastrointestinal tract . Arthritis Res. & Therap. 2008. 10 (Suppl 2). S4.
  15. Meile T., Glatzle J., Habermann F.M., Kreis M.E., Zittel T.T. Nitric oxide synthase inhibition results in immediate postoperative recovery of gastric, small intestinal and colonic motility in awake rats . Int. J. Colorectal. Dis. 2006. 2. P. 121-129.
  16. Mine Y., Yoshikawa T., Oku S., Nagai R, Yoshida N., Hosoki K. Comparison of effect of mosapride citrate and existing 5-HT4 receptor agonists on gastrointestinal motility in vivo and in vitro. J. Pharmacol. Exp. Therap. 1997. 283, N 3. P. 1000-1008.
  17. Nishio H., Hayashi Y., Terashima S., Takeuchi K. Role of endogenous nitric oxide in mucosal defence of infamed rat stomach following iodoacetamide treatment . Life Sci. 2006. 79, N 16. P. 1523-1530.
  18. Onori L., Aggio A., D’Alo S., Muzi P., Cifone M.G., Mellillo G., Ciccocioppo R., Taddei G., Frieri G., Latella G. Role nitric oxide in the impairment of circular muscle contractility of distended, uninfamed mid-colon in TNBS-induced acute distal colitis in rats . Word. J. Gastrointes­tinal. 2005. 11, N 36. S.5677-5684.
  19. Qu Z.D., Thacker M., Baqyanszki P. , Bagyanszki M., Epstein M.L., Furness J.B. Immunohistochemical analisis of neuron types in the mouse small intestine . Cell Tissue Res. 2008. 334, N 2. S.147-161.
  20. Rosztoczy A., Roka R., Varkonyi T. T., Lengyel C., Izbeki F., Lonovics J., Wittmann T. Regional differences in the manifestation of gastrointestinal motor disorders in type 1 diabetic patients with autonomic neuropathy . Z. Gas-troeterol. 2004. 42, N 11. S.1295-1300.
  21. Seven A., Guzel S., Seymen O., Civelek S., Bolayirli M., Yigit G., Burcak G. Nitric oxide synthase inhibition by L-NAME in streptozotocin induced diabetic rats: impacts on oxidative stress . Tohoku J. Exp. Med. 2003. 199, N 4. P.205-210.
  22. Toda N., Herman A.G. Gastrointestinal function regulation by nitrergic efferent nerves . Pharmacol. Rev. 2005. 57, N 3. S.315-338.
  23. Van den Oever I., Raterman H.G., Nurmohamed M.T., Simsek S. Endothelial dysfunction, infammation, and apoptosis in diabetes mellitus . Mediators of Infamma-tion. 2010. Published online, 10.1155. 2010. 792393.
  24. Wallace J.L., Ma L. Infammatory mediators in gastrointes­tinal defense and injury . Exp. Biol. Med. 2001. 226, N 11. P. 1003-1015.
  25. Zandecki M., Vanden Berghe P., Depoortere I., Geboes K., Peeters T., Janssens J., Tack J. Characterization of myenteric neuropathy in the jejunum of spontaneously diabetic BB-rats . Neurogastroenterol. Motil. 2008. 20, N 7. S.818-828.
  26. Zhao J., Yang J., Gregersen H. Biomechanical and morpho-metric intestinal remodeling during experimental diabetes in rats . Diabetologia. 2003. 46, N 12. P. 1688-1697.

© National Academy of Sciences of Ukraine, Bogomoletz Institute of Physiology, 2014-2019.