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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2012; 58(5): 14-21

The polymorphism of matrix Gla-protein gene in ischemic atherothrombotic stroke patients

Harbuzova VIu, Matlaĭ OI, Ataman IuO, Dubovyk IeI, Borodenko AO, Obukhova OA, Ataman OV

    Sumy State University, Ukraine


Matrix Gla protein (MGP) gene allelic polymorphism in 170 patients with ischemic atherothrombotic stroke (IATS) and in 124 healthy people was determined. It was shown that in patients with IATS interrelation of main homozygotes, heterozygotes and minor homozygotes is 61,2, 31,2, 7,6% for T-138 -> C (rs1800802) promoter polymorphism (in control – 59,7, 35,6, 4,8%, P=0,521 by x2-test); 35,9, 48,8, 15,3% for G-7 -> A (rs1800801) polymorphism of promotor (in control – 43,5, 50,0, 6,5%, P=0,051); 39,4, 48,8, 11,8% for Thr83 -> Ala (rs4236) polymorphism of 4 exon (in control – 34,7, 53,2, 12,1%, P=0,701). Signifcant differences in the frequency of G-7 -> A polymorphism between patients with IATS and control group were revealed only for women (P=0.022). The results obtained suggest that the A/A-variant of MGP gene promotor (G-7 -> A polymorphism) is associated with an increased risk of IATS in female persons in the Ukrainian population.

Keywords: matrix Gla protein, gene polymorphism, ischemicstroke.


  1. Garbuzova V.Yu., Guryanova V.L., Parkhomenko A.N., Dosenko V.E., Ataman O.V. The frequency of allelic variants of the matrix Gla-protein (MGP) gene in patients with acute coronary syndrome . Fiziol. Zh. 2011. 57, N 3. P. 16-24.
  3. Torshin I.Yu., Gromova OA, Nikonov AA Genes and cerebrovascular pathology (genes and nucleotide polymorphisms in certain types of physiological shifts and pathological processes) . Journal. neurol. psychiatry. 2009. 109, N 5. pp. 77-85.
  5.  Abedin M., Tintut Y., Demer L.L. Vascular calci­fication. Mechanisms and clinical ramifications . Arterioscler. Thromb. Vasc. Biol. 2004. 24. P. 1161-1170. CrossRef PubMed
  7. Acar A., Cevik M.U., Arikanoglu A., Evliyaoglu O., Basarili M.K., Uzar E., Ekici F., Yucel Y., Tasdemir N. Serum levels of calcifcation inhibitors in patients with intracerebral hemorrhage . Int. J. Neurosci. 2011. Nov 24 [Epub ahead of print]. CrossRef PubMed
  9. Adams H.P., Bendixen B.H., Kappelle L.J., Biller J., Love B.B., Gordon D.L., Marsh E.E. Classifcation of subtype of acute ischemic stroke. Defnitions for use in a multicenter clinical trial. TOAST. Trial of Org 10172 in Acute Stroke Treatment . Stroke. 1993. 24. P. 35-41. CrossRef PubMed
  11. Allison M.A., Wright C.M. Age and gender are the strongest clinical correlates of prevalent coronary calcifcation (R1) . . Int. J. Card. 2005. 98. P . 325-330. CrossRef PubMed
  13. Bos D., Ikram M.A., Elias-Smale S.E., Krestin G.P., Hofman A., Witteman J.C., van der Lugt A., Vernooij M.W . Calcifcation in major vessel beds relates to vascular brain disease . Arterioscler. Thromb. Vasc. Biol. 2011. 31. P . 2331-2337. CrossRef PubMed
  15. Braam L.A., Dissel P. , Gijsbers B.L., Spronk H.M.H., Hamulyak K., Soute B.A.M., Debie W., Vermeer C. Assay for human matrix Gla protein in serum. Potential applica­tions in the cardiovascular feld . Ibid. 2000. 20. P. 1257-1261. CrossRef PubMed
  17. Brancaccio D., Biondi M.L., Gallieni M., Turri O., Galassi A., Cecchini F., Russo D., Andreucci V. , Cozzolino M. Ma­trix GLA protein gene polymorphisms: clinical correlates and cardiovascular mortality in chronic kidney disease patients . Amer. J. Nephrol. 2005. 25. P. 548-552. CrossRef PubMed
  19. Crosier M.D., Booth S.L., Peter I., Dawson-Hughes B., Price P.A., O'Donnell C.J., Hoffmann U., Wiilliamson M.K., Ordovas J.M. Matrix Gla protein polymorphisms are associated with coronary artery calcifcation . J. Nutr. Sci. Vitaminol. 2009. 55. P. 59-64. CrossRef PubMed PubMedCentral
  21. Detrano R.C., Wong N.D., Doherty T.M., Shavelle R. Prognostic signifcance of coronary calcifc deposits in a symptomatic high-risk subjects . Ame r. J. Med. 1997. 102. P. 344-349. CrossRef  
  22. Doherty T.M., Fitzpatrick L.A., Inoue D., Qiao J.H., Fishbein M.C., Detrano R.C., Shah P.K., Rajavashisth T.B. Molecular, endocrine, and genetic mechanisms of arterial calcifcation . Endocrine Rev. 2004. 25. P. 629-672. CrossRef PubMed
  24. Farzaneh-Far A., Davies J.D., Braam L.A., Spronk H.M., Proudfoot D., Chan S.W., O'Shaughnessy K.M., Weissberg P.L., Vermeer C., Shanaham C.M. A Polymorphism of the human matrix g-carboxyglutamic acid protein promoter alters binding of an activating protein-1 complex and is associated with altered transcription and serum levels . J. Biol. Chem. 2001. 276. P. 32466-32473. CrossRef PubMed
  26. Fraser J.D., Price P.A. Lung, heart, and kidney express high levels of mRNA for the vitamin K-dependent matrix Gla protein . Ibid. 1988. 263. P. 11033-11036.
  28. Fuessl H.S., Schalzky H., Schewe S., Frey K.W., Gobel F.-D. Zur Pathogenese und klinischen Bedeutung der Monckebergschen Mediaverkalkung . Klin. Wochenschr. 1985. 63. S. 211-216. CrossRef PubMed
  30. Guzman R.J. Clinical, cellular, and molecular aspects of arterial calcifcation . J. Vasc. Surg. 2007. 45 (Suppl A). P. A57-A63. CrossRef PubMed PubMedCentral
  32. Hernandez-Pacheco G., Murguia L.E., Rodriguez-Perez J.M., Fragoso J.M., Perez-Vielma N., Martinez-Rodriguez N., Granados J., Vargas-Alarcon G. Matrix gamma-car-boxyglutamic acid protein (MGP) G-7A and T-138C gene polymorphisms in Indian (Mayo and Teenek) and Mestizo populations from Mexico . Hum. Biol. 2005. 77. P. 385-391. CrossRef PubMed
  34. Herrmann S.M., Whatling C., Brand E., Nikaud V., Gari-epy J., Simon A., Evans A., Ruidavets L.B., Arveiler D., Luc G., Tiret L., Henney A., Cambien F. Polymorphisms of the human matrix Gla protein (MGP) gene, vascular calcifcation, and myocardial infarction . Arterioscler. Thromb. Vasc. Biol. 2000. 20. P. 2386-2393. CrossRef PubMed
  36. Iribarren C., Sidney S., Sternfeld B., Browner W.S. Cal-cifcation of the aortic arch: risk factors and association with coronary heart disease, stroke, and peripheral vascular disease . JAMA. 2000. 283. P. 2810-2815. CrossRef PubMed
  38. Jayalath R.W., Mangan S.H., Golledge J. Aortic cal­cification . Eur. J. Endovasc. Surg. 2005. 30. P. 476-488. CrossRef PubMed
  40. Johnson R.C., Leopold J.A., Loscalzo J. Vascular calcification. Pathobiological mechanisms and clini­cal implications . Circulat. Res. 2006. 99. P. 1044-1059. CrossRef PubMed
  42. Jono S., Vermeer C., Dissel P., Hasegava K., Shioi A., Taniwaki H., Kizu A., Nishizawa Y. , Saito S. Ma­trix Gla protein is associated with coronary artery calcification as assessed by electron-beam computed tomography . Thromb. Haemost. 2004. 91. P. 790-794. CrossRef PubMed
  44. Kobayashi N., Kitazawa R., Maeda S., Schurgers L.J., Kitazawa S. T-138C polymorphism of matrix Gla protein promoter alters its expression but is not directly associated with atherosclerotic vascular calcifcation . Kobe J. Med. Sci. 2004. 50. P. 69-81.
  46. Lehto S., Niskanen L., Suhonen M., Ronnemaa T., Laasko M. Medial artery calcifcation. A neglected harbinger of cardiovascular complications in non-insulin-dependent diabetes mellitus . Arterioscler. Thromb. Vasc. Biol. 1996. 16. P. 978-988. CrossRef PubMed
  48. O'Donnell C.J., Kyla Shea M., Price P.A., Gagnon D.R., Wilson P.W.F., Larson M.G., Kiel D.P., Hoffmann U., Ferencik M., Clouse M.E., Williamson M.K., Cupples L.A., Dawson-Hughes B., Booth S.L. Matrix Gla protein is associated with risk factors for atherosclerosis but not with coronary artery calcifcation) . Arterioscler. Thromb. Vasc. Biol. 2006. 26. P. 2769-2774. CrossRef PubMed PubMedCentral
  50. Price P.A., Faus S.A., Williamson M.K. Warfarin-iduced artery calcifcation is accelerated by growth and vitamin D . Ibid. 2000. 20. P. 317-327. CrossRef PubMed
  52. Price P.A., Urist M.R., Otawara Y. Matrix Gla protein, a new g-carboxyglutamic acid-containing protein which is associated with the organic matrix of bone . Biochem. and Biophys. Res. Commun. 1983. 117. P. 765-771. CrossRef  
  53. Price P.A., Williamson M.K. Primary structure of bovine matrix Gla protein, a new vitamin K-dependent bone protein . J. Biol. Chem. 1985. 260. P. 14971-14975.
  55. Proudfoot D., Shanahan C.M. Molecular mechanisms mediating vascular calcifcation: role of matrix Gla protein . Nephrology (Carlton). 2006. 11. P. 455-461. CrossRef PubMed
  57. Taylor B.C., Schreiner P.J., Doherty T.M., Fornage M., Carr J.J., Sidney S. Matrix Gla protein and osteopontin genetic associations with coronary artery calcifcation and bone density: the CARDIA study . Hum. Genet. 2005. 11 6. P. 525-528. CrossRef PubMed
  59. Wayhs R., Zelinger A., Raggi P. High coronary artery calcium scores pose an extremely elevated risk for hard events . J. Amer. Col. Card. 2002. 39. P. 225-230. CrossRef  

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