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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2012; 58(5): 3-13

Effect of the Na+, K(+)-ATPase modulation in neurons of the medulla oblongata on hemodynamic effects in spontaneously hypertensive rats

Shapoval LM, Dmytrenko OV, Vavilova HL, Pobigaĭlo LS, Stepanenko LH, Radchenko NV, Davydovs'ka TL, Sagach VF

  1. O.O.Bogomoletz Institute of Physiology, National Academy of Sciences of Ukraine, Kyiv, Ukraine
  2. National Taras Shevchenko University, Kyiv, Ukraine


The study was conducted in normotensive and spontaneously hypertensive rats anesthetized with urethane (1600 mg / kg of animal weight, intraperitoneally). It has been shown that in normotensive rats, injections of a specifc inhibitor of Na +, K+-ATPase ouabain (10-8–10-5 mol / l) in the populations of the neurons within nucleus of the solitary tract (NTS), paramedian reticular nucleus (PMn) and lateral reticular nucleus (LRN) were accompanied by the development of the hypertensive responses in a dose-dependent fashion. These data suggest that Na+, K+-ATPase of the neuron somatic membranes in the medullary cardiovascular nuclei is involved in neural control of the cardiovascular function, and its inhibition by microinjections of ouabain promotes the development of hypertension. In contrast to normotensive rats, ouabain injected in the medullary nuclei of spontaneously hypertensive animals induced either enhanced hypertensive or hypotensive responses. Biochemical analysis revealed that the activity of Na+, K+-ATPase in the microsomal fraction of the medulla oblongata of spontaneously hypertensive rats signifcantly exceeded its activity in the medulla oblongata of normotensive animals. Possible mechanisms of ouabain effects in spontaneously hypertensive rats have being discussed. Activation of Na+, K+-ATPase activity of the cardiovascular neurons with asparkam injections in the medullary nuclei resulted in hypotensive responses in both normotensive and spontaneously hypertensive rats.

Keywords: Na[sup]+[/sup], K[sup]+[/sup]-ATPase, ouabain, asparkam, medullary cardiovascular neurons, normotensive and spontaneously hypertensive rats.


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