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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2012; 58(4): 21-29


Hypoxic preconditioning preventsthe induction and activationof 5-lipoxygenase during ischemiaand reperfusion of rat heart

A.G. Portnychenko, M.I. Vasylenko,O.O.Moybenko

  1. O.O.Bogomoletz Institute of Physiology, NAS of Ukraine,
  2. International Centre for Astronomical and Medico-Ecological Research, NAS of Ukraine, Kyiv, Ukraine


Abstract

Male Wistar rats were subjected to hypoxic preconditioning (10% O2 in nitrogen for 3 h). In 24 h heart were isolated and subjected to 30 min ischemia and 40 min reperfusion. Changes in expression of 5-lipoxygenase (5-LO) protein in rat heart ven­tricles, and in myocardial subcellular fractions were evaluated by Western blotting. It was found that hypoxic precondition­ing attenuated reperfusion damage of cardiomyocytes with reducing the release of LDH by 27.6%. After ischemia and reperfusion, expression of 5-LO was 10.5-fold elevated in the left ventricle and 14.3-fold – in the right one. During ischemia and reperfusion occurred gradual translocation of 5-LO protein in nuclear subcellular compartment, more expressive in the left ventricle. Hypoxic preconditioning did not signifcant increase in 5-LO expression, but fully prevented its growth in the following ischemia-reperfusion, and partly reduced protein translocation at reperfusion in the left ventricle. Thus, hypoxic preconditioning limits proinfammatory effects of ischemia and reperfusion in myocardium, preventing the increase in expres­sion of 5-LO, and reducing the alteration of cardiomyocytes.

Keywords: myocardium, hypoxic preconditioning, ischemiaand reperfusion, 5-lipoxygenase.

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