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ISSN 2522-9028 (Print)
ISSN 2522-9036 (Online)
DOI: https://doi.org/10.15407/fz

Fiziologichnyi Zhurnal

is a scientific journal issued by the

Bogomoletz Institute of Physiology
National Academy of Sciences of Ukraine

Editor-in-chief: V.F. Sagach

The journal was founded in 1955 as
1955 – 1977 "Fiziolohichnyi zhurnal" (ISSN 0015 – 3311)
1978 – 1993 "Fiziologicheskii zhurnal" (ISSN 0201 – 8489)
1994 – 2016 "Fiziolohichnyi zhurnal" (ISSN 0201 – 8489)
2017 – "Fiziolohichnyi zhurnal" (ISSN 2522-9028)

Fiziol. Zh. 2012; 58(4): 21-29


Hypoxic preconditioning prevents the induction and activation of 5-lipoxygenase during ischemia and reperfusion of rat heart

Portnichenko AG, Vasilenko MI, Moĭbenko AA.

  1. O.O.Bogomoletz Institute of Physiology, NAS of Ukraine
  2. International Centre for Astronomical and Medico-Ecological Research, NAS of Ukraine, Kyiv, Ukraine
DOI: https://doi.org/10.15407/fz58.04.021

Abstract

Male Wistar rats were subjected to hypoxic preconditioning (10% O2 in nitrogen for 3 h). In 24 h heart were isolated and subjected to 30 min ischemia and 40 min reperfusion. Changes in expression of 5-lipoxygenase (5-LO) protein in rat heart ven­tricles, and in myocardial subcellular fractions were evaluated by Western blotting. It was found that hypoxic precondition­ing attenuated reperfusion damage of cardiomyocytes with reducing the release of LDH by 27.6%. After ischemia and reperfusion, expression of 5-LO was 10.5-fold elevated in the left ventricle and 14.3-fold – in the right one. During ischemia and reperfusion occurred gradual translocation of 5-LO protein in nuclear subcellular compartment, more expressive in the left ventricle. Hypoxic preconditioning did not signifcant increase in 5-LO expression, but fully prevented its growth in the following ischemia-reperfusion, and partly reduced protein translocation at reperfusion in the left ventricle. Thus, hypoxic preconditioning limits proinfammatory effects of ischemia and reperfusion in myocardium, preventing the increase in expres­sion of 5-LO, and reducing the alteration of cardiomyocytes.

Keywords: myocardium, hypoxic preconditioning, ischemiaand reperfusion, 5-lipoxygenase.

References

  1. Portnichenko AG, Vasilenko MI, Moibenko O.O. Influence of acute hypoxic hypoxia on the induction of nitric oxide synthase in rats . Fiziol. zh. 2003. 49, N 3. P.47-49.
  2.  
  3. Portchenko AG, Vasilenko MI, Portnichenko VI, Moibenko AA Acute hypoxic hypoxia as an inducer of delayed cardioprotection in rats . Hypoxia, an automated analysis of hypoxic states . Sat. works under the editorship. A.Z.Kolchinskaya. M. Nalchik, 2005. Vol. 1. P.185-190.
  4.  
  5. Endogenous mechanisms of cardioprotection as a basis of pathogenetic therapy of heart diseases . Ed. A.A. Moibenko, V.E. Dosenko, AN Parkhomenko. K .: Scientific-Production Company "Scientific Thought" Publishing House of NAS of Ukraine, 2008. 518 p.
  6.  
  7. Dincbas-Renqvist V. , Pepin G., Rakonjac M., Plante I., Ouellet D.L., Hermansson A., Goulet I., Doucet J., Samuelsson B., Radmark O., Provost P. Human dicer C-terminus functions as a 5-lipoxygenase binding domain . . Biochim. Biophys. Acta. 2009. 1789. P. 99-108. CrossRef PubMed PubMedCentral
  8.  
  9. Freiberg J.J., Tybjaerg-Hansen A., Sillesen H., Jensen G.B., Nordestgaard B.G. Promotor polymorphisms in leukot-riene C4 synthase and risk of ischemic cerebrovascular disease . Arterioscler. Thromb. Vasc. Biol. 2008. 28, N 5. P. 990-996. CrossRef PubMed
  10.  
  11. Fryer R.M., Patel H.H., Hsu A.K., Gross G.J. Stress-acti­vated protein kinase phosphorylation during cardioprotec-tion in the ischemic myocardium . Amer. J. Physiol. Heart Circ. Physiol. 2001. 281, 3. P. H1184-H1192. CrossRef PubMed
  12.  
  13. Gonsalves C.S., Kalra V.K. Hypoxia-mediated expression of 5-lipoxygenase-activating protein involves HIF-1alpha and NF-kappaB and microRNAs 135a and 199a-5p . J. Immunol. 2010. 184, N 7. P. 3878-3888. CrossRef PubMed
  14.  
  15. Hakonarson H. Role of FLAP and PDE4D in myocardial infarction and stroke: target discovery and future treatment options . Curr. Treat. Options Cardiovasc. Med. 2006. 8, N 3. P. 183-192. CrossRef PubMed
  16.  
  17. Hartiala J., Li D., Conti D.V., Vikman S., Patel Y. , Tang W.H., Brennan M.L., Newman J.W., Stephensen C.B., Armstrong P., Hazen S.L., Allayee H. Genetic contribution of the leukotriene pathway to coronary artery disease . Hum. Genet. 2011. 129, N 6. P.617-627. CrossRef PubMed PubMedCentral
  18.  
  19. Huang H., Zeng Z., Li J., Zhang L., Chen Y. Variants of arachidonate 5-lipoxygenase-activating protein (ALOX5AP) gene and risk of coronary heart disease: A meta-analysis . Arch. Med. Res. 2010. 41, N 8. P. 634-641. CrossRef PubMed
  20.  
  21. Kido M., Otani H., Kyoi S., Sumida T., Fujiwara H., Okada T., Imamura H. Ischemic preconditioning-mediated restoration of membrane dystrophin during reperfusion correlates with protection against contraction-induced myocardial injury . Amer. J. Physiol. Heart Circ. Physiol. 2004. 287, N 1. P. H81-H90. CrossRef PubMed
  22.  
  23. Lisovyy O.O., Dosenko V.E., Nagibin V.S., Tumanovska L.V., Korol M.O., Surova O.V., Moibenko O.O. Cardio-protective effect of 5-lipoxygenase gene (ALOX5) silencing in ischemia-reperfusion . Acta Biochim. Pol. 2009. 56, N 4. P.687-694. CrossRef PubMed
  24.  
  25. Poeckel D., Funk C.D. The 5-lipoxygenase. leukotriene pathway in preclinical models of cardiovascular disease . Cardiovasc. Res. 2010. 86, N 2. P. 243-253. CrossRef PubMed
  26.  
  27. Portnychenko A.G., Harmatina O.Yu., Kotsuruba A.V., Moybenko O.O. The role of nitric oxide in endotoxin-induced cardiodepression . Exp. Clin. Cardiol. 2005. 10, N 4. P. 223-228.
  28.  
  29. Prasad N.S., Raghavendra R., Lokesh B.R., Naidu K.A. Spice phenolics inhibit human PMNL 5-lipoxygenase . Prostaglandins Leukot. Essent. Fatty Acids. 2004. 70, N 6. P. 521-528. CrossRef PubMed
  30.  
  31. Radmark O., Samuelsson B. Regulation of the activity of 5-lipoxygenase, a key enzyme in leukotriene biosynthesis . Biochem. Biophys. Res. Commun. 2010. 396, N 1. P. 105-110. CrossRef PubMed
  32.  
  33. Recchiuti A., Krishnamoorthy S., Fredman G., Chiang N., Serhan C.N. MicroRNAs in resolution of acute infamma-tion: identifcation of novel resolvin D1-miRNA circuits . FASEB J. 2011. 25. P. 544-560. CrossRef PubMed PubMedCentral
  34.  
  35. Shinmura K., Tang X.L., Wang Y., Xuan Y.T., Liu S.Q., Takano H., Bhatnagar A., Bolli R.Cyclooxygenase-2 mediates the cardioprotective effects of the late phase of ischemic preconditioning in conscious rabbits . Proc. Natl. Acad. Sci. USA. 2000. 97, N 18. P.10197-10202. CrossRef PubMed PubMedCentral
  36.  
  37. Tsai A.K., Li N., Hanson N.Q., Tsai M.Y., Tang W. Associations of genetic polymorphisms of arachidonate 5-lipoxygenase-activating protein with risk of coronary artery disease in a European-American population . Atherosclerosis. 2009. 207, N 2. P. 487-491. CrossRef PubMed
  38.  
  39. Xi L., Tekin D., Gursoy E., Salloum F., Levasseur J.E., Kukreja R.C. Evidence that NOS2 acts as a trigger and mediator of late preconditioning induced by acute systemic hypoxia . Amer. J. Physiol. Heart Circ. Physiol. 2002. 283, N 1. P. H5-H12. CrossRef PubMed
  40.  
  41. Ye Y. , Lin Y., Perez-Polo J.R., Uretsky B.F., Ye Z., Tieu B.C., Birnbaum Y. Phosphorylation of 5-lipoxygenase at ser523 by protein kinase A determines whether piogli-tazone and atorvastatin induce proinfammatory leukotri-ene B4 or anti-infammatory 15-epi-lipoxin A4 production . J. Immunol. 2008. 181, N 5. P.3515-3523. CrossRef PubMed
  42.  
  43. Zhao L., Moos M.P., Grabner R., Pedrono F., Fan J., Kaiser B., John N., Schmidt S., Spanbroek R., Lotzer K., Huang L., Cui J., Rader D.J., Evans J.F., Habenicht A.J., Funk C.D. The 5-lipoxygenase pathway promotes pathogenesis of hyperlipidemia-dependent aortic aneurysm . Nat. Med. 2004. 10, N 9. P. 966-973. CrossRef PubMed
  44.  

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